Neuroprotective Profile of Edible Flowers of Borage (<i>Borago officinalis</i> L.) in Two Different Models: <i>Caenorhabditis elegans</i> and Neuro-2a Cells
The flowers of <i>Borago officinalis</i> L. (Boraginaceae), commonly known as borage, are widely used as a culinary ingredient. The aim of this study was to assess the potential benefits of fresh borage flower extract related to antioxidant, neuroprotective and anti-aging properties. The...
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MDPI AG
2022-06-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/7/1244 |
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| author | Cristina Moliner Guillermo Cásedas Lillian Barros Tiane C. Finimundy Carlota Gómez-Rincón Víctor López |
| author_facet | Cristina Moliner Guillermo Cásedas Lillian Barros Tiane C. Finimundy Carlota Gómez-Rincón Víctor López |
| author_sort | Cristina Moliner |
| collection | DOAJ |
| description | The flowers of <i>Borago officinalis</i> L. (Boraginaceae), commonly known as borage, are widely used as a culinary ingredient. The aim of this study was to assess the potential benefits of fresh borage flower extract related to antioxidant, neuroprotective and anti-aging properties. The extract was obtained by Soxhlet extraction with ethanol as a solvent, and fatty acids were detected by GC-FID. The antioxidant activity was evaluated in vitro through the DPPH, FRAP and ORAC assays. Regarding the fatty acid (FA) composition, the extract showed high amounts of polyunsaturated FA. The Neuro-2a cell line was used to determine the cytoprotective capacity of the extract subjected to oxidative stress (H<sub>2</sub>O<sub>2</sub>). Moreover, the model organism <i>Caenorhabditis elegans</i> was used to assess antioxidant activity, delayed ageing as well as cytoprotection and reduced β-amyloid toxicity. Cells treated with the extract and H<sub>2</sub>O<sub>2</sub> showed a better response to oxidative stress than the control group, particularly in terms of mitochondrial activity (MTT assay), redox state (ROS formation) and the activity of antioxidant enzymes (catalase and superoxide dismutase). <i>B. officinalis</i> flower extract showed promising antioxidant activity in the selected models, without causing toxicity. Hence, the results obtained support the antioxidant properties of borage flowers in different bioassays using living organisms. |
| first_indexed | 2024-03-09T10:23:14Z |
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| id | doaj.art-6fd6ab58922c4501bbbf2f5e7f13deaf |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T10:23:14Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-6fd6ab58922c4501bbbf2f5e7f13deaf2023-12-01T21:49:28ZengMDPI AGAntioxidants2076-39212022-06-01117124410.3390/antiox11071244Neuroprotective Profile of Edible Flowers of Borage (<i>Borago officinalis</i> L.) in Two Different Models: <i>Caenorhabditis elegans</i> and Neuro-2a CellsCristina Moliner0Guillermo Cásedas1Lillian Barros2Tiane C. Finimundy3Carlota Gómez-Rincón4Víctor López5Department of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, 50830 Zaragoza, SpainDepartment of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, 50830 Zaragoza, SpainCentro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, PortugalCentro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, PortugalDepartment of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, 50830 Zaragoza, SpainDepartment of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, 50830 Zaragoza, SpainThe flowers of <i>Borago officinalis</i> L. (Boraginaceae), commonly known as borage, are widely used as a culinary ingredient. The aim of this study was to assess the potential benefits of fresh borage flower extract related to antioxidant, neuroprotective and anti-aging properties. The extract was obtained by Soxhlet extraction with ethanol as a solvent, and fatty acids were detected by GC-FID. The antioxidant activity was evaluated in vitro through the DPPH, FRAP and ORAC assays. Regarding the fatty acid (FA) composition, the extract showed high amounts of polyunsaturated FA. The Neuro-2a cell line was used to determine the cytoprotective capacity of the extract subjected to oxidative stress (H<sub>2</sub>O<sub>2</sub>). Moreover, the model organism <i>Caenorhabditis elegans</i> was used to assess antioxidant activity, delayed ageing as well as cytoprotection and reduced β-amyloid toxicity. Cells treated with the extract and H<sub>2</sub>O<sub>2</sub> showed a better response to oxidative stress than the control group, particularly in terms of mitochondrial activity (MTT assay), redox state (ROS formation) and the activity of antioxidant enzymes (catalase and superoxide dismutase). <i>B. officinalis</i> flower extract showed promising antioxidant activity in the selected models, without causing toxicity. Hence, the results obtained support the antioxidant properties of borage flowers in different bioassays using living organisms.https://www.mdpi.com/2076-3921/11/7/1244<i>Borago officinalis</i>PUFAantioxidantneuroprotection<i>C. elegans</i>Neuro-2a |
| spellingShingle | Cristina Moliner Guillermo Cásedas Lillian Barros Tiane C. Finimundy Carlota Gómez-Rincón Víctor López Neuroprotective Profile of Edible Flowers of Borage (<i>Borago officinalis</i> L.) in Two Different Models: <i>Caenorhabditis elegans</i> and Neuro-2a Cells Antioxidants <i>Borago officinalis</i> PUFA antioxidant neuroprotection <i>C. elegans</i> Neuro-2a |
| title | Neuroprotective Profile of Edible Flowers of Borage (<i>Borago officinalis</i> L.) in Two Different Models: <i>Caenorhabditis elegans</i> and Neuro-2a Cells |
| title_full | Neuroprotective Profile of Edible Flowers of Borage (<i>Borago officinalis</i> L.) in Two Different Models: <i>Caenorhabditis elegans</i> and Neuro-2a Cells |
| title_fullStr | Neuroprotective Profile of Edible Flowers of Borage (<i>Borago officinalis</i> L.) in Two Different Models: <i>Caenorhabditis elegans</i> and Neuro-2a Cells |
| title_full_unstemmed | Neuroprotective Profile of Edible Flowers of Borage (<i>Borago officinalis</i> L.) in Two Different Models: <i>Caenorhabditis elegans</i> and Neuro-2a Cells |
| title_short | Neuroprotective Profile of Edible Flowers of Borage (<i>Borago officinalis</i> L.) in Two Different Models: <i>Caenorhabditis elegans</i> and Neuro-2a Cells |
| title_sort | neuroprotective profile of edible flowers of borage i borago officinalis i l in two different models i caenorhabditis elegans i and neuro 2a cells |
| topic | <i>Borago officinalis</i> PUFA antioxidant neuroprotection <i>C. elegans</i> Neuro-2a |
| url | https://www.mdpi.com/2076-3921/11/7/1244 |
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