A simple and cost-effective diagnostic of Macrophomina phaseolina on watermelon by direct PCR
Macrophomina phaseolina (Tassi) Goid is the causal agent of charcoal rot and vine decline in cucurbits such as watermelon. Molecular methods have been used for rapid identification. However, a large number of steps used reduces its applicability. This study aimed to detect M. phaseolina in watermel...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Eduem (Editora da Universidade Estadual de Maringá)
2022-09-01
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| Series: | Acta Scientiarum: Agronomy |
| Subjects: | |
| Online Access: | https://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/56141 |
| _version_ | 1811272453391908864 |
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| author | Suzana Marjorie Freire e Silva Gilsivan Sales Medeiros de Aquino Talison Eugenio da Costa Anna Luisa de Carvalho Brito Andréia Mitsa Paiva Negreiros Rui Sales Júnior Tatsuya Nagata Ioná Araujo Santos Holanda |
| author_facet | Suzana Marjorie Freire e Silva Gilsivan Sales Medeiros de Aquino Talison Eugenio da Costa Anna Luisa de Carvalho Brito Andréia Mitsa Paiva Negreiros Rui Sales Júnior Tatsuya Nagata Ioná Araujo Santos Holanda |
| author_sort | Suzana Marjorie Freire e Silva |
| collection | DOAJ |
| description |
Macrophomina phaseolina (Tassi) Goid is the causal agent of charcoal rot and vine decline in cucurbits such as watermelon. Molecular methods have been used for rapid identification. However, a large number of steps used reduces its applicability. This study aimed to detect M. phaseolina in watermelon from producing areas in Northeastern Brazil by direct PCR. Plant tissue samples were collected from seven producing areas and the DNA was extracted using the CTAB method. Amplifications were performed by direct PCR using the MpKFI/MpKRI primers, then the PCR products were subjected to agarose gel electrophoresis and sequenced. Amplicons of 350 bp were observed in stem tissue samples from three areas. The identity of the samples was confirmed by sequencing. This study represents the first molecular diagnosis of M. phaseolina associated with watermelon in Northeastern Brazil. The methodology presented here can be applied for a reliable and simple diagnosis of the pathogen in other crops.
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| first_indexed | 2024-04-12T22:40:31Z |
| format | Article |
| id | doaj.art-6fd0f56da434463a844bb26f625d4d9c |
| institution | Directory Open Access Journal |
| issn | 1679-9275 1807-8621 |
| language | English |
| last_indexed | 2024-04-12T22:40:31Z |
| publishDate | 2022-09-01 |
| publisher | Eduem (Editora da Universidade Estadual de Maringá) |
| record_format | Article |
| series | Acta Scientiarum: Agronomy |
| spelling | doaj.art-6fd0f56da434463a844bb26f625d4d9c2022-12-22T03:13:44ZengEduem (Editora da Universidade Estadual de Maringá)Acta Scientiarum: Agronomy1679-92751807-86212022-09-0145110.4025/actasciagron.v45i1.56141A simple and cost-effective diagnostic of Macrophomina phaseolina on watermelon by direct PCRSuzana Marjorie Freire e Silva0Gilsivan Sales Medeiros de Aquino1Talison Eugenio da Costa2Anna Luisa de Carvalho Brito3Andréia Mitsa Paiva Negreiros4Rui Sales Júnior5Tatsuya Nagata6Ioná Araujo Santos Holanda7Universidade Federal Rural do Semi-ÁridoUniversidade Federal Rural do Semi-ÁridoUniversidade Federal Rural do Semi-ÁridoUniversidade Federal Rural do Semi-ÁridoUniversidade Federal Rural do Semi-ÁridoUniversidade Federal Rural do Semi-ÁridoUniversidade de BrasiliaUniversidade Federal Rural do Semi-Árido Macrophomina phaseolina (Tassi) Goid is the causal agent of charcoal rot and vine decline in cucurbits such as watermelon. Molecular methods have been used for rapid identification. However, a large number of steps used reduces its applicability. This study aimed to detect M. phaseolina in watermelon from producing areas in Northeastern Brazil by direct PCR. Plant tissue samples were collected from seven producing areas and the DNA was extracted using the CTAB method. Amplifications were performed by direct PCR using the MpKFI/MpKRI primers, then the PCR products were subjected to agarose gel electrophoresis and sequenced. Amplicons of 350 bp were observed in stem tissue samples from three areas. The identity of the samples was confirmed by sequencing. This study represents the first molecular diagnosis of M. phaseolina associated with watermelon in Northeastern Brazil. The methodology presented here can be applied for a reliable and simple diagnosis of the pathogen in other crops. https://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/56141charcoal rot; Citrullus lanatus; direct PCR; ITS primer. |
| spellingShingle | Suzana Marjorie Freire e Silva Gilsivan Sales Medeiros de Aquino Talison Eugenio da Costa Anna Luisa de Carvalho Brito Andréia Mitsa Paiva Negreiros Rui Sales Júnior Tatsuya Nagata Ioná Araujo Santos Holanda A simple and cost-effective diagnostic of Macrophomina phaseolina on watermelon by direct PCR Acta Scientiarum: Agronomy charcoal rot; Citrullus lanatus; direct PCR; ITS primer. |
| title | A simple and cost-effective diagnostic of Macrophomina phaseolina on watermelon by direct PCR |
| title_full | A simple and cost-effective diagnostic of Macrophomina phaseolina on watermelon by direct PCR |
| title_fullStr | A simple and cost-effective diagnostic of Macrophomina phaseolina on watermelon by direct PCR |
| title_full_unstemmed | A simple and cost-effective diagnostic of Macrophomina phaseolina on watermelon by direct PCR |
| title_short | A simple and cost-effective diagnostic of Macrophomina phaseolina on watermelon by direct PCR |
| title_sort | simple and cost effective diagnostic of macrophomina phaseolina on watermelon by direct pcr |
| topic | charcoal rot; Citrullus lanatus; direct PCR; ITS primer. |
| url | https://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/56141 |
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