A Selection of Platforms to Evaluate Surface Adhesion and Biofilm Formation in Controlled Hydrodynamic Conditions
The early colonization of surfaces and subsequent biofilm development have severe impacts in environmental, industrial, and biomedical settings since they entail high costs and health risks. To develop more effective biofilm control strategies, there is a need to obtain laboratory biofilms that rese...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/9/1993 |
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| author | Luciana C. Gomes Filipe J. M. Mergulhão |
| author_facet | Luciana C. Gomes Filipe J. M. Mergulhão |
| author_sort | Luciana C. Gomes |
| collection | DOAJ |
| description | The early colonization of surfaces and subsequent biofilm development have severe impacts in environmental, industrial, and biomedical settings since they entail high costs and health risks. To develop more effective biofilm control strategies, there is a need to obtain laboratory biofilms that resemble those found in natural or man-made settings. Since microbial adhesion and biofilm formation are strongly affected by hydrodynamics, the knowledge of flow characteristics in different marine, food processing, and medical device locations is essential. Once the hydrodynamic conditions are known, platforms for cell adhesion and biofilm formation should be selected and operated, in order to obtain reproducible biofilms that mimic those found in target scenarios. This review focuses on the most widely used platforms that enable the study of initial microbial adhesion and biofilm formation under controlled hydrodynamic conditions—modified Robbins devices, flow chambers, rotating biofilm devices, microplates, and microfluidic devices—and where numerical simulations have been used to define relevant flow characteristics, namely the shear stress and shear rate. |
| first_indexed | 2024-03-10T07:25:09Z |
| format | Article |
| id | doaj.art-6fa72c7711e949048258be87c7c8036f |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T07:25:09Z |
| publishDate | 2021-09-01 |
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| series | Microorganisms |
| spelling | doaj.art-6fa72c7711e949048258be87c7c8036f2023-11-22T14:20:15ZengMDPI AGMicroorganisms2076-26072021-09-0199199310.3390/microorganisms9091993A Selection of Platforms to Evaluate Surface Adhesion and Biofilm Formation in Controlled Hydrodynamic ConditionsLuciana C. Gomes0Filipe J. M. Mergulhão1LEPABE—Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalLEPABE—Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, PortugalThe early colonization of surfaces and subsequent biofilm development have severe impacts in environmental, industrial, and biomedical settings since they entail high costs and health risks. To develop more effective biofilm control strategies, there is a need to obtain laboratory biofilms that resemble those found in natural or man-made settings. Since microbial adhesion and biofilm formation are strongly affected by hydrodynamics, the knowledge of flow characteristics in different marine, food processing, and medical device locations is essential. Once the hydrodynamic conditions are known, platforms for cell adhesion and biofilm formation should be selected and operated, in order to obtain reproducible biofilms that mimic those found in target scenarios. This review focuses on the most widely used platforms that enable the study of initial microbial adhesion and biofilm formation under controlled hydrodynamic conditions—modified Robbins devices, flow chambers, rotating biofilm devices, microplates, and microfluidic devices—and where numerical simulations have been used to define relevant flow characteristics, namely the shear stress and shear rate.https://www.mdpi.com/2076-2607/9/9/1993biofilmmicrobial adhesionhydrodynamicsshear stressshear ratecomputational fluid dynamics |
| spellingShingle | Luciana C. Gomes Filipe J. M. Mergulhão A Selection of Platforms to Evaluate Surface Adhesion and Biofilm Formation in Controlled Hydrodynamic Conditions Microorganisms biofilm microbial adhesion hydrodynamics shear stress shear rate computational fluid dynamics |
| title | A Selection of Platforms to Evaluate Surface Adhesion and Biofilm Formation in Controlled Hydrodynamic Conditions |
| title_full | A Selection of Platforms to Evaluate Surface Adhesion and Biofilm Formation in Controlled Hydrodynamic Conditions |
| title_fullStr | A Selection of Platforms to Evaluate Surface Adhesion and Biofilm Formation in Controlled Hydrodynamic Conditions |
| title_full_unstemmed | A Selection of Platforms to Evaluate Surface Adhesion and Biofilm Formation in Controlled Hydrodynamic Conditions |
| title_short | A Selection of Platforms to Evaluate Surface Adhesion and Biofilm Formation in Controlled Hydrodynamic Conditions |
| title_sort | selection of platforms to evaluate surface adhesion and biofilm formation in controlled hydrodynamic conditions |
| topic | biofilm microbial adhesion hydrodynamics shear stress shear rate computational fluid dynamics |
| url | https://www.mdpi.com/2076-2607/9/9/1993 |
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