One-Step Ultrasonic Preparation of Stable Bovine Serum Albumin-Perovskite for Fluorescence Analysis of L-Ascorbic Acid and Alkaline Phosphatase
Halide lead perovskite has attracted increased attention due to its excellent optical properties. However, the poor stability of the halide lead perovskite nanocrystals has been a major obstacle to their application in biosensing. Here, we proposed a method to synthesize CsPbBr<sub>3</sub&g...
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MDPI AG
2023-07-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/8/770 |
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| author | Lei Deng Feng Huang Aomei Zhang Tingting Wang Minghui Yang Xiaoqing Li Xiang Chen |
| author_facet | Lei Deng Feng Huang Aomei Zhang Tingting Wang Minghui Yang Xiaoqing Li Xiang Chen |
| author_sort | Lei Deng |
| collection | DOAJ |
| description | Halide lead perovskite has attracted increased attention due to its excellent optical properties. However, the poor stability of the halide lead perovskite nanocrystals has been a major obstacle to their application in biosensing. Here, we proposed a method to synthesize CsPbBr<sub>3</sub>/BSA NCs perovskite using bovine serum albumin (BSA) as a zwitterion ligand. Then, a fluorescent sensor for alkaline phosphatase determination based on CsPbBr<sub>3</sub>/BSA NCs was successfully built via the interaction of L-ascorbic acid (AA) with BSA on the perovskite surface. Under optimal conditions, the sensor showed a linear concentration range from 50 to 500 μM with a detection limit of 28 μM (signal-to-noise ratio of 3) for AA, and demonstrated a linear concentration range from 40 to 500 U/L with a detection limit of 15.5 U/L (signal-to-noise ratio of 3) for alkaline phosphatase (ALP). In addition, the proposed fluorescent biosensor exhibited good selectivity and recovery in the determination of ALP in human serum. This strategy offers an innovative way for enhancing the water stability of lead halide perovskite and promoting their application in biosensing areas. |
| first_indexed | 2024-03-11T00:05:25Z |
| format | Article |
| id | doaj.art-6f9ae5210fc04888bd7c757262c1fff8 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T00:05:25Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-6f9ae5210fc04888bd7c757262c1fff82023-11-19T00:24:56ZengMDPI AGBiosensors2079-63742023-07-0113877010.3390/bios13080770One-Step Ultrasonic Preparation of Stable Bovine Serum Albumin-Perovskite for Fluorescence Analysis of L-Ascorbic Acid and Alkaline PhosphataseLei Deng0Feng Huang1Aomei Zhang2Tingting Wang3Minghui Yang4Xiaoqing Li5Xiang Chen6Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaHunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaHunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaHunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaHunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaFurong Labratory, Changsha 410083, ChinaFurong Labratory, Changsha 410083, ChinaHalide lead perovskite has attracted increased attention due to its excellent optical properties. However, the poor stability of the halide lead perovskite nanocrystals has been a major obstacle to their application in biosensing. Here, we proposed a method to synthesize CsPbBr<sub>3</sub>/BSA NCs perovskite using bovine serum albumin (BSA) as a zwitterion ligand. Then, a fluorescent sensor for alkaline phosphatase determination based on CsPbBr<sub>3</sub>/BSA NCs was successfully built via the interaction of L-ascorbic acid (AA) with BSA on the perovskite surface. Under optimal conditions, the sensor showed a linear concentration range from 50 to 500 μM with a detection limit of 28 μM (signal-to-noise ratio of 3) for AA, and demonstrated a linear concentration range from 40 to 500 U/L with a detection limit of 15.5 U/L (signal-to-noise ratio of 3) for alkaline phosphatase (ALP). In addition, the proposed fluorescent biosensor exhibited good selectivity and recovery in the determination of ALP in human serum. This strategy offers an innovative way for enhancing the water stability of lead halide perovskite and promoting their application in biosensing areas.https://www.mdpi.com/2079-6374/13/8/770perovskitefluorescence sensingalkaline phosphataseL-ascorbic acid |
| spellingShingle | Lei Deng Feng Huang Aomei Zhang Tingting Wang Minghui Yang Xiaoqing Li Xiang Chen One-Step Ultrasonic Preparation of Stable Bovine Serum Albumin-Perovskite for Fluorescence Analysis of L-Ascorbic Acid and Alkaline Phosphatase Biosensors perovskite fluorescence sensing alkaline phosphatase L-ascorbic acid |
| title | One-Step Ultrasonic Preparation of Stable Bovine Serum Albumin-Perovskite for Fluorescence Analysis of L-Ascorbic Acid and Alkaline Phosphatase |
| title_full | One-Step Ultrasonic Preparation of Stable Bovine Serum Albumin-Perovskite for Fluorescence Analysis of L-Ascorbic Acid and Alkaline Phosphatase |
| title_fullStr | One-Step Ultrasonic Preparation of Stable Bovine Serum Albumin-Perovskite for Fluorescence Analysis of L-Ascorbic Acid and Alkaline Phosphatase |
| title_full_unstemmed | One-Step Ultrasonic Preparation of Stable Bovine Serum Albumin-Perovskite for Fluorescence Analysis of L-Ascorbic Acid and Alkaline Phosphatase |
| title_short | One-Step Ultrasonic Preparation of Stable Bovine Serum Albumin-Perovskite for Fluorescence Analysis of L-Ascorbic Acid and Alkaline Phosphatase |
| title_sort | one step ultrasonic preparation of stable bovine serum albumin perovskite for fluorescence analysis of l ascorbic acid and alkaline phosphatase |
| topic | perovskite fluorescence sensing alkaline phosphatase L-ascorbic acid |
| url | https://www.mdpi.com/2079-6374/13/8/770 |
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