Hypothermal Effects on Energy Supply for Ionocytes in Gills of Freshwater- and Seawater-Acclimated Milkfish, Chanos chanos
The milkfish, Chanos chanos, is an important aquaculture species that can be cultured in freshwater (FW) and seawater (SW) ponds because of its high euryhalinity. In winter, cold snap leading to high mortality of this tropical species is a critical issue for the aquaculture industry in Taiwan. Under...
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| Format: | Article |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.880103/full |
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| author | Chia-Hao Chang Chia-Hao Chang Chia-Jui Liu Chia-Jui Liu Wei-Jie Lu Li-Yang Wu Kuang-Jie Lai Yu-Ting Lin Yu-Ting Lin Tsung-Han Lee Tsung-Han Lee |
| author_facet | Chia-Hao Chang Chia-Hao Chang Chia-Jui Liu Chia-Jui Liu Wei-Jie Lu Li-Yang Wu Kuang-Jie Lai Yu-Ting Lin Yu-Ting Lin Tsung-Han Lee Tsung-Han Lee |
| author_sort | Chia-Hao Chang |
| collection | DOAJ |
| description | The milkfish, Chanos chanos, is an important aquaculture species that can be cultured in freshwater (FW) and seawater (SW) ponds because of its high euryhalinity. In winter, cold snap leading to high mortality of this tropical species is a critical issue for the aquaculture industry in Taiwan. Under hypothermal stress, however, changes in energy supply for osmoregulation of this euryhaline species is intriguing. In this study, we used an antibody against glycogen phosphorylase (GP) to identify glycogen-rich (GR) cells distributed adjacent to the mitochondria-rich (MR) ionocytes in milkfish gills. Glucose transporter 1 (GLUT1), which plays a major role in energy supply, was also identified and localized in GR cells. Moreover, the expression of indicators of aerobic metabolism and energy production, citrate synthase (CS) and cytochrome c oxidase (COX), were analyzed in gills of FW- and SW-acclimated milkfish to reveal different strategies of energy utilization under hypothermal stress. When exposed to a low-temperature environment, SW individuals used branchial glycogen and lactate to match the energy demands of aerobic metabolism in ionocytes, and elevated aerobic capacity to support and maintain normal functions in gills. However, branchial glycogen mainly localized in the GR cells of FW milkfish was not utilized under hypothermal stress. Meanwhile, a similar level of branchial COX activity and COXIV protein abundance at low temperatures between FW and SW milkfish indicated similar energy requirements in gills. This suggested that another source, but not branchial glycogen, maintained the energy demand in FW milkfish. The present study illustrated differential energy supply strategies in gills between SW- and FW-acclimated milkfish for osmoregulation under low temperatures. |
| first_indexed | 2024-12-10T13:32:27Z |
| format | Article |
| id | doaj.art-6fccd1916f9b451eac26bab79176aafb |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-10T13:32:27Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-6fccd1916f9b451eac26bab79176aafb2022-12-22T01:46:56ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-04-01910.3389/fmars.2022.880103880103Hypothermal Effects on Energy Supply for Ionocytes in Gills of Freshwater- and Seawater-Acclimated Milkfish, Chanos chanosChia-Hao Chang0Chia-Hao Chang1Chia-Jui Liu2Chia-Jui Liu3Wei-Jie Lu4Li-Yang Wu5Kuang-Jie Lai6Yu-Ting Lin7Yu-Ting Lin8Tsung-Han Lee9Tsung-Han Lee10Department of Life Sciences, National Chung Hsing University, Taichung, TaiwanThe integrative Evolutionary Galliform Genomics (iEGG) and Animal Biotechnology Center, National Chung Hsing University, Taichung, TaiwanDepartment of Life Sciences, National Chung Hsing University, Taichung, TaiwanThe integrative Evolutionary Galliform Genomics (iEGG) and Animal Biotechnology Center, National Chung Hsing University, Taichung, TaiwanDepartment of Life Sciences, National Chung Hsing University, Taichung, TaiwanDepartment