Depression and recovery of IL-17A secretion in mitogen responses in patients with active tuberculosis-a prospective observational study
Background/Purpose: T-helper cell 17 (Th17) is a distinct subset of CD4+ T lymphocytes that is important in the pathogenesis of Mycobacterium tuberculosis infection. This study aims to investigate the characteristics of interleukin (IL)-17A and Th17-related cytokines after stimulation with phytohema...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-04-01
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| Series: | Journal of the Formosan Medical Association |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664620304538 |
| _version_ | 1818440611159605248 |
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| author | Jia-Yih Feng Li-Ing Ho Fan-Yi Chuang Sheng-Wei Pan Ying-Ying Chen Chin-Lien Tung Chiao-Ping Li Wei-Juin Su |
| author_facet | Jia-Yih Feng Li-Ing Ho Fan-Yi Chuang Sheng-Wei Pan Ying-Ying Chen Chin-Lien Tung Chiao-Ping Li Wei-Juin Su |
| author_sort | Jia-Yih Feng |
| collection | DOAJ |
| description | Background/Purpose: T-helper cell 17 (Th17) is a distinct subset of CD4+ T lymphocytes that is important in the pathogenesis of Mycobacterium tuberculosis infection. This study aims to investigate the characteristics of interleukin (IL)-17A and Th17-related cytokines after stimulation with phytohemagglutinin in patients with active tuberculosis (TB). Methods: This prospective cohort study enrolled patients with culture-confirmed active TB. QuantiFERON-TB Gold In-Tube (QFT-GIT) assay was performed upon TB diagnosis and at 2 months after TB treatment. Their non-TB-specific secretion of IL-17A and Th17-related cytokines were measured in supernatants of mitogen tubes in QFT-GIT and compared to those of active TB contacts with or without latent TB infection. We analyzed the association between IL-17A secretions and TB presentation and treatment outcomes. Results: A total of 108 patients with TB and 64 non-TB cases were enrolled. The secretion of IL-17A, IL-21, IL-23, and IL-6 were lower in active TB patients upon TB diagnosis. In active TB patients, lower IL-17A secretions were associated with higher grades of sputum smear. In the multivariate analysis, lower IL-17A secretions served as an independent factor associated with 2-month culture non-conversion (odds ratio 23.04, 95% confidence interval [CI] 1.69–84.78) and on-treatment mortality (hazard ratio 28.54, 95% CI 1.30–99.25). The levels of IL-23, and IL-6 significantly increased after 2 months of anti-TB treatment. Conclusion: The non-TB-specific IL-17A secretions were lower in active TB patients upon TB diagnosis and associated with higher disease severity and worse treatment outcomes. Trend of recovery of the depressed Th17-related cytokines was noted after effective anti-TB treatment. |
| first_indexed | 2024-12-14T18:15:06Z |
| format | Article |
| id | doaj.art-6f9552a6863f4e04bee4879461b4158e |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-12-14T18:15:06Z |
| publishDate | 2021-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-6f9552a6863f4e04bee4879461b4158e2022-12-21T22:52:12ZengElsevierJournal of the Formosan Medical Association0929-66462021-04-01120410801089Depression and recovery of IL-17A secretion in mitogen responses in patients with active tuberculosis-a prospective observational studyJia-Yih Feng0Li-Ing Ho1Fan-Yi Chuang2Sheng-Wei Pan3Ying-Ying Chen4Chin-Lien Tung5Chiao-Ping Li6Wei-Juin Su7Department of Chest Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; School of Medicine, National Yang-Ming University, Taipei, TaiwanDepartment of Chest Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; School of Medicine, National Yang-Ming University, Taipei, TaiwanDepartment of Chest Medicine, Taipei Veterans General Hospital, Taipei, TaiwanDepartment of Chest Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; School of Medicine, National Yang-Ming University, Taipei, Taiwan; Institute of Public Health, National Yang-Ming University, Taipei, TaiwanDepartment of Internal Medicine, Taipei Veterans General Hospital Taoyuan Branch, Taoyuan, TaiwanDepartment of Nursing, Taipei Veterans General Hospital, TaiwanDepartment of Nursing, Taipei Veterans General Hospital, TaiwanDepartment of Chest Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; School of Medicine, National Yang-Ming University, Taipei, Taiwan; Corresponding author. Department of Chest Medicine, Taipei Veterans General Hospital, #201, Section 2, Shih-Pai Road, Taipei 112, Taiwan, ROC. Fax: +886 2 2875 7168.Background/Purpose: T-helper cell 17 (Th17) is a distinct subset of CD4+ T lymphocytes that is important in the pathogenesis of Mycobacterium tuberculosis infection. This study aims to investigate the characteristics of interleukin (IL)-17A and Th17-related cytokines after stimulation with phytohemagglutinin in patients with active tuberculosis (TB). Methods: This prospective cohort study enrolled patients with culture-confirmed active TB. QuantiFERON-TB Gold In-Tube (QFT-GIT) assay was performed upon TB diagnosis and at 2 months after TB treatment. Their non-TB-specific secretion of IL-17A and Th17-related cytokines were measured in supernatants of mitogen tubes in QFT-GIT and compared to those of active TB contacts with or without latent TB infection. We analyzed the association between IL-17A secretions and TB presentation and treatment outcomes. Results: A total of 108 patients with TB and 64 non-TB cases were enrolled. The secretion of IL-17A, IL-21, IL-23, and IL-6 were lower in active TB patients upon TB diagnosis. In active TB patients, lower IL-17A secretions were associated with higher grades of sputum smear. In the multivariate analysis, lower IL-17A secretions served as an independent factor associated with 2-month culture non-conversion (odds ratio 23.04, 95% confidence interval [CI] 1.69–84.78) and on-treatment mortality (hazard ratio 28.54, 95% CI 1.30–99.25). The levels of IL-23, and IL-6 significantly increased after 2 months of anti-TB treatment. Conclusion: The non-TB-specific IL-17A secretions were lower in active TB patients upon TB diagnosis and associated with higher disease severity and worse treatment outcomes. Trend of recovery of the depressed Th17-related cytokines was noted after effective anti-TB treatment.http://www.sciencedirect.com/science/article/pii/S0929664620304538Active tuberculosisMortalitySputum culture conversionTh17 |
| spellingShingle | Jia-Yih Feng Li-Ing Ho Fan-Yi Chuang Sheng-Wei Pan Ying-Ying Chen Chin-Lien Tung Chiao-Ping Li Wei-Juin Su Depression and recovery of IL-17A secretion in mitogen responses in patients with active tuberculosis-a prospective observational study Journal of the Formosan Medical Association Active tuberculosis Mortality Sputum culture conversion Th17 |
| title | Depression and recovery of IL-17A secretion in mitogen responses in patients with active tuberculosis-a prospective observational study |
| title_full | Depression and recovery of IL-17A secretion in mitogen responses in patients with active tuberculosis-a prospective observational study |
| title_fullStr | Depression and recovery of IL-17A secretion in mitogen responses in patients with active tuberculosis-a prospective observational study |
| title_full_unstemmed | Depression and recovery of IL-17A secretion in mitogen responses in patients with active tuberculosis-a prospective observational study |
| title_short | Depression and recovery of IL-17A secretion in mitogen responses in patients with active tuberculosis-a prospective observational study |
| title_sort | depression and recovery of il 17a secretion in mitogen responses in patients with active tuberculosis a prospective observational study |
| topic | Active tuberculosis Mortality Sputum culture conversion Th17 |
| url | http://www.sciencedirect.com/science/article/pii/S0929664620304538 |
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