Dietary effects of sophorolipids on nutrient bioavailability and intestinal microenvironments in broiler chickens
Using antibiotics as growth promoter has been banned in poultry feed industry, thus various researchers try to seek an alternative to replace the growth-promoting antibiotics. In this study, we aimed to evaluate the growth performance via intestinal nutrient utilization and cecal microbial compositi...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Society of Animal Sciences and Technology
2022-11-01
|
| Series: | Journal of Animal Science and Technology |
| Subjects: | |
| Online Access: | http://www.ejast.org/archive/view_article?doi=10.5187/jast.2022.e76 |
| _version_ | 1811202715461615616 |
|---|---|
| author | Min-Jin Kwak Min Young Park Ki-Peum Sung Hanbae Lee Kwang-Youn Whang Younghoon Kim |
| author_facet | Min-Jin Kwak Min Young Park Ki-Peum Sung Hanbae Lee Kwang-Youn Whang Younghoon Kim |
| author_sort | Min-Jin Kwak |
| collection | DOAJ |
| description | Using antibiotics as growth promoter has been banned in poultry feed industry, thus various researchers try to seek an alternative to replace the growth-promoting antibiotics. In this study, we aimed to evaluate the growth performance via intestinal nutrient utilization and cecal microbial composition of broiler after dietary supplementation with most commonly using antibiotics, zinc bacitracin, and sophorolipid. A total of 180 1-day-old chicks were randomly assigned, and dietary treatment was as follow: CON, basal diet; ZB, 100 ppm of zinc bacitracin supplemented diet; and SPL, 250 ppm of sophorolipid supplemented diet. Their growth performance was evaluated and the samples of blood, small intestine, and ileal and cecal digesta were collected for biochemical, histological, and genomic analyses. The body weight and average daily gain of 7-day-old chicks were higher in ZB and those in overall experimental period were improved by ZB and SPL supplementation (p < 0.05). Their intestinal characteristics were not affected by dietary treatments in duodenum and ileum. Nonetheless, villus height was increased by SPL supplementation in jejunum (p < 0.05). Moreover, dietary SPL supplementation could down-regulate the expression level of pro-inflammatory cytokine, IL-1β (p < 0.05). mRNA levels of lipid and protein transporters did not differ among the treatments, however, relative expression levels of carbohydrate transporters, GLUT2 and SGLT1 were increased in broiler chicken’s jejumum fed zinc bacitracin and sophorolipid supplemented diets (p < 0.05). Dietary zinc bacitracin supplementation could increase the population of Firmicutes in phylum level, and the portion of Turiciacter in genus level. On the other hands, the portion of Faecalibacterium was increased by dietary SPL supplementation compared to the other treatments. Our findings suggest that SPL supplementation improves growth performance through enhanced carbohydrate utilization capacity via improvement of gut morphological status and modulation of the cecal microbial population of broilers. |
| first_indexed | 2024-04-12T02:44:23Z |
| format | Article |
| id | doaj.art-6fbd6ffdde4649d38dc3de3e19216a31 |
| institution | Directory Open Access Journal |
| issn | 2672-0191 2055-0391 |
| language | English |
| last_indexed | 2024-04-12T02:44:23Z |
| publishDate | 2022-11-01 |
| publisher | Korean Society of Animal Sciences and Technology |
| record_format | Article |
| series | Journal of Animal Science and Technology |
| spelling | doaj.art-6fbd6ffdde4649d38dc3de3e19216a312022-12-22T03:51:15ZengKorean Society of Animal Sciences and TechnologyJournal of Animal Science and Technology2672-01912055-03912022-11-016461092110410.5187/jast.2022.e76Dietary effects of sophorolipids on nutrient bioavailability and intestinal microenvironments in broiler chickensMin-Jin Kwak0Min Young Park1Ki-Peum Sung2Hanbae Lee3Kwang-Youn Whang4Younghoon Kim5Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, KoreaDepartment of Basic Science and Craniofacial Biology, New York University College of Dentistry, New York 10012, USAPathway Intermediates, Seoul 06253, KoreaPathway Intermediates, Seoul 06253, KoreaDivision of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul 02841, KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, KoreaUsing