MOLECULAR DIVERSITY OF COMMON WHEAT INTROGRESSION LINES (T. AESTIVUM / T. TIMOPHEEVII)
Genetic diversity of T. aestivum/T. timopheevii introgression lines was studied with regard to microsatellite loci and resistance to fungal diseases. Genotyping of hybrid lines and parental common wheat cultivars for 143 SSR markers revealed 521 and 440 alleles, respectively, or 3,24/2,73 alleles pe...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2015-01-01
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| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/295 |
| _version_ | 1797214150350340096 |
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| author | I. N. Leonova O. A. Orlovskaya M. S. Röder M. A. Nesterov E. B. Budashkina |
| author_facet | I. N. Leonova O. A. Orlovskaya M. S. Röder M. A. Nesterov E. B. Budashkina |
| author_sort | I. N. Leonova |
| collection | DOAJ |
| description | Genetic diversity of T. aestivum/T. timopheevii introgression lines was studied with regard to microsatellite loci and resistance to fungal diseases. Genotyping of hybrid lines and parental common wheat cultivars for 143 SSR markers revealed 521 and 440 alleles, respectively, or 3,24/2,73 alleles per microsatellite locus on the average. Comparison of genetic diversity indices of individual chromosomes revealed the lowest diversity for SSR loci on chromosomes 4D and 5D and the highest (0,62–0,67), on 5B and 6A. Evaluation of SSR polymorphisms and indices H in the three genomes of introgression lines indicated that the chromosomes of genome B had higher rates than A or D (B > A > D) which was probably a result of alien introgression into these chromosomes. Comparison of the results of molecular and phytopathological tests allows us to conclude that despite the severe selection for resistance to leaf rust in early generations and a large number of generations of selfing, the genetic diversity of introgression lines on microsatellite loci is preserved, which is indicative of the stability of alien genetic material in the common wheat genome. |
| first_indexed | 2024-03-07T16:08:07Z |
| format | Article |
| id | doaj.art-6fce6f7162b142af9c62adb3770eda0a |
| institution | Directory Open Access Journal |
| issn | 2500-3259 |
| language | English |
| last_indexed | 2024-04-24T11:09:36Z |
| publishDate | 2015-01-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj.art-6fce6f7162b142af9c62adb3770eda0a2024-04-11T15:30:54ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-01-01184/1681690279MOLECULAR DIVERSITY OF COMMON WHEAT INTROGRESSION LINES (T. AESTIVUM / T. TIMOPHEEVII)I. N. Leonova0O. A. Orlovskaya1M. S. Röder2M. A. Nesterov3E. B. Budashkina4Institute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Genetics and Cytology, National Academy of Sciences of Belarus, Minsk, BelarusLeibniz Institute of Plant Genetics and Crop Plant Research, GermanyInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaGenetic diversity of T. aestivum/T. timopheevii introgression lines was studied with regard to microsatellite loci and resistance to fungal diseases. Genotyping of hybrid lines and parental common wheat cultivars for 143 SSR markers revealed 521 and 440 alleles, respectively, or 3,24/2,73 alleles per microsatellite locus on the average. Comparison of genetic diversity indices of individual chromosomes revealed the lowest diversity for SSR loci on chromosomes 4D and 5D and the highest (0,62–0,67), on 5B and 6A. Evaluation of SSR polymorphisms and indices H in the three genomes of introgression lines indicated that the chromosomes of genome B had higher rates than A or D (B > A > D) which was probably a result of alien introgression into these chromosomes. Comparison of the results of molecular and phytopathological tests allows us to conclude that despite the severe selection for resistance to leaf rust in early generations and a large number of generations of selfing, the genetic diversity of introgression lines on microsatellite loci is preserved, which is indicative of the stability of alien genetic material in the common wheat genome.https://vavilov.elpub.ru/jour/article/view/295genetic diversityfungal diseasesintrogression linescommon wheat t. aestivumssr markerst. timopheevii |
| spellingShingle | I. N. Leonova O. A. Orlovskaya M. S. Röder M. A. Nesterov E. B. Budashkina MOLECULAR DIVERSITY OF COMMON WHEAT INTROGRESSION LINES (T. AESTIVUM / T. TIMOPHEEVII) Вавиловский журнал генетики и селекции genetic diversity fungal diseases introgression lines common wheat t. aestivum ssr markers t. timopheevii |
| title | MOLECULAR DIVERSITY OF COMMON WHEAT INTROGRESSION LINES (T. AESTIVUM / T. TIMOPHEEVII) |
| title_full | MOLECULAR DIVERSITY OF COMMON WHEAT INTROGRESSION LINES (T. AESTIVUM / T. TIMOPHEEVII) |
| title_fullStr | MOLECULAR DIVERSITY OF COMMON WHEAT INTROGRESSION LINES (T. AESTIVUM / T. TIMOPHEEVII) |
| title_full_unstemmed | MOLECULAR DIVERSITY OF COMMON WHEAT INTROGRESSION LINES (T. AESTIVUM / T. TIMOPHEEVII) |
| title_short | MOLECULAR DIVERSITY OF COMMON WHEAT INTROGRESSION LINES (T. AESTIVUM / T. TIMOPHEEVII) |
| title_sort | molecular diversity of common wheat introgression lines t aestivum t timopheevii |
| topic | genetic diversity fungal diseases introgression lines common wheat t. aestivum ssr markers t. timopheevii |
| url | https://vavilov.elpub.ru/jour/article/view/295 |
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