Interleukin-1 beta, interferon-gamma, and tumor necrosis factor-alpha gene expression in peripheral blood mononuclear cells of patients with coronary artery disease
<div><p><strong>BACKGROUND:</strong> Several inflammatory mediators have been proposed to contribute to the pathogenesis of atherosclerosis. The aim of this study was to evaluate the quantitative expression of pro-inflammatory cytokines in un-stimulated peripheral blood monon...
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| Format: | Article |
| Language: | English |
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Vesnu Publications
2015-09-01
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| Series: | ARYA Atherosclerosis |
| Subjects: | |
| Online Access: | http://arya.mui.ac.ir/index.php/arya/article/view/898 |
| _version_ | 1819208576451739648 |
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| author | Samaneh Enayati Soroush Seifirad Parvin Amiri Milad Abolhalaj Mahsa Mohammad-Amoli |
| author_facet | Samaneh Enayati Soroush Seifirad Parvin Amiri Milad Abolhalaj Mahsa Mohammad-Amoli |
| author_sort | Samaneh Enayati |
| collection | DOAJ |
| description | <div><p><strong>BACKGROUND:</strong> Several inflammatory mediators have been proposed to contribute to the pathogenesis of atherosclerosis. The aim of this study was to evaluate the quantitative expression of pro-inflammatory cytokines in un-stimulated peripheral blood mononuclear cell of patients with coronary artery disease (CAD).</p> <p><strong>METHODS:</strong> Interleukin-1 beta (IL-1β), tumor necrosis factor-alpha, and interferon-gamma <br /> (IFN-γ) gene expression were evaluated in angiography confirmed patients with and without CAD in a case-control study using quantitative real-time polymerase chain reaction.</p> <p><strong>RESULTS:</strong><strong> </strong>A significant increase (P = 0.030) in IL-1β gene expression was found in patients with CAD [median interquartile range (IQR) = 4.890 (6.084)] compared to patients without CAD [median (IQR) = 1.792 (3.172)]. Despite the increase in IFN-γ gene expression in patients with CAD [median (IQR) = 1.298 (3.896)] versus patients without CAD [median (IQR) = 0.841 (2.79)], there was not statistically significant difference (P = 0.990).</p></div> <strong>CONCLUSION:</strong> Our results provide evidence for possible association between IL-1β and development of atherosclerosis as a crucial cytokine that induce a network of signaling pathways. This finding if proved in future would suggest IL-1β as a potent therapeutic target in CAD.<p> </p> |
| first_indexed | 2024-12-23T05:41:35Z |
| format | Article |
| id | doaj.art-6fcdc7fe0a5645b5b23c4c97e191065a |
| institution | Directory Open Access Journal |
| issn | 1735-3955 2251-6638 |
| language | English |
| last_indexed | 2024-12-23T05:41:35Z |
| publishDate | 2015-09-01 |
| publisher | Vesnu Publications |
| record_format | Article |
| series | ARYA Atherosclerosis |
| spelling | doaj.art-6fcdc7fe0a5645b5b23c4c97e191065a2022-12-21T17:58:11ZengVesnu PublicationsARYA Atherosclerosis1735-39552251-66382015-09-01115267274546Interleukin-1 beta, interferon-gamma, and tumor necrosis factor-alpha gene expression in peripheral blood mononuclear cells of patients with coronary artery diseaseSamaneh Enayati0Soroush Seifirad1Parvin Amiri2Milad Abolhalaj3Mahsa Mohammad-Amoli4Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, IranEndocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute AND Department of Cardiology, Children Medical Center, Tehran University of Medical Sciences, Tehran, IranEndocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, IranEndocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, IranEndocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran<div><p><strong>BACKGROUND:</strong> Several inflammatory mediators have been proposed to contribute to the pathogenesis of atherosclerosis. The aim of this study was to evaluate the quantitative expression of pro-inflammatory cytokines in un-stimulated peripheral blood mononuclear cell of patients with coronary artery disease (CAD).</p> <p><strong>METHODS:</strong> Interleukin-1 beta (IL-1β), tumor necrosis factor-alpha, and interferon-gamma <br /> (IFN-γ) gene expression were evaluated in angiography confirmed patients with and without CAD in a case-control study using quantitative real-time polymerase chain reaction.</p> <p><strong>RESULTS:</strong><strong> </strong>A significant increase (P = 0.030) in IL-1β gene expression was found in patients with CAD [median interquartile range (IQR) = 4.890 (6.084)] compared to patients without CAD [median (IQR) = 1.792 (3.172)]. Despite the increase in IFN-γ gene expression in patients with CAD [median (IQR) = 1.298 (3.896)] versus patients without CAD [median (IQR) = 0.841 (2.79)], there was not statistically significant difference (P = 0.990).</p></div> <strong>CONCLUSION:</strong> Our results provide evidence for possible association between IL-1β and development of atherosclerosis as a crucial cytokine that induce a network of signaling pathways. This finding if proved in future would suggest IL-1β as a potent therapeutic target in CAD.<p> </p>http://arya.mui.ac.ir/index.php/arya/article/view/898Coronary Artery Disease, Interleukin-1 Beta, Tumor Necrosis Factor-alpha, Interferon-gamma, Gene Expression |
| spellingShingle | Samaneh Enayati Soroush Seifirad Parvin Amiri Milad Abolhalaj Mahsa Mohammad-Amoli Interleukin-1 beta, interferon-gamma, and tumor necrosis factor-alpha gene expression in peripheral blood mononuclear cells of patients with coronary artery disease ARYA Atherosclerosis Coronary Artery Disease, Interleukin-1 Beta, Tumor Necrosis Factor-alpha, Interferon-gamma, Gene Expression |
| title | Interleukin-1 beta, interferon-gamma, and tumor necrosis factor-alpha gene expression in peripheral blood mononuclear cells of patients with coronary artery disease |
| title_full | Interleukin-1 beta, interferon-gamma, and tumor necrosis factor-alpha gene expression in peripheral blood mononuclear cells of patients with coronary artery disease |
| title_fullStr | Interleukin-1 beta, interferon-gamma, and tumor necrosis factor-alpha gene expression in peripheral blood mononuclear cells of patients with coronary artery disease |
| title_full_unstemmed | Interleukin-1 beta, interferon-gamma, and tumor necrosis factor-alpha gene expression in peripheral blood mononuclear cells of patients with coronary artery disease |
| title_short | Interleukin-1 beta, interferon-gamma, and tumor necrosis factor-alpha gene expression in peripheral blood mononuclear cells of patients with coronary artery disease |
| title_sort | interleukin 1 beta interferon gamma and tumor necrosis factor alpha gene expression in peripheral blood mononuclear cells of patients with coronary artery disease |
| topic | Coronary Artery Disease, Interleukin-1 Beta, Tumor Necrosis Factor-alpha, Interferon-gamma, Gene Expression |
| url | http://arya.mui.ac.ir/index.php/arya/article/view/898 |
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