Sublethal concentrations of conventional nematicides alter the physiological activities of Meloidogyne incognita and suppress parasitism
Abstract Reducing nematicide dose rates could be a useful strategy for mitigating their negative effects on health and the environment. In this study, enzymatic activities and the parasitic ability of Meloidogyne incognita after exposure to sub-lethal concentrations (0.25, 1, 2, and 5 ppm) of ethopr...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-27270-z |
| _version_ | 1797958644177829888 |
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| author | Abdullah A. Abdel-Rahman Hosny H. Kesba Hoda G. Mohamed Donia F. Kamel Fatma S. Ahmed |
| author_facet | Abdullah A. Abdel-Rahman Hosny H. Kesba Hoda G. Mohamed Donia F. Kamel Fatma S. Ahmed |
| author_sort | Abdullah A. Abdel-Rahman |
| collection | DOAJ |
| description | Abstract Reducing nematicide dose rates could be a useful strategy for mitigating their negative effects on health and the environment. In this study, enzymatic activities and the parasitic ability of Meloidogyne incognita after exposure to sub-lethal concentrations (0.25, 1, 2, and 5 ppm) of ethoprophos, fenamiphos, and oxamyl were investigated. Although the tested concentrations did not show nematicidal properties in vitro, they reduced root galls by at least 30% at 0.25 ppm and up to 67% at 5 ppm in pots, besides disrupting nematode fertility. For all three nematicides at 2 ppm, a chemotaxis assay showed that ≤ 11% of the nematode population was successfully oriented to the host roots, compared to 44% in the control. Ethoprophos and fenamiphos at 5 ppm showed poor inhibitory effects on acetylcholinesterase (AChE) activity (5.6% and 12.5%, respectively). In contrast, the same nematicides were shown to be strong ATPase inhibitors, causing 82.4% and 82.8% inhibition, respectively. At the same concentration, oxamyl moderately inhibited AChE and ATPase-specific activities, the inhibition being 22.5% and 35.2%, respectively. This study suggests that the use of very low nematicide concentrations could be a promising strategy for nematode management. Furthermore, it has also highlighted the role of ATPases as a possible target site for suppressing nematode activity in the development of future nematicides. |
| first_indexed | 2024-04-11T00:22:54Z |
| format | Article |
| id | doaj.art-6fa094aa9d11430ea88dd5cfeb046acc |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T00:22:54Z |
| publishDate | 2023-01-01 |
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| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-6fa094aa9d11430ea88dd5cfeb046acc2023-01-08T12:12:18ZengNature PortfolioScientific Reports2045-23222023-01-011311910.1038/s41598-022-27270-zSublethal concentrations of conventional nematicides alter the physiological activities of Meloidogyne incognita and suppress parasitismAbdullah A. Abdel-Rahman0Hosny H. Kesba1Hoda G. Mohamed2Donia F. Kamel3Fatma S. Ahmed4Zoology and Agricultural Nematology Department, Faculty of Agriculture, Cairo UniversityZoology and Agricultural Nematology Department, Faculty of Agriculture, Cairo UniversityFaculty of Agriculture, Cairo UniversityFaculty of Agriculture, Cairo UniversityDepartment of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo UniversityAbstract Reducing nematicide dose rates could be a useful strategy for mitigating their negative effects on health and the environment. In this study, enzymatic activities and the parasitic ability of Meloidogyne incognita after exposure to sub-lethal concentrations (0.25, 1, 2, and 5 ppm) of ethoprophos, fenamiphos, and oxamyl were investigated. Although the tested concentrations did not show nematicidal properties in vitro, they reduced root galls by at least 30% at 0.25 ppm and up to 67% at 5 ppm in pots, besides disrupting nematode fertility. For all three nematicides at 2 ppm, a chemotaxis assay showed that ≤ 11% of the nematode population was successfully oriented to the host roots, compared to 44% in the control. Ethoprophos and fenamiphos at 5 ppm showed poor inhibitory effects on acetylcholinesterase (AChE) activity (5.6% and 12.5%, respectively). In contrast, the same nematicides were shown to be strong ATPase inhibitors, causing 82.4% and 82.8% inhibition, respectively. At the same concentration, oxamyl moderately inhibited AChE and ATPase-specific activities, the inhibition being 22.5% and 35.2%, respectively. This study suggests that the use of very low nematicide concentrations could be a promising strategy for nematode management. Furthermore, it has also highlighted the role of ATPases as a possible target site for suppressing nematode activity in the development of future nematicides.https://doi.org/10.1038/s41598-022-27270-z |
| spellingShingle | Abdullah A. Abdel-Rahman Hosny H. Kesba Hoda G. Mohamed Donia F. Kamel Fatma S. Ahmed Sublethal concentrations of conventional nematicides alter the physiological activities of Meloidogyne incognita and suppress parasitism Scientific Reports |
| title | Sublethal concentrations of conventional nematicides alter the physiological activities of Meloidogyne incognita and suppress parasitism |
| title_full | Sublethal concentrations of conventional nematicides alter the physiological activities of Meloidogyne incognita and suppress parasitism |
| title_fullStr | Sublethal concentrations of conventional nematicides alter the physiological activities of Meloidogyne incognita and suppress parasitism |
| title_full_unstemmed | Sublethal concentrations of conventional nematicides alter the physiological activities of Meloidogyne incognita and suppress parasitism |
| title_short | Sublethal concentrations of conventional nematicides alter the physiological activities of Meloidogyne incognita and suppress parasitism |
| title_sort | sublethal concentrations of conventional nematicides alter the physiological activities of meloidogyne incognita and suppress parasitism |
| url | https://doi.org/10.1038/s41598-022-27270-z |
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