Micellar liquid chromatographic determination of sertaconazole and terconazole in bulk, pharmaceutical dosage forms and spiked human plasma
A micellar liquid chromatographic method was developed for the determination of sertaconazole and terconazole in bulk, dosage forms and human plasma using intersil cyano column and mobile phase consisting of 0.1 M sodium dodecyl sulphate, 20% 1-propanol, and 0.3% triethylamine in 0.02 M ortho-phosph...
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| Format: | Article |
| Language: | English |
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Faculty of Pharmacy, Cairo University
2014-06-01
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| Series: | Bulletin of Faculty of Pharmacy Cairo University |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S111009311400012X |
| _version_ | 1797964827259305984 |
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| author | Mohamed Rizk Safaa S. Toubar Maha M.A. El-Alamin Marwa M.M. Azab |
| author_facet | Mohamed Rizk Safaa S. Toubar Maha M.A. El-Alamin Marwa M.M. Azab |
| author_sort | Mohamed Rizk |
| collection | DOAJ |
| description | A micellar liquid chromatographic method was developed for the determination of sertaconazole and terconazole in bulk, dosage forms and human plasma using intersil cyano column and mobile phase consisting of 0.1 M sodium dodecyl sulphate, 20% 1-propanol, and 0.3% triethylamine in 0.02 M ortho-phosphoric acid (pH 4) at 225 nm. Different chromatographic parameters were studied, e.g. types of columns, pH of mobile phase, concentration of sodium dodecyl sulphate, 1-propanol, triethylamine, etc. The method was validated over the concentration ranges 8–40 and 16–80 μg/ml, for sertaconazole and terconazole, respectively. The method was sensitive with limits of detection of 1.24 and 1.67 μg/ml for sertaconazole and terconazole in bulk, respectively. Inter and intra-day results showed % RSD < 0.9% and 1.55% for sertaconazole and terconazole, respectively. The result obtained by the proposed method was compared with that obtained by the reference HPLC technique. Furthermore, the proposed method was successfully applied as a stability-indicating method for the determination of drugs under different stressed conditions. The method showed good selectivity, repeatability, linearity and sensitivity according to the evaluation of the validation parameters. |
| first_indexed | 2024-04-11T01:49:54Z |
| format | Article |
| id | doaj.art-6fd25775c1504ca3ace0a9ceda9aef09 |
| institution | Directory Open Access Journal |
| issn | 1110-0931 |
| language | English |
| last_indexed | 2024-04-11T01:49:54Z |
| publishDate | 2014-06-01 |
| publisher | Faculty of Pharmacy, Cairo University |
| record_format | Article |
| series | Bulletin of Faculty of Pharmacy Cairo University |
| spelling | doaj.art-6fd25775c1504ca3ace0a9ceda9aef092023-01-03T06:43:20ZengFaculty of Pharmacy, Cairo UniversityBulletin of Faculty of Pharmacy Cairo University1110-09312014-06-0152115516410.1016/j.bfopcu.2014.03.002Micellar liquid chromatographic determination of sertaconazole and terconazole in bulk, pharmaceutical dosage forms and spiked human plasmaMohamed RizkSafaa S. ToubarMaha M.A. El-AlaminMarwa M.M. AzabA micellar liquid chromatographic method was developed for the determination of sertaconazole and terconazole in bulk, dosage forms and human plasma using intersil cyano column and mobile phase consisting of 0.1 M sodium dodecyl sulphate, 20% 1-propanol, and 0.3% triethylamine in 0.02 M ortho-phosphoric acid (pH 4) at 225 nm. Different chromatographic parameters were studied, e.g. types of columns, pH of mobile phase, concentration of sodium dodecyl sulphate, 1-propanol, triethylamine, etc. The method was validated over the concentration ranges 8–40 and 16–80 μg/ml, for sertaconazole and terconazole, respectively. The method was sensitive with limits of detection of 1.24 and 1.67 μg/ml for sertaconazole and terconazole in bulk, respectively. Inter and intra-day results showed % RSD < 0.9% and 1.55% for sertaconazole and terconazole, respectively. The result obtained by the proposed method was compared with that obtained by the reference HPLC technique. Furthermore, the proposed method was successfully applied as a stability-indicating method for the determination of drugs under different stressed conditions. The method showed good selectivity, repeatability, linearity and sensitivity according to the evaluation of the validation parameters.http://www.sciencedirect.com/science/article/pii/S111009311400012XAntifungal drugsMicellar liquid chromatographySertaconazoleTerconazoleMethod validationStability |
| spellingShingle | Mohamed Rizk Safaa S. Toubar Maha M.A. El-Alamin Marwa M.M. Azab Micellar liquid chromatographic determination of sertaconazole and terconazole in bulk, pharmaceutical dosage forms and spiked human plasma Bulletin of Faculty of Pharmacy Cairo University Antifungal drugs Micellar liquid chromatography Sertaconazole Terconazole Method validation Stability |
| title | Micellar liquid chromatographic determination of sertaconazole and terconazole in bulk, pharmaceutical dosage forms and spiked human plasma |
| title_full | Micellar liquid chromatographic determination of sertaconazole and terconazole in bulk, pharmaceutical dosage forms and spiked human plasma |
| title_fullStr | Micellar liquid chromatographic determination of sertaconazole and terconazole in bulk, pharmaceutical dosage forms and spiked human plasma |
| title_full_unstemmed | Micellar liquid chromatographic determination of sertaconazole and terconazole in bulk, pharmaceutical dosage forms and spiked human plasma |
| title_short | Micellar liquid chromatographic determination of sertaconazole and terconazole in bulk, pharmaceutical dosage forms and spiked human plasma |
| title_sort | micellar liquid chromatographic determination of sertaconazole and terconazole in bulk pharmaceutical dosage forms and spiked human plasma |
| topic | Antifungal drugs Micellar liquid chromatography Sertaconazole Terconazole Method validation Stability |
| url | http://www.sciencedirect.com/science/article/pii/S111009311400012X |
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