Mass kills in hatchery-reared European seabass (Dicentrarchus labrax) triggered by concomitant infections of Amyloodinium ocellatum and Vibrio alginolyticus
ABSTRACTAmyloodiniosis and vibriosis are serious diseases in European seabass (Dicentrarchus labrax) hatcheries with noticeable high mortality. This study was conducted on tank-cultured D. labrax frys at a private marine hatchery near Mariout Lake (Alexandria, Egypt). Frys showed a high mortality ra...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | International Journal of Veterinary Science and Medicine |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/23144599.2022.2070346 |
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| author | Reham H. Ragab Mamdouh Y. Elgendy Nader M Sabry Mahmoud S. Sharaf Marwa M. Attia Reda M.S. Korany Mohamed Abdelsalam Ahmed S. Eltahan Elsayed A. Eldessouki Ghada O. El-Demerdash Riad H. Khalil Abeer E. Mahmoud Alaa Eldin Eissa |
| author_facet | Reham H. Ragab Mamdouh Y. Elgendy Nader M Sabry Mahmoud S. Sharaf Marwa M. Attia Reda M.S. Korany Mohamed Abdelsalam Ahmed S. Eltahan Elsayed A. Eldessouki Ghada O. El-Demerdash Riad H. Khalil Abeer E. Mahmoud Alaa Eldin Eissa |
| author_sort | Reham H. Ragab |
| collection | DOAJ |
| description | ABSTRACTAmyloodiniosis and vibriosis are serious diseases in European seabass (Dicentrarchus labrax) hatcheries with noticeable high mortality. This study was conducted on tank-cultured D. labrax frys at a private marine hatchery near Mariout Lake (Alexandria, Egypt). Frys showed a high mortality rate (70%), lethargy, darkening, asphyxia, ascites, and velvety skin appearance. Both infectious agents were presumptively identified in all investigated frys. The identities of the two recovered agents were confirmed by molecular assay and phylogenetic analysis. On the tissue level, histopathological examination of skin, splenic, and renal tissue indicated severe alterations due to the direct impacts of both infections. On the cellular level, scanning electron micrographs showed both protozoal and bacterial pathogens on/in gill epithelial cells in solitary and colonial forms. Vibrio alginolyticus showed variable results for tested antibiotics, with a higher sensitivity to florfenicol. A successful control strategy was strictly adopted to overcome infections and stop mortalities. Copper sulphate and hydrogen peroxide were efficiently applied to tank water to overcome A. ocellatum infections. Further, florfenicol was effectively used to overcome systemic V. alginolyticus infections. The efficacy of treatments was confirmed by the absence of infectious agents in randomly collected fish samples. To the best of the authors’ knowledge, this study is one of the earliest Egyptian studies that dealt with the dilemma of mass kills associated with external parasitic/systemic bacterial infections among hatchery-reared European seabass. |
| first_indexed | 2024-04-12T01:17:42Z |
| format | Article |
| id | doaj.art-6f9f5b0d99094e01900148790b62f946 |
| institution | Directory Open Access Journal |
| issn | 2314-4599 |
| language | English |
| last_indexed | 2024-04-12T01:17:42Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Veterinary Science and Medicine |
| spelling | doaj.art-6f9f5b0d99094e01900148790b62f9462022-12-22T03:53:55ZengTaylor & Francis GroupInternational Journal of Veterinary Science and Medicine2314-45992022-12-01101334510.1080/23144599.2022.2070346Mass kills in hatchery-reared European seabass (Dicentrarchus labrax) triggered by concomitant infections of Amyloodinium ocellatum and Vibrio alginolyticusReham H. Ragab0Mamdouh Y. Elgendy1Nader M Sabry2Mahmoud S. Sharaf3Marwa M. Attia4Reda M.S. Korany5Mohamed Abdelsalam6Ahmed S. Eltahan7Elsayed A. Eldessouki8Ghada O. El-Demerdash9Riad H. Khalil10Abeer E. Mahmoud11Alaa Eldin Eissa12Department of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, Cairo University, Giza, EgyptDepartment of Hydrobiology, Veterinary Research Institute, National Research Centre, Giza, EgyptFish Disease Lab, Aquaculture Division, National Institute of Oceanography and Fishery (NIOF), Alexandria, EgyptDepartment of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, Cairo University, Giza, EgyptDepartment of Parasitology, Faculty of Veterinary Medicine, Cairo University, Giza, EgyptDepartment