of Life Sciences, National Chung Hsing University, Taichung, TaiwanDepartment of Life Sciences, National Chung Hsing University, Taichung, TaiwanDepartment of Life Sciences, National Chung Hsing University, Taichung, TaiwanThe integrative Evolutionary Galliform Genomics (iEGG) and Animal Biotechnology Center, National Chung Hsing University, Taichung, TaiwanDepartment of Life Sciences, National Chung Hsing University, Taichung, TaiwanThe integrative Evolutionary Galliform Genomics (iEGG) and Animal Biotechnology Center, National Chung Hsing University, Taichung, TaiwanThe milkfish, Chanos chanos, is an important aquaculture species that can be cultured in freshwater (FW) and seawater (SW) ponds because of its high euryhalinity. In winter, cold snap leading to high mortality of this tropical species is a critical issue for the aquaculture industry in Taiwan. Under hypothermal stress, however, changes in energy supply for osmoregulation of this euryhaline species is intriguing. In this study, we used an antibody against glycogen phosphorylase (GP) to identify glycogen-rich (GR) cells distributed adjacent to the mitochondria-rich (MR) ionocytes in milkfish gills. Glucose transporter 1 (GLUT1), which plays a major role in energy supply, was also identified and localized in GR cells. Moreover, the expression of indicators of aerobic metabolism and energy production, citrate synthase (CS) and cytochrome c oxidase (COX), were analyzed in gills of FW- and SW-acclimated milkfish to reveal different strategies of energy utilization under hypothermal stress. When exposed to a low-temperature environment, SW individuals used branchial glycogen and lactate to match the energy demands of aerobic metabolism in ionocytes, and elevated aerobic capacity to support and maintain normal functions in gills. However, branchial glycogen mainly localized in the GR cells of FW milkfish was not utilized under hypothermal stress. Meanwhile, a similar level of branchial COX activity and COXIV protein abundance at low temperatures between FW and SW milkfish indicated similar energy requirements in gills. This suggested that another source, but not branchial glycogen, maintained the energy demand in FW milkfish. The present study illustrated differential energy supply strategies in gills between SW- and FW-acclimated milkfish for osmoregulation under low temperatures.https://www.frontiersin.org/articles/10.3389/fmars.2022.880103/fullglycogen-rich cellsaerobic metabolismlactateGLUT1milkfishgills |
| spellingShingle | Chia-Hao Chang Chia-Hao Chang Chia-Jui Liu Chia-Jui Liu Wei-Jie Lu Li-Yang Wu Kuang-Jie Lai Yu-Ting Lin Yu-Ting Lin Tsung-Han Lee Tsung-Han Lee Hypothermal Effects on Energy Supply for Ionocytes in Gills of Freshwater- and Seawater-Acclimated Milkfish, Chanos chanos Frontiers in Marine Science glycogen-rich cells aerobic metabolism lactate GLUT1 milkfish gills |
| title | Hypothermal Effects on Energy Supply for Ionocytes in Gills of Freshwater- and Seawater-Acclimated Milkfish, Chanos chanos |
| title_full | Hypothermal Effects on Energy Supply for Ionocytes in Gills of Freshwater- and Seawater-Acclimated Milkfish, Chanos chanos |
| title_fullStr | Hypothermal Effects on Energy Supply for Ionocytes in Gills of Freshwater- and Seawater-Acclimated Milkfish, Chanos chanos |
| title_full_unstemmed | Hypothermal Effects on Energy Supply for Ionocytes in Gills of Freshwater- and Seawater-Acclimated Milkfish, Chanos chanos |
| title_short | Hypothermal Effects on Energy Supply for Ionocytes in Gills of Freshwater- and Seawater-Acclimated Milkfish, Chanos chanos |
| title_sort | hypothermal effects on energy supply for ionocytes in gills of freshwater and seawater acclimated milkfish chanos chanos |
| topic | glycogen-rich cells aerobic metabolism lactate GLUT1 milkfish gills |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.880103/full |
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