Modified staining techniques for the study of Nongeniculate coralline red algae (Rhodophyta)
Different staining techniques of histological slides were tested and modified in order to improve and facilitate the observation of vegetative and reproductive structures that are taxonomically important for genus and species determinations of nongeniculate coralline algae (Corallinaceae, Rhodophyt...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Universidad Autónoma de Baja California
1994-03-01
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| Series: | Ciencias Marinas |
| Subjects: | |
| Online Access: | http://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/978 |
| _version_ | 1797278697845161984 |
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| author | D Fragoso-Tejas J Márquez-Guzmán E Novelo |
| author_facet | D Fragoso-Tejas J Márquez-Guzmán E Novelo |
| author_sort | D Fragoso-Tejas |
| collection | DOAJ |
| description |
Different staining techniques of histological slides were tested and modified in order to improve and facilitate the observation of vegetative and reproductive structures that are taxonomically important for genus and species determinations of nongeniculate coralline algae (Corallinaceae, Rhodophyta). Four groups of staining techniques were differentiated from the results: a) dyes with a low affinity for the thallus: mercuric bromophenol blue, toluidine blue “O” and naphthol blue black; b) dyes with a greater affinity for the thallus: ruthenium red and red “O” oil; c) combined dyes which allowed a better staining of the thallus: naphthol blue black combined with PAS test (Periodic Acid Schiffs reagent) and toluidine blue “O” combined with sodium tetraborate; d) the dyes that gave the best staining results were: Harris’ hematoxylin, Mallory’s phosphotungstic hematoxylin, potassium permanganate (KMnO4). PAS test and safranine-fast green.
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| first_indexed | 2024-03-07T16:12:59Z |
| format | Article |
| id | doaj.art-6fb67979b0e64e3eadb64ada7c963211 |
| institution | Directory Open Access Journal |
| issn | 0185-3880 2395-9053 |
| language | English |
| last_indexed | 2024-03-07T16:12:59Z |
| publishDate | 1994-03-01 |
| publisher | Universidad Autónoma de Baja California |
| record_format | Article |
| series | Ciencias Marinas |
| spelling | doaj.art-6fb67979b0e64e3eadb64ada7c9632112024-03-04T16:43:33ZengUniversidad Autónoma de Baja CaliforniaCiencias Marinas0185-38802395-90531994-03-0120410.7773/cm.v20i4.978Modified staining techniques for the study of Nongeniculate coralline red algae (Rhodophyta)D Fragoso-Tejas0J Márquez-Guzmán1E Novelo2Universidad Nacional Autónoma de MéxicoUniversidad Nacional Autónoma de MéxicoUniversidad Nacional Autónoma de México Different staining techniques of histological slides were tested and modified in order to improve and facilitate the observation of vegetative and reproductive structures that are taxonomically important for genus and species determinations of nongeniculate coralline algae (Corallinaceae, Rhodophyta). Four groups of staining techniques were differentiated from the results: a) dyes with a low affinity for the thallus: mercuric bromophenol blue, toluidine blue “O” and naphthol blue black; b) dyes with a greater affinity for the thallus: ruthenium red and red “O” oil; c) combined dyes which allowed a better staining of the thallus: naphthol blue black combined with PAS test (Periodic Acid Schiffs reagent) and toluidine blue “O” combined with sodium tetraborate; d) the dyes that gave the best staining results were: Harris’ hematoxylin, Mallory’s phosphotungstic hematoxylin, potassium permanganate (KMnO4). PAS test and safranine-fast green. http://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/978Rhodophytanongeniculate coralline red algaestaining techniquesdyes |
| spellingShingle | D Fragoso-Tejas J Márquez-Guzmán E Novelo Modified staining techniques for the study of Nongeniculate coralline red algae (Rhodophyta) Ciencias Marinas Rhodophyta nongeniculate coralline red algae staining techniques dyes |
| title | Modified staining techniques for the study of Nongeniculate coralline red algae (Rhodophyta) |
| title_full | Modified staining techniques for the study of Nongeniculate coralline red algae (Rhodophyta) |
| title_fullStr | Modified staining techniques for the study of Nongeniculate coralline red algae (Rhodophyta) |
| title_full_unstemmed | Modified staining techniques for the study of Nongeniculate coralline red algae (Rhodophyta) |
| title_short | Modified staining techniques for the study of Nongeniculate coralline red algae (Rhodophyta) |
| title_sort | modified staining techniques for the study of nongeniculate coralline red algae rhodophyta |
| topic | Rhodophyta nongeniculate coralline red algae staining techniques dyes |
| url | http://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/978 |
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