Detection of anti-SARS-CoV-2 salivary antibodies in vaccinated adults
Since the introduction of efficient anti-SARS-CoV-2 vaccines, the detection of antibodies becomes useful for immunological monitoring and COVID-19 control. Therefore, this longitudinal study aimed to evaluate the detection of SARS-CoV-2 antibodies in the serum and saliva of COVID-19-vaccinated adult...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1296603/full |
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| author | Vitória Tavares Castro Hélène Chardin Hélène Chardin Juliana Amorim dos Santos Gustavo Barcelos Barra Grazielle Rodrigues Castilho Paula Monteiro Souza Pérola de Oliveira Magalhães Ana Carolina Acevedo Eliete Neves Silva Guerra |
| author_facet | Vitória Tavares Castro Hélène Chardin Hélène Chardin Juliana Amorim dos Santos Gustavo Barcelos Barra Grazielle Rodrigues Castilho Paula Monteiro Souza Pérola de Oliveira Magalhães Ana Carolina Acevedo Eliete Neves Silva Guerra |
| author_sort | Vitória Tavares Castro |
| collection | DOAJ |
| description | Since the introduction of efficient anti-SARS-CoV-2 vaccines, the detection of antibodies becomes useful for immunological monitoring and COVID-19 control. Therefore, this longitudinal study aimed to evaluate the detection of SARS-CoV-2 antibodies in the serum and saliva of COVID-19-vaccinated adults. The study included 13 not vaccinated and 35 vaccinated participants with two doses of CoronaVac (Sinovac/Butantan) vaccine who subsequently received BNT162b2 (Pfizer-BioNTech) vaccine as a booster dose. Vaccinated participants donated saliva and serum in three different time points. Enzyme-linked immunosorbent assay was used for antibody detection. In our results, the serum neutralizing antibodies (NAb) were detected in 34/35 samples after second dose and in 35/35 samples one and five months after the booster dose. In saliva, NAb were detected in 30/35 samples after second dose and in 35/35 of samples one and five months after the booster dose. IgA was detected in 19/34 saliva samples after second dose, in 18/35 one month after the booster and in 30/35 five months after. IgG in saliva was detected in 1/34 samples after second dose, 33/35 samples one month after the booster dose and in 20/35 five months after. A strong correlation was found between IgG and neutralizing activity in saliva, and salivary IgA would be a sign of recent exposure to the virus. In conclusion, saliva can be suitable for monitoring antibodies anti-SARS-CoV-2 after vaccination. Heterologous vaccination contributed to increase anti-SARS-CoV-2 antibodies in the Brazilian health context. Complementary studies with large groups are mandatory to conclude the interest in following mucosal immunity. |
| first_indexed | 2024-03-11T12:15:43Z |
| format | Article |
| id | doaj.art-6fdd8a37ed54462bb9f982d6c0dcfe13 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-11T12:15:43Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-6fdd8a37ed54462bb9f982d6c0dcfe132023-11-07T09:57:36ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-11-011410.3389/fimmu.2023.12966031296603Detection of anti-SARS-CoV-2 salivary antibodies in vaccinated adultsVitória Tavares Castro0Hélène Chardin1Hélène Chardin2Juliana Amorim dos Santos3Gustavo Barcelos Barra4Grazielle Rodrigues Castilho5Paula Monteiro Souza6Pérola de Oliveira Magalhães7Ana Carolina Acevedo8Eliete Neves Silva Guerra9Laboratory of Oral Histopathology, Faculty of Health Sciences, University of Brasilia, Brasília, DF, BrazilDepartment of Analytical, Bioanalytical Sciences and Miniaturization, École Supérieure de Physique et de Chimie Industrielles (ESPCI) de la Ville de Paris, Paris, FranceUnité de Formation et de Recherche d’Odontologie, Université Paris Cité, Paris, FranceLaboratory of Oral Histopathology, Faculty of Health Sciences, University of Brasilia, Brasília, DF, BrazilSabin Medicina Diagnostica Laboratory, Institute Sabin, Brasília, DF, BrazilSabin Medicina Diagnostica Laboratory, Institute Sabin, Brasília, DF, BrazilLaboratory of Natural Products, Faculty of Health Sciences, University of Brasilia, Brasília, DF, BrazilLaboratory of Natural Products, Faculty of Health Sciences, University of Brasilia, Brasília, DF, BrazilLaboratory of Oral Histopathology, Faculty of Health Sciences, University of Brasilia, Brasília, DF, BrazilLaboratory of Oral Histopathology, Faculty of Health Sciences, University of Brasilia, Brasília, DF, BrazilSince the introduction of efficient anti-SARS-CoV-2 vaccines, the detection of antibodies becomes useful for immunological monitoring and COVID-19 control. Therefore, this longitudinal study aimed to evaluate the detection of SARS-CoV-2 antibodies in the serum and saliva of COVID-19-vaccinated adults. The study included 13 not vaccinated and 35 vaccinated participants with two doses of CoronaVac (Sinovac/Butantan) vaccine who subsequently received BNT162b2 (Pfizer-BioNTech) vaccine as a booster dose. Vaccinated participants donated saliva and serum in three different time points. Enzyme-linked immunosorbent assay was used for antibody detection. In our results, the serum neutralizing antibodies (NAb) were detected in 34/35 samples after second dose and in 35/35 samples one and five months after the booster dose. In saliva, NAb were detected in 30/35 samples after second dose and in 35/35 of samples one and five months after the booster dose. IgA was detected in 19/34 saliva samples after second dose, in 18/35 one month after the booster and in 30/35 five months after. IgG in saliva was detected in 1/34 samples after second dose, 33/35 samples one month after the booster dose and in 20/35 five months after. A strong correlation was found between IgG and neutralizing activity in saliva, and salivary IgA would be a sign of recent exposure to the virus. In conclusion, saliva can be suitable for monitoring antibodies anti-SARS-CoV-2 after vaccination. Heterologous vaccination contributed to increase anti-SARS-CoV-2 antibodies in the Brazilian health context. Complementary studies with large groups are mandatory to conclude the interest in following mucosal immunity.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1296603/fullCOVID-19SARS-CoV-2salivaantibodiesneutralizing antibodiesIgG |
| spellingShingle | Vitória Tavares Castro Hélène Chardin Hélène Chardin Juliana Amorim dos Santos Gustavo Barcelos Barra Grazielle Rodrigues Castilho Paula Monteiro Souza Pérola de Oliveira Magalhães Ana Carolina Acevedo Eliete Neves Silva Guerra Detection of anti-SARS-CoV-2 salivary antibodies in vaccinated adults Frontiers in Immunology COVID-19 SARS-CoV-2 saliva antibodies neutralizing antibodies IgG |
| title | Detection of anti-SARS-CoV-2 salivary antibodies in vaccinated adults |
| title_full | Detection of anti-SARS-CoV-2 salivary antibodies in vaccinated adults |
| title_fullStr | Detection of anti-SARS-CoV-2 salivary antibodies in vaccinated adults |
| title_full_unstemmed | Detection of anti-SARS-CoV-2 salivary antibodies in vaccinated adults |
| title_short | Detection of anti-SARS-CoV-2 salivary antibodies in vaccinated adults |
| title_sort | detection of anti sars cov 2 salivary antibodies in vaccinated adults |
| topic | COVID-19 SARS-CoV-2 saliva antibodies neutralizing antibodies IgG |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1296603/full |
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