A Natural Glucan from Black Bean Inhibits Cancer Cell Proliferation via PI3K-Akt and MAPK Pathway
A natural α-1,6-glucan named BBWPW was identified from black beans. Cell viability assay showed that BBWPW inhibited the proliferation of different cancer cells, especially HeLa cells. Flow cytometry analysis indicated that BBWPW suppressed the HeLa cell cycle in the G2/M phase. Consistently, RT-PCR...
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MDPI AG
2023-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/4/1971 |
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| author | Peng Li Yihua Hu Lingmin Zhan Jiaqi He Jingwu Lu Chunyan Gao Weijun Du Aiqin Yue Jinzhong Zhao Wuxia Zhang |
| author_facet | Peng Li Yihua Hu Lingmin Zhan Jiaqi He Jingwu Lu Chunyan Gao Weijun Du Aiqin Yue Jinzhong Zhao Wuxia Zhang |
| author_sort | Peng Li |
| collection | DOAJ |
| description | A natural α-1,6-glucan named BBWPW was identified from black beans. Cell viability assay showed that BBWPW inhibited the proliferation of different cancer cells, especially HeLa cells. Flow cytometry analysis indicated that BBWPW suppressed the HeLa cell cycle in the G2/M phase. Consistently, RT-PCR experiments displayed that BBWPW significantly impacts the expression of four marker genes related to the G2/M phase, including <i>p21</i>, <i>CDK1</i>, <i>Cyclin B1</i>, and <i>Survivin</i>. To explore the molecular mechanism of BBWPW to induce cell cycle arrest, a transcriptome-based target inference approach was utilized to predict the potential upstream pathways of BBWPW and it was found that the PI3K-Akt and MAPK signal pathways had the potential to mediate the effects of BBWPW on the cell cycle. Further experimental tests confirmed that BBWPW increased the expression of <i>BAD</i> and <i>AKT</i> and decreased the expression of <i>mTOR</i> and <i>MKK3.</i> These results suggested that BBWPW could regulate the PI3K-Akt and MAPK pathways to induce cell cycle arrest and ultimately inhibit the proliferation of HeLa cells, providing the potential of the black bean glucan to be a natural anticancer drug. |
| first_indexed | 2024-03-11T08:21:03Z |
| format | Article |
| id | doaj.art-6f93eafda55e442c91d26ea4872bf00b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T08:21:03Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6f93eafda55e442c91d26ea4872bf00b2023-11-16T22:25:47ZengMDPI AGMolecules1420-30492023-02-01284197110.3390/molecules28041971A Natural Glucan from Black Bean Inhibits Cancer Cell Proliferation via PI3K-Akt and MAPK PathwayPeng Li0Yihua Hu1Lingmin Zhan2Jiaqi He3Jingwu Lu4Chunyan Gao5Weijun Du6Aiqin Yue7Jinzhong Zhao8Wuxia Zhang9Department of Basic Sciences, Shanxi Agricultural University, Taigu 030801, ChinaDepartment of Basic Sciences, Shanxi Agricultural University, Taigu 030801, ChinaDepartment of Basic Sciences, Shanxi Agricultural University, Taigu 030801, ChinaDepartment of Basic Sciences, Shanxi Agricultural University, Taigu 030801, ChinaDepartment of Basic Sciences, Shanxi Agricultural University, Taigu 030801, ChinaDepartment of Basic Sciences, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Agriculture, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Agriculture, Shanxi Agricultural University, Taigu 030801, ChinaDepartment of Basic Sciences, Shanxi Agricultural University, Taigu 030801, ChinaDepartment of Basic Sciences, Shanxi Agricultural University, Taigu 030801, ChinaA natural α-1,6-glucan named BBWPW was identified from black beans. Cell viability assay showed that BBWPW inhibited the proliferation of different cancer cells, especially HeLa cells. Flow cytometry analysis indicated that BBWPW suppressed the HeLa cell cycle in the G2/M phase. Consistently, RT-PCR experiments displayed that BBWPW significantly impacts the expression of four marker genes related to the G2/M phase, including <i>p21</i>, <i>CDK1</i>, <i>Cyclin B1</i>, and <i>Survivin</i>. To explore the molecular mechanism of BBWPW to induce cell cycle arrest, a transcriptome-based target inference approach was utilized to predict the potential upstream pathways of BBWPW and it was found that the PI3K-Akt and MAPK signal pathways had the potential to mediate the effects of BBWPW on the cell cycle. Further experimental tests confirmed that BBWPW increased the expression of <i>BAD</i> and <i>AKT</i> and decreased the expression of <i>mTOR</i> and <i>MKK3.</i> These results suggested that BBWPW could regulate the PI3K-Akt and MAPK pathways to induce cell cycle arrest and ultimately inhibit the proliferation of HeLa cells, providing the potential of the black bean glucan to be a natural anticancer drug.https://www.mdpi.com/1420-3049/28/4/1971black beanpolysaccharideanti-cancer activitycell cycle arrestPI3K/AktMAPK |
| spellingShingle | Peng Li Yihua Hu Lingmin Zhan Jiaqi He Jingwu Lu Chunyan Gao Weijun Du Aiqin Yue Jinzhong Zhao Wuxia Zhang A Natural Glucan from Black Bean Inhibits Cancer Cell Proliferation via PI3K-Akt and MAPK Pathway Molecules black bean polysaccharide anti-cancer activity cell cycle arrest PI3K/Akt MAPK |
| title | A Natural Glucan from Black Bean Inhibits Cancer Cell Proliferation via PI3K-Akt and MAPK Pathway |
| title_full | A Natural Glucan from Black Bean Inhibits Cancer Cell Proliferation via PI3K-Akt and MAPK Pathway |
| title_fullStr | A Natural Glucan from Black Bean Inhibits Cancer Cell Proliferation via PI3K-Akt and MAPK Pathway |
| title_full_unstemmed | A Natural Glucan from Black Bean Inhibits Cancer Cell Proliferation via PI3K-Akt and MAPK Pathway |
| title_short | A Natural Glucan from Black Bean Inhibits Cancer Cell Proliferation via PI3K-Akt and MAPK Pathway |
| title_sort | natural glucan from black bean inhibits cancer cell proliferation via pi3k akt and mapk pathway |
| topic | black bean polysaccharide anti-cancer activity cell cycle arrest PI3K/Akt MAPK |
| url | https://www.mdpi.com/1420-3049/28/4/1971 |
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