antibiotics as growth promoter has been banned in poultry feed industry, thus various researchers try to seek an alternative to replace the growth-promoting antibiotics. In this study, we aimed to evaluate the growth performance via intestinal nutrient utilization and cecal microbial composition of broiler after dietary supplementation with most commonly using antibiotics, zinc bacitracin, and sophorolipid. A total of 180 1-day-old chicks were randomly assigned, and dietary treatment was as follow: CON, basal diet; ZB, 100 ppm of zinc bacitracin supplemented diet; and SPL, 250 ppm of sophorolipid supplemented diet. Their growth performance was evaluated and the samples of blood, small intestine, and ileal and cecal digesta were collected for biochemical, histological, and genomic analyses. The body weight and average daily gain of 7-day-old chicks were higher in ZB and those in overall experimental period were improved by ZB and SPL supplementation (p < 0.05). Their intestinal characteristics were not affected by dietary treatments in duodenum and ileum. Nonetheless, villus height was increased by SPL supplementation in jejunum (p < 0.05). Moreover, dietary SPL supplementation could down-regulate the expression level of pro-inflammatory cytokine, IL-1β (p < 0.05). mRNA levels of lipid and protein transporters did not differ among the treatments, however, relative expression levels of carbohydrate transporters, GLUT2 and SGLT1 were increased in broiler chicken’s jejumum fed zinc bacitracin and sophorolipid supplemented diets (p < 0.05). Dietary zinc bacitracin supplementation could increase the population of Firmicutes in phylum level, and the portion of Turiciacter in genus level. On the other hands, the portion of Faecalibacterium was increased by dietary SPL supplementation compared to the other treatments. Our findings suggest that SPL supplementation improves growth performance through enhanced carbohydrate utilization capacity via improvement of gut morphological status and modulation of the cecal microbial population of broilers. http://www.ejast.org/archive/view_article?doi=10.5187/jast.2022.e76BroilerGrowthGut microenvironmentNutrient transporterSophorolipidZinc bacitracin |
| spellingShingle | Min-Jin Kwak Min Young Park Ki-Peum Sung Hanbae Lee Kwang-Youn Whang Younghoon Kim Dietary effects of sophorolipids on nutrient bioavailability and intestinal microenvironments in broiler chickens Journal of Animal Science and Technology Broiler Growth Gut microenvironment Nutrient transporter Sophorolipid Zinc bacitracin |
| title | Dietary effects of sophorolipids on nutrient bioavailability and intestinal microenvironments in broiler chickens |
| title_full | Dietary effects of sophorolipids on nutrient bioavailability and intestinal microenvironments in broiler chickens |
| title_fullStr | Dietary effects of sophorolipids on nutrient bioavailability and intestinal microenvironments in broiler chickens |
| title_full_unstemmed | Dietary effects of sophorolipids on nutrient bioavailability and intestinal microenvironments in broiler chickens |
| title_short | Dietary effects of sophorolipids on nutrient bioavailability and intestinal microenvironments in broiler chickens |
| title_sort | dietary effects of sophorolipids on nutrient bioavailability and intestinal microenvironments in broiler chickens |
| topic | Broiler Growth Gut microenvironment Nutrient transporter Sophorolipid Zinc bacitracin |
| url | http://www.ejast.org/archive/view_article?doi=10.5187/jast.2022.e76 |
| work_keys_str_mv | AT minjinkwak dietaryeffectsofsophorolipidsonnutrientbioavailabilityandintestinalmicroenvironmentsinbroilerchickens AT minyoungpark dietaryeffectsofsophorolipidsonnutrientbioavailabilityandintestinalmicroenvironmentsinbroilerchickens AT kipeumsung dietaryeffectsofsophorolipidsonnutrientbioavailabilityandintestinalmicroenvironmentsinbroilerchickens AT hanbaelee dietaryeffectsofsophorolipidsonnutrientbioavailabilityandintestinalmicroenvironmentsinbroilerchickens AT kwangyounwhang dietaryeffectsofsophorolipidsonnutrientbioavailabilityandintestinalmicroenvironmentsinbroilerchickens AT younghoonkim dietaryeffectsofsophorolipidsonnutrientbioavailabilityandintestinalmicroenvironmentsinbroilerchickens |