of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, EgyptDepartment of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, Cairo University, Giza, EgyptDepartment of Pathology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, EgyptDepartment of Fish Health and Diseases, Faculty of Fish Resources, Suez University, Suez, EgyptAgriculture Research Centre, Animal Health Research Institute, Al-Fayoum Provincial Laboratory, Giza, EgyptDepartment of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Alexandria University, Alexandria, EgyptDepartment of Fish Diseases, Animal Health Research Institute, Assiut Provincial Laboratory, Agriculture Research Center, Dokki, EgyptDepartment of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, Cairo University, Giza, EgyptABSTRACTAmyloodiniosis and vibriosis are serious diseases in European seabass (Dicentrarchus labrax) hatcheries with noticeable high mortality. This study was conducted on tank-cultured D. labrax frys at a private marine hatchery near Mariout Lake (Alexandria, Egypt). Frys showed a high mortality rate (70%), lethargy, darkening, asphyxia, ascites, and velvety skin appearance. Both infectious agents were presumptively identified in all investigated frys. The identities of the two recovered agents were confirmed by molecular assay and phylogenetic analysis. On the tissue level, histopathological examination of skin, splenic, and renal tissue indicated severe alterations due to the direct impacts of both infections. On the cellular level, scanning electron micrographs showed both protozoal and bacterial pathogens on/in gill epithelial cells in solitary and colonial forms. Vibrio alginolyticus showed variable results for tested antibiotics, with a higher sensitivity to florfenicol. A successful control strategy was strictly adopted to overcome infections and stop mortalities. Copper sulphate and hydrogen peroxide were efficiently applied to tank water to overcome A. ocellatum infections. Further, florfenicol was effectively used to overcome systemic V. alginolyticus infections. The efficacy of treatments was confirmed by the absence of infectious agents in randomly collected fish samples. To the best of the authors’ knowledge, this study is one of the earliest Egyptian studies that dealt with the dilemma of mass kills associated with external parasitic/systemic bacterial infections among hatchery-reared European seabass.https://www.tandfonline.com/doi/10.1080/23144599.2022.2070346Dicentrarchus labraxfrysamyloodiniosisvibriosismolecular characterizationtreatment |
| spellingShingle | Reham H. Ragab Mamdouh Y. Elgendy Nader M Sabry Mahmoud S. Sharaf Marwa M. Attia Reda M.S. Korany Mohamed Abdelsalam Ahmed S. Eltahan Elsayed A. Eldessouki Ghada O. El-Demerdash Riad H. Khalil Abeer E. Mahmoud Alaa Eldin Eissa Mass kills in hatchery-reared European seabass (Dicentrarchus labrax) triggered by concomitant infections of Amyloodinium ocellatum and Vibrio alginolyticus International Journal of Veterinary Science and Medicine Dicentrarchus labrax frys amyloodiniosis vibriosis molecular characterization treatment |
| title | Mass kills in hatchery-reared European seabass (Dicentrarchus labrax) triggered by concomitant infections of Amyloodinium ocellatum and Vibrio alginolyticus |
| title_full | Mass kills in hatchery-reared European seabass (Dicentrarchus labrax) triggered by concomitant infections of Amyloodinium ocellatum and Vibrio alginolyticus |
| title_fullStr | Mass kills in hatchery-reared European seabass (Dicentrarchus labrax) triggered by concomitant infections of Amyloodinium ocellatum and Vibrio alginolyticus |
| title_full_unstemmed | Mass kills in hatchery-reared European seabass (Dicentrarchus labrax) triggered by concomitant infections of Amyloodinium ocellatum and Vibrio alginolyticus |
| title_short | Mass kills in hatchery-reared European seabass (Dicentrarchus labrax) triggered by concomitant infections of Amyloodinium ocellatum and Vibrio alginolyticus |
| title_sort | mass kills in hatchery reared european seabass dicentrarchus labrax triggered by concomitant infections of amyloodinium ocellatum and vibrio alginolyticus |
| topic | Dicentrarchus labrax frys amyloodiniosis vibriosis molecular characterization treatment |
| url | https://www.tandfonline.com/doi/10.1080/23144599.2022.2070346 |
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