Administration of artinm lectin reduces the severity of the acute phase infection with Trypanosoma cruzi
Abstract The acute phase of experimental Trypanosoma cruzi infection is associated with a strong inflammatory reaction, physiological changes, amastigote nests in tissues, and hematological alterations. ArtinM, a lectin extracted from Artocarpus heterophyllus seeds, is a homotetramer exhibiting immu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-05-01
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| Series: | FASEB BioAdvances |
| Subjects: | |
| Online Access: | https://doi.org/10.1096/fba.2020-00065 |
| _version_ | 1818736536932319232 |
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| author | Camila Botelho Miguel Thiago Aparecido da silva Wellington Francisco Rodrigues Patrícia Kellen Martins Oliveira‐Brito Maria Cristina Roque‐Barreira Javier Emílio Lazo‐Chica |
| author_facet | Camila Botelho Miguel Thiago Aparecido da silva Wellington Francisco Rodrigues Patrícia Kellen Martins Oliveira‐Brito Maria Cristina Roque‐Barreira Javier Emílio Lazo‐Chica |
| author_sort | Camila Botelho Miguel |
| collection | DOAJ |
| description | Abstract The acute phase of experimental Trypanosoma cruzi infection is associated with a strong inflammatory reaction, physiological changes, amastigote nests in tissues, and hematological alterations. ArtinM, a lectin extracted from Artocarpus heterophyllus seeds, is a homotetramer exhibiting immunomodulatory properties that promotes Th1 immune responses against intracellular pathogens, including the induction of neutrophil migration and increase in IL‐12 production. This study aimed to evaluate the effects of ArtinM on experimental Chagas disease in mice. We evaluated mouse survival curves, parasitemia, hematological parameters including quantification of inflammatory infiltrates, and amastigote nests in cardiac tissue during infection. The results showed a reduced number of parasites in the blood, an increase in animal survival, improvements in hematological parameters, and decrease in inflammatory infiltrates and amastigote nests in the group treated with ArtinM. Collectively, these data suggest that the administration of ArtinM can lower the number of parasites in peak parasitemia caused by the Colombian strain of T. cruzi and can increase survival of infected mice. The observed reduction in cardiac tissue injury may be due to fewer T. cruzi amastigote nests and lower levels of inflammatory infiltrates. This study highlights the need for further investigation into the use of ArtinM as a potential alternative therapeutic for treating Chagas disease. |
| first_indexed | 2024-12-18T00:38:43Z |
| format | Article |
| id | doaj.art-6fcb5f2adcb447db82976f5ce03e557d |
| institution | Directory Open Access Journal |
| issn | 2573-9832 |
| language | English |
| last_indexed | 2024-12-18T00:38:43Z |
| publishDate | 2021-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | FASEB BioAdvances |
| spelling | doaj.art-6fcb5f2adcb447db82976f5ce03e557d2022-12-21T21:26:57ZengWileyFASEB BioAdvances2573-98322021-05-013529530410.1096/fba.2020-00065Administration of artinm lectin reduces the severity of the acute phase infection with Trypanosoma cruziCamila Botelho Miguel0Thiago Aparecido da silva1Wellington Francisco Rodrigues2Patrícia Kellen Martins Oliveira‐Brito3Maria Cristina Roque‐Barreira4Javier Emílio Lazo‐Chica5Postgraduate course in Health Sciences Federal University of Triangulo Mineiro Uberaba Minas Gerais BrazilDepartment of Cellular and Molecular Biology and Pathogenic Bioagents Ribeirão Preto Medical School University of São Paulo Ribeirão Preto São Paulo BrazilPostgraduate course in Health Sciences Federal University of Triangulo Mineiro Uberaba Minas Gerais BrazilDepartment of Cellular and Molecular Biology and Pathogenic Bioagents Ribeirão Preto Medical School University of São Paulo Ribeirão Preto São Paulo BrazilDepartment of Cellular and Molecular Biology and Pathogenic Bioagents Ribeirão Preto Medical School University of São Paulo Ribeirão Preto São Paulo BrazilPostgraduate course in Health Sciences Federal University of Triangulo Mineiro Uberaba Minas Gerais BrazilAbstract The acute phase of experimental Trypanosoma cruzi infection is associated with a strong inflammatory reaction, physiological changes, amastigote nests in tissues, and hematological alterations. ArtinM, a lectin extracted from Artocarpus heterophyllus seeds, is a homotetramer exhibiting immunomodulatory properties that promotes Th1 immune responses against intracellular pathogens, including the induction of neutrophil migration and increase in IL‐12 production. This study aimed to evaluate the effects of ArtinM on experimental Chagas disease in mice. We evaluated mouse survival curves, parasitemia, hematological parameters including quantification of inflammatory infiltrates, and amastigote nests in cardiac tissue during infection. The results showed a reduced number of parasites in the blood, an increase in animal survival, improvements in hematological parameters, and decrease in inflammatory infiltrates and amastigote nests in the group treated with ArtinM. Collectively, these data suggest that the administration of ArtinM can lower the number of parasites in peak parasitemia caused by the Colombian strain of T. cruzi and can increase survival of infected mice. The observed reduction in cardiac tissue injury may be due to fewer T. cruzi amastigote nests and lower levels of inflammatory infiltrates. This study highlights the need for further investigation into the use of ArtinM as a potential alternative therapeutic for treating Chagas disease.https://doi.org/10.1096/fba.2020-00065ArtinMChagas diseaseparasitemiaTrypanosoma cruzi |
| spellingShingle | Camila Botelho Miguel Thiago Aparecido da silva Wellington Francisco Rodrigues Patrícia Kellen Martins Oliveira‐Brito Maria Cristina Roque‐Barreira Javier Emílio Lazo‐Chica Administration of artinm lectin reduces the severity of the acute phase infection with Trypanosoma cruzi FASEB BioAdvances ArtinM Chagas disease parasitemia Trypanosoma cruzi |
| title | Administration of artinm lectin reduces the severity of the acute phase infection with Trypanosoma cruzi |
| title_full | Administration of artinm lectin reduces the severity of the acute phase infection with Trypanosoma cruzi |
| title_fullStr | Administration of artinm lectin reduces the severity of the acute phase infection with Trypanosoma cruzi |
| title_full_unstemmed | Administration of artinm lectin reduces the severity of the acute phase infection with Trypanosoma cruzi |
| title_short | Administration of artinm lectin reduces the severity of the acute phase infection with Trypanosoma cruzi |
| title_sort | administration of artinm lectin reduces the severity of the acute phase infection with trypanosoma cruzi |
| topic | ArtinM Chagas disease parasitemia Trypanosoma cruzi |
| url | https://doi.org/10.1096/fba.2020-00065 |
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