Therapeutic Potential of Quercetin Loaded Nanoparticles: Novel Insights in Alleviating Colitis in an Experimental DSS Induced Colitis Model
Oxidative stress is considered the main etiologic factor involved in inflammatory bowel disease (IBD). Integration of nanocarriers for natural therapeutic agents with antioxidant and anti-inflammatory potential is a novel promising candidate for curing IBD. Herein, the colonic antioxidant and anti-i...
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MDPI AG
2022-07-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/7/1654 |
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| author | Safaa I. Khater Marwa M. Lotfy Maher N. Alandiyjany Leena S. Alqahtani Asmaa W. Zaglool Fayez Althobaiti Tamer Ahmed Ismail Mohamed Mohamed Soliman Saydat Saad Doaa Ibrahim |
| author_facet | Safaa I. Khater Marwa M. Lotfy Maher N. Alandiyjany Leena S. Alqahtani Asmaa W. Zaglool Fayez Althobaiti Tamer Ahmed Ismail Mohamed Mohamed Soliman Saydat Saad Doaa Ibrahim |
| author_sort | Safaa I. Khater |
| collection | DOAJ |
| description | Oxidative stress is considered the main etiologic factor involved in inflammatory bowel disease (IBD). Integration of nanocarriers for natural therapeutic agents with antioxidant and anti-inflammatory potential is a novel promising candidate for curing IBD. Herein, the colonic antioxidant and anti-inflammatory effects of different concentrations of quercetin nanoparticles (QT-NPs) were evaluated using a dextran sulfate sodium (DSS)-induced colitis model. Following colitis induction, the efficacy and mechanistic actions of QT-NPs were evaluated by assessing lesion severity, molecular aids controlling oxidative stress and inflammatory response, and histopathological and immunohistochemistry examination of colonic tissues. Administration of QT-NPs, especially at higher concentrations, significantly reduced the disease activity index and values of fecal calprotectin marker compared to the colitic group. Colonic oxidant/antioxidant status (ROS, H<sub>2</sub>O<sub>2</sub>, MDA, SOD, CAT, GPX and TAC) was restored after treatment with higher concentrations of QT-NPs. Moreover, QT-NPs at levels of 20 mg/kg and, to a lesser extent, 15 mg/kg reduced Nrf2 and HO-1 gene expression, which was in line with decreasing the expression of iNOS and COX2 in colonic tissues. Higher concentrations of QT-NPs greatly downregulated pro-inflammatory cytokines; upregulated genes encoding occludin, MUC-2 and JAM; and restored the healthy architectures of colonic tissues. Taken together, these data suggest that QT-NPs could be a promising alternative to current IBD treatments. |
| first_indexed | 2024-03-09T10:22:16Z |
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| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T10:22:16Z |
| publishDate | 2022-07-01 |
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| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-6fbe46846ccc43b1991a63b8d6c49d072023-12-01T21:56:11ZengMDPI AGBiomedicines2227-90592022-07-01107165410.3390/biomedicines10071654Therapeutic Potential of Quercetin Loaded Nanoparticles: Novel Insights in Alleviating Colitis in an Experimental DSS Induced Colitis ModelSafaa I. Khater0Marwa M. Lotfy1Maher N. Alandiyjany2Leena S. Alqahtani3Asmaa W. Zaglool4Fayez Althobaiti5Tamer Ahmed Ismail6Mohamed Mohamed Soliman7Saydat Saad8Doaa Ibrahim9Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, EgyptFaculty of Pharmacy, Zagazig University, Zagazig 44511, EgyptLaboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah 21955, Saudi ArabiaDepartment of Biochemistry, College of Science, University of Jeddah, Jeddah 80203, Saudi ArabiaDepartment of Animal Wealth Development, Genetic and Genetic Engineering, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, EgyptDepartment of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Clinical Laboratory Sciences, Turabah University College, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Clinical Laboratory Sciences, Turabah University College, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, EgyptDepartment of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, EgyptOxidative stress is considered the main etiologic factor involved in inflammatory bowel disease (IBD). Integration of nanocarriers for natural therapeutic agents with antioxidant and anti-inflammatory potential is a novel promising candidate for curing IBD. Herein, the colonic antioxidant and anti-inflammatory effects of different concentrations of quercetin nanoparticles (QT-NPs) were evaluated using a dextran sulfate sodium (DSS)-induced colitis model. Following colitis induction, the efficacy and mechanistic actions of QT-NPs were evaluated by assessing lesion severity, molecular aids controlling oxidative stress and inflammatory response, and histopathological and immunohistochemistry examination of colonic tissues. Administration of QT-NPs, especially at higher concentrations, significantly reduced the disease activity index and values of fecal calprotectin marker compared to the colitic group. Colonic oxidant/antioxidant status (ROS, H<sub>2</sub>O<sub>2</sub>, MDA, SOD, CAT, GPX and TAC) was restored after treatment with higher concentrations of QT-NPs. Moreover, QT-NPs at levels of 20 mg/kg and, to a lesser extent, 15 mg/kg reduced Nrf2 and HO-1 gene expression, which was in line with decreasing the expression of iNOS and COX2 in colonic tissues. Higher concentrations of QT-NPs greatly downregulated pro-inflammatory cytokines; upregulated genes encoding occludin, MUC-2 and JAM; and restored the healthy architectures of colonic tissues. Taken together, these data suggest that QT-NPs could be a promising alternative to current IBD treatments.https://www.mdpi.com/2227-9059/10/7/1654nano-therapyquercetin-loaded nanoparticlescolitisoxidative stressinflammatory markersimmunohistochemistry |
| spellingShingle | Safaa I. Khater Marwa M. Lotfy Maher N. Alandiyjany Leena S. Alqahtani Asmaa W. Zaglool Fayez Althobaiti Tamer Ahmed Ismail Mohamed Mohamed Soliman Saydat Saad Doaa Ibrahim Therapeutic Potential of Quercetin Loaded Nanoparticles: Novel Insights in Alleviating Colitis in an Experimental DSS Induced Colitis Model Biomedicines nano-therapy quercetin-loaded nanoparticles colitis oxidative stress inflammatory markers immunohistochemistry |
| title | Therapeutic Potential of Quercetin Loaded Nanoparticles: Novel Insights in Alleviating Colitis in an Experimental DSS Induced Colitis Model |
| title_full | Therapeutic Potential of Quercetin Loaded Nanoparticles: Novel Insights in Alleviating Colitis in an Experimental DSS Induced Colitis Model |
| title_fullStr | Therapeutic Potential of Quercetin Loaded Nanoparticles: Novel Insights in Alleviating Colitis in an Experimental DSS Induced Colitis Model |
| title_full_unstemmed | Therapeutic Potential of Quercetin Loaded Nanoparticles: Novel Insights in Alleviating Colitis in an Experimental DSS Induced Colitis Model |
| title_short | Therapeutic Potential of Quercetin Loaded Nanoparticles: Novel Insights in Alleviating Colitis in an Experimental DSS Induced Colitis Model |
| title_sort | therapeutic potential of quercetin loaded nanoparticles novel insights in alleviating colitis in an experimental dss induced colitis model |
| topic | nano-therapy quercetin-loaded nanoparticles colitis oxidative stress inflammatory markers immunohistochemistry |
| url | https://www.mdpi.com/2227-9059/10/7/1654 |
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