Development of embryoids by microspore and anther cultures of red beet (Beta vulgaris L. subsp. vulgaris)
So far there is no information about receiving red beet androgenic embryos by androgenesis. Several factors were tested which affected this process: starch accumulation in microspores, correlation between bud length and microsporogenesis course, induction and regeneration medium composition. Ploidy...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Bulgarian |
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University of Zagreb, Faculty of Agriculture
2017-03-01
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| Series: | Journal of Central European Agriculture |
| Subjects: | |
| Online Access: | http://jcea.agr.hr/articles/773930_Development_of_embryoids_by_microspore_and_anther_cultures_of_red_beet_(Beta_vulgaris_L_subsp_vulgaris)_en.pdf |
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| author | Krystyna GÓRECKA Dorota KRZYŻANOWSKA Urszula KOWALSKA Waldemar KISZCZAK Małgorzata PODWYSZYŃSKA |
| author_facet | Krystyna GÓRECKA Dorota KRZYŻANOWSKA Urszula KOWALSKA Waldemar KISZCZAK Małgorzata PODWYSZYŃSKA |
| author_sort | Krystyna GÓRECKA |
| collection | DOAJ |
| description | So far there is no information about receiving red beet androgenic embryos by androgenesis. Several factors were tested which affected this process: starch accumulation in microspores, correlation between bud length and microsporogenesis course, induction and regeneration medium composition. Ploidy level of obtained regenerants were evaluated. Treating anthers with α-amylase or watering donor plants with gibberellin increased number of obtained androgenic embryos. The highest percentage (80%) of microspores at uninuclear stage appeared in buds with 1.3-1.5 mm. The B5 medium with 100 g·L-1 sucrose and 0.1 mg·L-1 2,4-D (2, 4-dichlorophenoxyacetic acid) proved to be better for inducing androgenesis than MS medium supplemented with 0.2 mg·L-1 BAP (6-benzylaminopurine) and 0.5 mg·L-1 IAA (indole-3-acetic acid). First androgenic embryos were placed on B5 medium without plant growth regulators and then on MS medium containing 0.2 mg·L-1 BAP and 1 mg·L-1 NAA (α-naphthaleneacetic acid). Androgenic embryos died on B5 regeneration medium without plant growth regulators. On MS medium first shoots and callus with and without roots were obtained. Rosettes withered during following passages whereas callus tissue developed further. The quantity of DNA in this tissue equivalent to 4X chromosomes. |
| first_indexed | 2024-12-12T17:01:49Z |
| format | Article |
| id | doaj.art-6fd06567d3c348b8af132f271b8371d1 |
| institution | Directory Open Access Journal |
| issn | 1332-9049 |
| language | Bulgarian |
| last_indexed | 2024-12-12T17:01:49Z |
| publishDate | 2017-03-01 |
| publisher | University of Zagreb, Faculty of Agriculture |
| record_format | Article |
| series | Journal of Central European Agriculture |
| spelling | doaj.art-6fd06567d3c348b8af132f271b8371d12022-12-22T00:18:07ZbulUniversity of Zagreb, Faculty of AgricultureJournal of Central European Agriculture1332-90492017-03-0118118519510.5513/JCEA01/18.1.1877Development of embryoids by microspore and anther cultures of red beet (Beta vulgaris L. subsp. vulgaris)Krystyna GÓRECKADorota KRZYŻANOWSKAUrszula KOWALSKAWaldemar KISZCZAKMałgorzata PODWYSZYŃSKASo far there is no information about receiving red beet androgenic embryos by androgenesis. Several factors were tested which affected this process: starch accumulation in microspores, correlation between bud length and microsporogenesis course, induction and regeneration medium composition. Ploidy level of obtained regenerants were evaluated. Treating anthers with α-amylase or watering donor plants with gibberellin increased number of obtained androgenic embryos. The highest percentage (80%) of microspores at uninuclear stage appeared in buds with 1.3-1.5 mm. The B5 medium with 100 g·L-1 sucrose and 0.1 mg·L-1 2,4-D (2, 4-dichlorophenoxyacetic acid) proved to be better for inducing androgenesis than MS medium supplemented with 0.2 mg·L-1 BAP (6-benzylaminopurine) and 0.5 mg·L-1 IAA (indole-3-acetic acid). First androgenic embryos were placed on B5 medium without plant growth regulators and then on MS medium containing 0.2 mg·L-1 BAP and 1 mg·L-1 NAA (α-naphthaleneacetic acid). Androgenic embryos died on B5 regeneration medium without plant growth regulators. On MS medium first shoots and callus with and without roots were obtained. Rosettes withered during following passages whereas callus tissue developed further. The quantity of DNA in this tissue equivalent to 4X chromosomes.http://jcea.agr.hr/articles/773930_Development_of_embryoids_by_microspore_and_anther_cultures_of_red_beet_(Beta_vulgaris_L_subsp_vulgaris)_en.pdfandrogenesisanther cultureisolated microsporemicrosporogenesisploidyred beet |
| spellingShingle | Krystyna GÓRECKA Dorota KRZYŻANOWSKA Urszula KOWALSKA Waldemar KISZCZAK Małgorzata PODWYSZYŃSKA Development of embryoids by microspore and anther cultures of red beet (Beta vulgaris L. subsp. vulgaris) Journal of Central European Agriculture androgenesis anther culture isolated microspore microsporogenesis ploidy red beet |
| title | Development of embryoids by microspore and anther cultures of red beet (Beta vulgaris L. subsp. vulgaris) |
| title_full | Development of embryoids by microspore and anther cultures of red beet (Beta vulgaris L. subsp. vulgaris) |
| title_fullStr | Development of embryoids by microspore and anther cultures of red beet (Beta vulgaris L. subsp. vulgaris) |
| title_full_unstemmed | Development of embryoids by microspore and anther cultures of red beet (Beta vulgaris L. subsp. vulgaris) |
| title_short | Development of embryoids by microspore and anther cultures of red beet (Beta vulgaris L. subsp. vulgaris) |
| title_sort | development of embryoids by microspore and anther cultures of red beet beta vulgaris l subsp vulgaris |
| topic | androgenesis anther culture isolated microspore microsporogenesis ploidy red beet |
| url | http://jcea.agr.hr/articles/773930_Development_of_embryoids_by_microspore_and_anther_cultures_of_red_beet_(Beta_vulgaris_L_subsp_vulgaris)_en.pdf |
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