Improved cytometric analysis of untouched lung leukocytes by enzymatic liquefaction of sputum samples

Abstract Background Phenotyping sputum-resident leukocytes and evaluating their functional status are essential analyses for exploring the cellular basis of pathological processes in the lungs, and flow cytometry is widely recognized as the gold-standard technique to address them. However, sputum-re...

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Bibliographic Details
Main Authors: Giulia Santopolo, Antonio Clemente, Estrella Rojo-Molinero, Sara Fernández, María Concepción Álvarez, Antonio Oliver, Roberto de la Rica
Format: Article
Language:English
Published: BMC 2022-11-01
Series:Biological Procedures Online
Subjects:
Online Access:https://doi.org/10.1186/s12575-022-00181-z