The Phage Nucleus and Tubulin Spindle Are Conserved among Large Pseudomonas Phages
We recently demonstrated that the large Pseudomonas chlororaphis bacteriophage 201φ2-1 assembles a nucleus-like structure that encloses phage DNA and segregates proteins according to function, with DNA processing proteins inside and metabolic enzymes and ribosomes outside the nucleus. Here, we inves...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-08-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124717310574 |
| _version_ | 1818204094479728640 |
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| author | Vorrapon Chaikeeratisak Katrina Nguyen MacKennon E. Egan Marcella L. Erb Anastasia Vavilina Joe Pogliano |
| author_facet | Vorrapon Chaikeeratisak Katrina Nguyen MacKennon E. Egan Marcella L. Erb Anastasia Vavilina Joe Pogliano |
| author_sort | Vorrapon Chaikeeratisak |
| collection | DOAJ |
| description | We recently demonstrated that the large Pseudomonas chlororaphis bacteriophage 201φ2-1 assembles a nucleus-like structure that encloses phage DNA and segregates proteins according to function, with DNA processing proteins inside and metabolic enzymes and ribosomes outside the nucleus. Here, we investigate the replication pathway of the Pseudomonas aeruginosa bacteriophages φKZ and φPA3. Bacteriophages φKZ and φPA3 encode a proteinaceous shell that assembles a nucleus-like structure that compartmentalizes proteins and DNA during viral infection. We show that the tubulin-like protein PhuZ encoded by each phage assembles a bipolar spindle that displays dynamic instability and positions the nucleus at midcell. Our results suggest that the phage spindle and nucleus play the same functional role in all three phages, 201φ2-1, φKZ, and φPA3, demonstrating that these key structures are conserved among large Pseudomonas phages. |
| first_indexed | 2024-12-12T03:35:46Z |
| format | Article |
| id | doaj.art-6fdde347824a4b149a3f9cc346cc27ee |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-12T03:35:46Z |
| publishDate | 2017-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-6fdde347824a4b149a3f9cc346cc27ee2022-12-22T00:39:49ZengElsevierCell Reports2211-12472017-08-012071563157110.1016/j.celrep.2017.07.064The Phage Nucleus and Tubulin Spindle Are Conserved among Large Pseudomonas PhagesVorrapon Chaikeeratisak0Katrina Nguyen1MacKennon E. Egan2Marcella L. Erb3Anastasia Vavilina4Joe Pogliano5Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USADivision of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USADivision of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USADivision of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USADivision of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USADivision of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USAWe recently demonstrated that the large Pseudomonas chlororaphis bacteriophage 201φ2-1 assembles a nucleus-like structure that encloses phage DNA and segregates proteins according to function, with DNA processing proteins inside and metabolic enzymes and ribosomes outside the nucleus. Here, we investigate the replication pathway of the Pseudomonas aeruginosa bacteriophages φKZ and φPA3. Bacteriophages φKZ and φPA3 encode a proteinaceous shell that assembles a nucleus-like structure that compartmentalizes proteins and DNA during viral infection. We show that the tubulin-like protein PhuZ encoded by each phage assembles a bipolar spindle that displays dynamic instability and positions the nucleus at midcell. Our results suggest that the phage spindle and nucleus play the same functional role in all three phages, 201φ2-1, φKZ, and φPA3, demonstrating that these key structures are conserved among large Pseudomonas phages.http://www.sciencedirect.com/science/article/pii/S2211124717310574spindlenucleusphagetubulinPhuZcompartmentalizationnucleus positioningnucleus originviral eukaryogenesis |
| spellingShingle | Vorrapon Chaikeeratisak Katrina Nguyen MacKennon E. Egan Marcella L. Erb Anastasia Vavilina Joe Pogliano The Phage Nucleus and Tubulin Spindle Are Conserved among Large Pseudomonas Phages Cell Reports spindle nucleus phage tubulin PhuZ compartmentalization nucleus positioning nucleus origin viral eukaryogenesis |
| title | The Phage Nucleus and Tubulin Spindle Are Conserved among Large Pseudomonas Phages |
| title_full | The Phage Nucleus and Tubulin Spindle Are Conserved among Large Pseudomonas Phages |
| title_fullStr | The Phage Nucleus and Tubulin Spindle Are Conserved among Large Pseudomonas Phages |
| title_full_unstemmed | The Phage Nucleus and Tubulin Spindle Are Conserved among Large Pseudomonas Phages |
| title_short | The Phage Nucleus and Tubulin Spindle Are Conserved among Large Pseudomonas Phages |
| title_sort | phage nucleus and tubulin spindle are conserved among large pseudomonas phages |
| topic | spindle nucleus phage tubulin PhuZ compartmentalization nucleus positioning nucleus origin viral eukaryogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S2211124717310574 |
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