<it>In vitro </it>antibacterial activity and acute toxicity studies of aqueous-methanol extract of <it>Sida rhombifolia </it>Linn. (Malvaceae)
<p>Abstract</p> <p>Background</p> <p>Many bacteria among the Enterobacteria family are involved in infectious diseases and diarrhoea. Most of these bacteria become resistant to the most commonly used synthetic drugs in Cameroon. Natural substances seem to be an alternat...
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| Format: | Article |
| Language: | English |
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BMC
2010-07-01
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| Series: | BMC Complementary and Alternative Medicine |
| Online Access: | http://www.biomedcentral.com/1472-6882/10/40 |
| _version_ | 1818083038091804672 |
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| author | Pieme CA Dzoyem JP Assam JP Assam Penlap VB |
| author_facet | Pieme CA Dzoyem JP Assam JP Assam Penlap VB |
| author_sort | Pieme CA |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Many bacteria among the Enterobacteria family are involved in infectious diseases and diarrhoea. Most of these bacteria become resistant to the most commonly used synthetic drugs in Cameroon. Natural substances seem to be an alternative to this problem. Thus the aim of this research was to investigate the <it>in vitro </it>antibacterial activity of the methanol and aqueous-methanol extracts of <it>Sida rhombifolia </it>Linn (Malvaceae) against seven pathogenic bacteria involved in diarrhoea. Acute toxicity of the most active extract was determined and major bioactive components were screened.</p> <p>Methods</p> <p>The agar disc diffusion and the agar dilution method were used for the determination of inhibition diameters and the Minimum Inhibitory Concentration (MICs) respectively. The acute toxicity study was performed according WHO protocol.</p> <p>Results</p> <p>The aqueous-methanol extract (1v:4v) was the most active with diameters of inhibition zones ranging from 8.7 - 23.6 mm, however at 200 μg/dic this activity was relatively weak compared to gentamycin. The MICs of the aqueous-methanol extract (1v:4v) varied from 49.40 to 78.30 μg/ml. <it>Salmonella dysenteriae </it>was the most sensitive (49.40 μg/ml). For the acute toxicity study, no deaths of rats were recorded. However, significant increase of some biochemical parameters such as aspartate amino-transferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and creatinine (CRT) were found. The phytochemical analysis of the aqueous methanol extract indicated the presence of tannins, polyphenols, alkaloids, glycosides, flavonoids and saponins</p> <p>Conclusion</p> <p>The results showed that the aqueous-methanol extract of <it>S. rhombifolia </it>exhibited moderate antibacterial activity. Some toxic effects were found when rats received more than 8 g/kg bw of extract.</p> <p><it>Antibacterial; Enterobacteria; Acute toxicity; Phytochemical analysis</it></p> |
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| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-10T19:31:38Z |
| publishDate | 2010-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-6fc6fd9e0ba04a17999dd7b9cb0a35a32022-12-22T01:36:14ZengBMCBMC Complementary and Alternative Medicine1472-68822010-07-011014010.1186/1472-6882-10-40<it>In vitro </it>antibacterial activity and acute toxicity studies of aqueous-methanol extract of <it>Sida rhombifolia </it>Linn. (Malvaceae)Pieme CADzoyem JPAssam JP AssamPenlap VB<p>Abstract</p> <p>Background</p> <p>Many bacteria among the Enterobacteria family are involved in infectious diseases and diarrhoea. Most of these bacteria become resistant to the most commonly used synthetic drugs in Cameroon. Natural substances seem to be an alternative to this problem. Thus the aim of this research was to investigate the <it>in vitro </it>antibacterial activity of the methanol and aqueous-methanol extracts of <it>Sida rhombifolia </it>Linn (Malvaceae) against seven pathogenic bacteria involved in diarrhoea. Acute toxicity of the most active extract was determined and major bioactive components were screened.</p> <p>Methods</p> <p>The agar disc diffusion and the agar dilution method were used for the determination of inhibition diameters and the Minimum Inhibitory Concentration (MICs) respectively. The acute toxicity study was performed according WHO protocol.</p> <p>Results</p> <p>The aqueous-methanol extract (1v:4v) was the most active with diameters of inhibition zones ranging from 8.7 - 23.6 mm, however at 200 μg/dic this activity was relatively weak compared to gentamycin. The MICs of the aqueous-methanol extract (1v:4v) varied from 49.40 to 78.30 μg/ml. <it>Salmonella dysenteriae </it>was the most sensitive (49.40 μg/ml). For the acute toxicity study, no deaths of rats were recorded. However, significant increase of some biochemical parameters such as aspartate amino-transferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and creatinine (CRT) were found. The phytochemical analysis of the aqueous methanol extract indicated the presence of tannins, polyphenols, alkaloids, glycosides, flavonoids and saponins</p> <p>Conclusion</p> <p>The results showed that the aqueous-methanol extract of <it>S. rhombifolia </it>exhibited moderate antibacterial activity. Some toxic effects were found when rats received more than 8 g/kg bw of extract.</p> <p><it>Antibacterial; Enterobacteria; Acute toxicity; Phytochemical analysis</it></p>http://www.biomedcentral.com/1472-6882/10/40 |
| spellingShingle | Pieme CA Dzoyem JP Assam JP Assam Penlap VB <it>In vitro </it>antibacterial activity and acute toxicity studies of aqueous-methanol extract of <it>Sida rhombifolia </it>Linn. (Malvaceae) BMC Complementary and Alternative Medicine |
| title | <it>In vitro </it>antibacterial activity and acute toxicity studies of aqueous-methanol extract of <it>Sida rhombifolia </it>Linn. (Malvaceae) |
| title_full | <it>In vitro </it>antibacterial activity and acute toxicity studies of aqueous-methanol extract of <it>Sida rhombifolia </it>Linn. (Malvaceae) |
| title_fullStr | <it>In vitro </it>antibacterial activity and acute toxicity studies of aqueous-methanol extract of <it>Sida rhombifolia </it>Linn. (Malvaceae) |
| title_full_unstemmed | <it>In vitro </it>antibacterial activity and acute toxicity studies of aqueous-methanol extract of <it>Sida rhombifolia </it>Linn. (Malvaceae) |
| title_short | <it>In vitro </it>antibacterial activity and acute toxicity studies of aqueous-methanol extract of <it>Sida rhombifolia </it>Linn. (Malvaceae) |
| title_sort | it in vitro it antibacterial activity and acute toxicity studies of aqueous methanol extract of it sida rhombifolia it linn malvaceae |
| url | http://www.biomedcentral.com/1472-6882/10/40 |
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