Correlation between DNA ploidy, metaphase high-resolution comparative genomic hybridization results and clinical outcome of synovial sarcoma
<p>Abstract</p> <p>Background</p> <p>Although synovial sarcoma is the 3rd most commonly occurring mesenchymal tumor in young adults, usually with a highly aggressive clinical course; remarkable differences can be seen regarding the clinical outcome. According to compara...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-11-01
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| Series: | Diagnostic Pathology |
| Subjects: | |
| Online Access: | http://www.diagnosticpathology.org/content/6/1/107 |
| _version_ | 1828273383238795264 |
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| author | Papp Gergő Fónyad László Pápai Zsuzsanna Antal Imre Szendrői Miklós Szemlaky Zsuzsanna Balogh Zsófia Changchien Yi C Sápi Zoltán |
| author_facet | Papp Gergő Fónyad László Pápai Zsuzsanna Antal Imre Szendrői Miklós Szemlaky Zsuzsanna Balogh Zsófia Changchien Yi C Sápi Zoltán |
| author_sort | Papp Gergő |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Although synovial sarcoma is the 3rd most commonly occurring mesenchymal tumor in young adults, usually with a highly aggressive clinical course; remarkable differences can be seen regarding the clinical outcome. According to comparative genomic hybridization (CGH) data published in the literature, the simple and complex karyotypes show a correlation between the prognosis and clinical outcome. In addition, the connection between DNA ploidy and clinical course is controversial. The aim of this study was using a fine-tuning interpretation of our DNA ploidy results and to compare these with metaphase high-resolution CGH (HR-CGH) results.</p> <p>Methods</p> <p>DNA ploidy was determined on Feulgen-stained smears in 56 synovial sarcoma cases by image cytometry; follow up was available in 46 cases (average: 78 months). In 9 cases HR-CGH analysis was also available.</p> <p>Results</p> <p>10 cases were found DNA-aneuploid, 46 were DNA-diploid by image cytometry. With fine-tuning of the diploid cases according to the 5c exceeding events (single cell aneuploidy), 33 cases were so called "simple-diploid" (without 5c exceeding events) and 13 cases were "complex-diploid"; containing 5c exceeding events (any number). Aneuploid tumors contained large numbers of genetic alterations with the sum gain of at least 2 chromosomes (A-, B- or C-group) detected by HR-CGH. In the "simple-diploid" cases no or few genetic alterations could be detected, whereas the "complex-diploid" samples numerous aberrations (equal or more than 3) could be found.</p> <p>Conclusions</p> <p>Our results show a correlation between the DNA-ploidy, a fine-tuned DNA-ploidy and the HR-CGH results. Furthermore, we found significant correlation between the different ploidy groups and the clinical outcome (p < 0.05).</p> |
| first_indexed | 2024-04-13T06:20:58Z |
| format | Article |
| id | doaj.art-6fb2374004d04f4d964e9803fc760c26 |
| institution | Directory Open Access Journal |
| issn | 1746-1596 |
| language | English |
| last_indexed | 2024-04-13T06:20:58Z |
| publishDate | 2011-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Diagnostic Pathology |
| spelling | doaj.art-6fb2374004d04f4d964e9803fc760c262022-12-22T02:58:39ZengBMCDiagnostic Pathology1746-15962011-11-016110710.1186/1746-1596-6-107Correlation between DNA ploidy, metaphase high-resolution comparative genomic hybridization results and clinical outcome of synovial sarcomaPapp GergőFónyad LászlóPápai ZsuzsannaAntal ImreSzendrői MiklósSzemlaky ZsuzsannaBalogh ZsófiaChangchien Yi CSápi Zoltán<p>Abstract</p> <p>Background</p> <p>Although synovial sarcoma is the 3rd most commonly occurring mesenchymal tumor in young adults, usually with a highly aggressive clinical course; remarkable differences can be seen regarding the clinical outcome. According to comparative genomic hybridization (CGH) data published in the literature, the simple and complex karyotypes show a correlation between the prognosis and clinical outcome. In addition, the connection between DNA ploidy and clinical course is controversial. The aim of this study was using a fine-tuning interpretation of our DNA ploidy results and to compare these with metaphase high-resolution CGH (HR-CGH) results.</p> <p>Methods</p> <p>DNA ploidy was determined on Feulgen-stained smears in 56 synovial sarcoma cases by image cytometry; follow up was available in 46 cases (average: 78 months). In 9 cases HR-CGH analysis was also available.</p> <p>Results</p> <p>10 cases were found DNA-aneuploid, 46 were DNA-diploid by image cytometry. With fine-tuning of the diploid cases according to the 5c exceeding events (single cell aneuploidy), 33 cases were so called "simple-diploid" (without 5c exceeding events) and 13 cases were "complex-diploid"; containing 5c exceeding events (any number). Aneuploid tumors contained large numbers of genetic alterations with the sum gain of at least 2 chromosomes (A-, B- or C-group) detected by HR-CGH. In the "simple-diploid" cases no or few genetic alterations could be detected, whereas the "complex-diploid" samples numerous aberrations (equal or more than 3) could be found.</p> <p>Conclusions</p> <p>Our results show a correlation between the DNA-ploidy, a fine-tuned DNA-ploidy and the HR-CGH results. Furthermore, we found significant correlation between the different ploidy groups and the clinical outcome (p < 0.05).</p>http://www.diagnosticpathology.org/content/6/1/107High-Resolution Comparative Genomic HybridizationHR-CGHsynovial sarcomaDNA ploidyclinical outcomeSYTSSX |
| spellingShingle | Papp Gergő Fónyad László Pápai Zsuzsanna Antal Imre Szendrői Miklós Szemlaky Zsuzsanna Balogh Zsófia Changchien Yi C Sápi Zoltán Correlation between DNA ploidy, metaphase high-resolution comparative genomic hybridization results and clinical outcome of synovial sarcoma Diagnostic Pathology High-Resolution Comparative Genomic Hybridization HR-CGH synovial sarcoma DNA ploidy clinical outcome SYT SSX |
| title | Correlation between DNA ploidy, metaphase high-resolution comparative genomic hybridization results and clinical outcome of synovial sarcoma |
| title_full | Correlation between DNA ploidy, metaphase high-resolution comparative genomic hybridization results and clinical outcome of synovial sarcoma |
| title_fullStr | Correlation between DNA ploidy, metaphase high-resolution comparative genomic hybridization results and clinical outcome of synovial sarcoma |
| title_full_unstemmed | Correlation between DNA ploidy, metaphase high-resolution comparative genomic hybridization results and clinical outcome of synovial sarcoma |
| title_short | Correlation between DNA ploidy, metaphase high-resolution comparative genomic hybridization results and clinical outcome of synovial sarcoma |
| title_sort | correlation between dna ploidy metaphase high resolution comparative genomic hybridization results and clinical outcome of synovial sarcoma |
| topic | High-Resolution Comparative Genomic Hybridization HR-CGH synovial sarcoma DNA ploidy clinical outcome SYT SSX |
| url | http://www.diagnosticpathology.org/content/6/1/107 |
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