Screening of Halophilic Bacteria Able to Degrade Crude Oil Contamination from Alborz Oil Field, Qom, Iran
Halophilic bacteria which adapted to live under extreme conditions are worthy sources of enzymes in industrial microbiology and bioremediation. This study aimed to isolate halophilic bacteria which are able to use crude oil as the only carbon source. Fifteen soil samples were collected from Alborz o...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2017-06-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/screening-of-halophilic-bacteria-able-to-degrade-crude-oil-contamination-fromalborz-oil-field-qom-iran/ |
| _version_ | 1818903061992570880 |
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| author | Maryam Hosseini Javad Fakhari Mushtaq T. Sh. Al-Rubaye Ehsan Ansari Dezfouli |
| author_facet | Maryam Hosseini Javad Fakhari Mushtaq T. Sh. Al-Rubaye Ehsan Ansari Dezfouli |
| author_sort | Maryam Hosseini |
| collection | DOAJ |
| description | Halophilic bacteria which adapted to live under extreme conditions are worthy sources of enzymes in industrial microbiology and bioremediation. This study aimed to isolate halophilic bacteria which are able to use crude oil as the only carbon source. Fifteen soil samples were collected from Alborz oil field near city Qom, Iran. Liquid mediums containing soil samples, 5,10 and 15% of artificial sea water (ASW) and crude oil as the sole carbon source incubated at 37 °C for one week. Two gram negative moderately halophilic bacteria designated BM1 and BM2 were isolated. Supplementary molecular analysis using 16S rDNA sequencing revealed that they belong to Halomonas and Ochrobactrum genera, respectively. Biochemical tests showed they are both positive for extracellular lipase activity. Considering to cleanup the majority of oil contamination at Alborz oil field, further efforts should be focused on research to understand the degradation mechanism and identification of the enzymes involved in this process. |
| first_indexed | 2024-12-19T20:45:34Z |
| format | Article |
| id | doaj.art-6fa7db18e14046f0b9766f573d052e6a |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-19T20:45:34Z |
| publishDate | 2017-06-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-6fa7db18e14046f0b9766f573d052e6a2022-12-21T20:06:17ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2017-06-0111277377810.22207/JPAM.11.2.16Screening of Halophilic Bacteria Able to Degrade Crude Oil Contamination from Alborz Oil Field, Qom, IranMaryam Hosseini0Javad Fakhari1Mushtaq T. Sh. Al-Rubaye2Ehsan Ansari Dezfouli3Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran.Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran.Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran.Dana Gene Pajouh Laboratory, Food and Drug Organization, Ministry of Health and Medical Education, Tehran, Iran.Halophilic bacteria which adapted to live under extreme conditions are worthy sources of enzymes in industrial microbiology and bioremediation. This study aimed to isolate halophilic bacteria which are able to use crude oil as the only carbon source. Fifteen soil samples were collected from Alborz oil field near city Qom, Iran. Liquid mediums containing soil samples, 5,10 and 15% of artificial sea water (ASW) and crude oil as the sole carbon source incubated at 37 °C for one week. Two gram negative moderately halophilic bacteria designated BM1 and BM2 were isolated. Supplementary molecular analysis using 16S rDNA sequencing revealed that they belong to Halomonas and Ochrobactrum genera, respectively. Biochemical tests showed they are both positive for extracellular lipase activity. Considering to cleanup the majority of oil contamination at Alborz oil field, further efforts should be focused on research to understand the degradation mechanism and identification of the enzymes involved in this process.https://microbiologyjournal.org/screening-of-halophilic-bacteria-able-to-degrade-crude-oil-contamination-fromalborz-oil-field-qom-iran/biodegradationcrude oilhalomonasiranochrobactrum |
| spellingShingle | Maryam Hosseini Javad Fakhari Mushtaq T. Sh. Al-Rubaye Ehsan Ansari Dezfouli Screening of Halophilic Bacteria Able to Degrade Crude Oil Contamination from Alborz Oil Field, Qom, Iran Journal of Pure and Applied Microbiology biodegradation crude oil halomonas iran ochrobactrum |
| title | Screening of Halophilic Bacteria Able to Degrade Crude Oil Contamination from Alborz Oil Field, Qom, Iran |
| title_full | Screening of Halophilic Bacteria Able to Degrade Crude Oil Contamination from Alborz Oil Field, Qom, Iran |
| title_fullStr | Screening of Halophilic Bacteria Able to Degrade Crude Oil Contamination from Alborz Oil Field, Qom, Iran |
| title_full_unstemmed | Screening of Halophilic Bacteria Able to Degrade Crude Oil Contamination from Alborz Oil Field, Qom, Iran |
| title_short | Screening of Halophilic Bacteria Able to Degrade Crude Oil Contamination from Alborz Oil Field, Qom, Iran |
| title_sort | screening of halophilic bacteria able to degrade crude oil contamination from alborz oil field qom iran |
| topic | biodegradation crude oil halomonas iran ochrobactrum |
| url | https://microbiologyjournal.org/screening-of-halophilic-bacteria-able-to-degrade-crude-oil-contamination-fromalborz-oil-field-qom-iran/ |
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