Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids
ABSTRACT The development of efficient and low-cost genotyping methods is essential to precise genetic characterization of cultivars. Here, we present a system based on fluorescently labeled universal tail sequence primers (UTSP) to resolve microsatellite (SSR) markers as an alternative for molecular...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
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| Series: | Scientia Agricola |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000200163&lng=en&tlng=en |
| _version_ | 1828157771029151744 |
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| author | Carlos Alexandre Gomes Ribeiro Marcos de Oliveira Pinto Talles Eduardo Ferreira Maciel Maria Marta Pastina Everaldo Gonçalves de Barros Claudia Teixeira Guimarães |
| author_facet | Carlos Alexandre Gomes Ribeiro Marcos de Oliveira Pinto Talles Eduardo Ferreira Maciel Maria Marta Pastina Everaldo Gonçalves de Barros Claudia Teixeira Guimarães |
| author_sort | Carlos Alexandre Gomes Ribeiro |
| collection | DOAJ |
| description | ABSTRACT The development of efficient and low-cost genotyping methods is essential to precise genetic characterization of cultivars. Here, we present a system based on fluorescently labeled universal tail sequence primers (UTSP) to resolve microsatellite (SSR) markers as an alternative for molecular fingerprinting of maize. A set of 20 SSRs using the UTSP presented an average polymorphic information content of 0.84, which provided a probability of random identity ranging from 10−7 to 10−14, and a minimum exclusion power of 99.99998 % in a group of 48 tropical maize single-cross hybrids traded in Brazil. The genetic diversity analysis based on multidimensional scaling explained approximately 28 % of the total variance for the first two coordinates, tending to group the hybrids according to their respective origin. Additionally, this genotyping system presented a high distinctiveness capacity, which is widely recommended for genetic purity and fingerprinting analyses. Thus, this marker system has a strong potential as a tool for complementary analysis of distinguishability, uniformity and stability required for cultivar registration. |
| first_indexed | 2024-04-11T23:34:33Z |
| format | Article |
| id | doaj.art-6fc4e54d0fb546bb9d68834d38025552 |
| institution | Directory Open Access Journal |
| issn | 1678-992X |
| language | English |
| last_indexed | 2024-04-11T23:34:33Z |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Scientia Agricola |
| spelling | doaj.art-6fc4e54d0fb546bb9d68834d380255522022-12-22T03:56:59ZengUniversidade de São PauloScientia Agricola1678-992X74216316810.1590/1678-992x-2016-0079S0103-90162017000200163Universal tail sequence-SSR applied to molecular characterization of tropical maize hybridsCarlos Alexandre Gomes RibeiroMarcos de Oliveira PintoTalles Eduardo Ferreira MacielMaria Marta PastinaEveraldo Gonçalves de BarrosClaudia Teixeira GuimarãesABSTRACT The development of efficient and low-cost genotyping methods is essential to precise genetic characterization of cultivars. Here, we present a system based on fluorescently labeled universal tail sequence primers (UTSP) to resolve microsatellite (SSR) markers as an alternative for molecular fingerprinting of maize. A set of 20 SSRs using the UTSP presented an average polymorphic information content of 0.84, which provided a probability of random identity ranging from 10−7 to 10−14, and a minimum exclusion power of 99.99998 % in a group of 48 tropical maize single-cross hybrids traded in Brazil. The genetic diversity analysis based on multidimensional scaling explained approximately 28 % of the total variance for the first two coordinates, tending to group the hybrids according to their respective origin. Additionally, this genotyping system presented a high distinctiveness capacity, which is widely recommended for genetic purity and fingerprinting analyses. Thus, this marker system has a strong potential as a tool for complementary analysis of distinguishability, uniformity and stability required for cultivar registration.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000200163&lng=en&tlng=enZea maysfingerprintinggenotypingcultivar protection |
| spellingShingle | Carlos Alexandre Gomes Ribeiro Marcos de Oliveira Pinto Talles Eduardo Ferreira Maciel Maria Marta Pastina Everaldo Gonçalves de Barros Claudia Teixeira Guimarães Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids Scientia Agricola Zea mays fingerprinting genotyping cultivar protection |
| title | Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids |
| title_full | Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids |
| title_fullStr | Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids |
| title_full_unstemmed | Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids |
| title_short | Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids |
| title_sort | universal tail sequence ssr applied to molecular characterization of tropical maize hybrids |
| topic | Zea mays fingerprinting genotyping cultivar protection |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000200163&lng=en&tlng=en |
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