Neurospora crassa mat A-2 and mat A-3 proteins weakly interact in the yeast two-hybrid system and affect yeast growth
Mating-type genes control the entry into the sexual cycle, mating identity and sexual development in fungi. The mat A-2 and mat A-3 genes, present in the mat A idiomorph of the filamentous fungus Neurospora crassa, are required for post-fertilization functions but are not essential for mating identi...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2009-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200023 |
| _version_ | 1819108523552800768 |
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| author | Carla C. da Silva Rosana C. Cruz Mônica Bucciarelli-Rodriguez Adlane Vilas-Boas |
| author_facet | Carla C. da Silva Rosana C. Cruz Mônica Bucciarelli-Rodriguez Adlane Vilas-Boas |
| author_sort | Carla C. da Silva |
| collection | DOAJ |
| description | Mating-type genes control the entry into the sexual cycle, mating identity and sexual development in fungi. The mat A-2 and mat A-3 genes, present in the mat A idiomorph of the filamentous fungus Neurospora crassa, are required for post-fertilization functions but are not essential for mating identity. Their putative roles as transcription factors are based on the similarity of mat A-2 with the Podospora anserina SMR1 gene and an HMG motif present in the mat A-3 gene. In this work the yeast two-hybrid system was used to identify transcriptional activity and protein-protein interaction of N. crassa mat A-2 and mat A-3 genes. We observed that the mat A-3 protein alone is capable of weakly activating transcription of yeast reporter genes; it also binds with low specificity to the GAL1 promoter sequence, possibly due to its HMG domain. Our results also indicate that mat A-3 is capable to form homodimers, and interact with mat A-2. Interference on yeast growth was observed on some transformants suggesting a toxic action of the mat A-2 protein. Our data on pattern of interactions of mat proteins contributes towards understanding the control of vegetative and sexual cycles in filamentous fungi. |
| first_indexed | 2024-12-22T03:11:17Z |
| format | Article |
| id | doaj.art-6f91c1d21ba2470db53d239a13e3be9d |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-22T03:11:17Z |
| publishDate | 2009-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-6f91c1d21ba2470db53d239a13e3be9d2022-12-21T18:40:56ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852009-01-0132235436110.1590/S1415-47572009000200023Neurospora crassa mat A-2 and mat A-3 proteins weakly interact in the yeast two-hybrid system and affect yeast growthCarla C. da SilvaRosana C. CruzMônica Bucciarelli-RodriguezAdlane Vilas-BoasMating-type genes control the entry into the sexual cycle, mating identity and sexual development in fungi. The mat A-2 and mat A-3 genes, present in the mat A idiomorph of the filamentous fungus Neurospora crassa, are required for post-fertilization functions but are not essential for mating identity. Their putative roles as transcription factors are based on the similarity of mat A-2 with the Podospora anserina SMR1 gene and an HMG motif present in the mat A-3 gene. In this work the yeast two-hybrid system was used to identify transcriptional activity and protein-protein interaction of N. crassa mat A-2 and mat A-3 genes. We observed that the mat A-3 protein alone is capable of weakly activating transcription of yeast reporter genes; it also binds with low specificity to the GAL1 promoter sequence, possibly due to its HMG domain. Our results also indicate that mat A-3 is capable to form homodimers, and interact with mat A-2. Interference on yeast growth was observed on some transformants suggesting a toxic action of the mat A-2 protein. Our data on pattern of interactions of mat proteins contributes towards understanding the control of vegetative and sexual cycles in filamentous fungi.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200023Neurospora crassamating-typeyeast two-hybrid system |
| spellingShingle | Carla C. da Silva Rosana C. Cruz Mônica Bucciarelli-Rodriguez Adlane Vilas-Boas Neurospora crassa mat A-2 and mat A-3 proteins weakly interact in the yeast two-hybrid system and affect yeast growth Genetics and Molecular Biology Neurospora crassa mating-type yeast two-hybrid system |
| title | Neurospora crassa mat A-2 and mat A-3 proteins weakly interact in the yeast two-hybrid system and affect yeast growth |
| title_full | Neurospora crassa mat A-2 and mat A-3 proteins weakly interact in the yeast two-hybrid system and affect yeast growth |
| title_fullStr | Neurospora crassa mat A-2 and mat A-3 proteins weakly interact in the yeast two-hybrid system and affect yeast growth |
| title_full_unstemmed | Neurospora crassa mat A-2 and mat A-3 proteins weakly interact in the yeast two-hybrid system and affect yeast growth |
| title_short | Neurospora crassa mat A-2 and mat A-3 proteins weakly interact in the yeast two-hybrid system and affect yeast growth |
| title_sort | neurospora crassa mat a 2 and mat a 3 proteins weakly interact in the yeast two hybrid system and affect yeast growth |
| topic | Neurospora crassa mating-type yeast two-hybrid system |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200023 |
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