Non-thermal plasma radiation-induced changes in antibiotic susceptibility and protein profile of Staphylococcus aureus
Background and Objectives: Plasma radiation is a widely used technique for sterilization or decontamination in various industries, as well as in some healthcare settings such as dentistry. The primary aim of this study was to assess the potential of plasma radiation to create a new population of St...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2023-08-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/3814 |
| _version_ | 1797688897231126528 |
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| author | Mehrdad Rabbani-Esfahani Lida Ghaderi Parisa Shali Alireza Ghassempour Seyed Iman Hosseini Atousa Aliahmadi |
| author_facet | Mehrdad Rabbani-Esfahani Lida Ghaderi Parisa Shali Alireza Ghassempour Seyed Iman Hosseini Atousa Aliahmadi |
| author_sort | Mehrdad Rabbani-Esfahani |
| collection | DOAJ |
| description |
Background and Objectives: Plasma radiation is a widely used technique for sterilization or decontamination in various industries, as well as in some healthcare settings such as dentistry. The primary aim of this study was to assess the potential of plasma radiation to create a new population of Staphylococcus aureus cells with distinct characteristics that could lead to novel healthcare challenges.
Materials and Methods: A homemade non-thermal plasma apparatus was applied and the effects of plasma treatment on S. aureus ATCC25923 was assessed. Plasma radiation was applied under controlled conditions to ensure that some bacterial cells remained viable. The treatment was repeated 10 times, with each round followed by a recovery phase to collect any surviving bacterial cells. To assess the potential changes in the bacterial population, we examined the antibiotic susceptibility pattern, micro-structural characteristics using scanning electron microscopy (SEM), and total protein profile using the matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) technique.
Results: The experimental results revealed slight variations in the antibiotic susceptibility patterns of certain cell wall agents (imipenem, cephalothin, and cefepime), as well as in the MALDI-TOF spectra. However, no changes were observed in the SEM images.
Conclusion: The insufficient application of non-thermal plasma in bacterial decontamination may lead to physiological changes that could enrich or select certain subpopulations of S. aureus.
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| first_indexed | 2024-03-12T01:37:11Z |
| format | Article |
| id | doaj.art-6fcbc941f2904432b28c2ca85b3f6bab |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-03-12T01:37:11Z |
| publishDate | 2023-08-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-6fcbc941f2904432b28c2ca85b3f6bab2023-09-11T05:04:03ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472023-08-0115410.18502/ijm.v15i4.13508Non-thermal plasma radiation-induced changes in antibiotic susceptibility and protein profile of Staphylococcus aureusMehrdad Rabbani-Esfahani0Lida Ghaderi1Parisa Shali2Alireza Ghassempour3Seyed Iman Hosseini4Atousa Aliahmadi5Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, IranDepartment of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, IranLaser and Plasma Research Institute, Shahid Beheshti University, Tehran, IranDepartment of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, IranFaculty of Physics, Shahrood University of Technology, Shahrood, IranDepartment of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran Background and Objectives: Plasma radiation is a widely used technique for sterilization or decontamination in various industries, as well as in some healthcare settings such as dentistry. The primary aim of this study was to assess the potential of plasma radiation to create a new population of Staphylococcus aureus cells with distinct characteristics that could lead to novel healthcare challenges. Materials and Methods: A homemade non-thermal plasma apparatus was applied and the effects of plasma treatment on S. aureus ATCC25923 was assessed. Plasma radiation was applied under controlled conditions to ensure that some bacterial cells remained viable. The treatment was repeated 10 times, with each round followed by a recovery phase to collect any surviving bacterial cells. To assess the potential changes in the bacterial population, we examined the antibiotic susceptibility pattern, micro-structural characteristics using scanning electron microscopy (SEM), and total protein profile using the matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) technique. Results: The experimental results revealed slight variations in the antibiotic susceptibility patterns of certain cell wall agents (imipenem, cephalothin, and cefepime), as well as in the MALDI-TOF spectra. However, no changes were observed in the SEM images. Conclusion: The insufficient application of non-thermal plasma in bacterial decontamination may lead to physiological changes that could enrich or select certain subpopulations of S. aureus. https://ijm.tums.ac.ir/index.php/ijm/article/view/3814Plasma radiation;Staphylococcus aureus;Modification;Mass spectrometry |
| spellingShingle | Mehrdad Rabbani-Esfahani Lida Ghaderi Parisa Shali Alireza Ghassempour Seyed Iman Hosseini Atousa Aliahmadi Non-thermal plasma radiation-induced changes in antibiotic susceptibility and protein profile of Staphylococcus aureus Iranian Journal of Microbiology Plasma radiation; Staphylococcus aureus; Modification; Mass spectrometry |
| title | Non-thermal plasma radiation-induced changes in antibiotic susceptibility and protein profile of Staphylococcus aureus |
| title_full | Non-thermal plasma radiation-induced changes in antibiotic susceptibility and protein profile of Staphylococcus aureus |
| title_fullStr | Non-thermal plasma radiation-induced changes in antibiotic susceptibility and protein profile of Staphylococcus aureus |
| title_full_unstemmed | Non-thermal plasma radiation-induced changes in antibiotic susceptibility and protein profile of Staphylococcus aureus |
| title_short | Non-thermal plasma radiation-induced changes in antibiotic susceptibility and protein profile of Staphylococcus aureus |
| title_sort | non thermal plasma radiation induced changes in antibiotic susceptibility and protein profile of staphylococcus aureus |
| topic | Plasma radiation; Staphylococcus aureus; Modification; Mass spectrometry |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/3814 |
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