Induction of Interleukin-6 Trigger an Apoptosis Through Il-17 and Stat3 Pathway that Alleviated by Phycocyanin Treatment
This study was to analyze the optimum dose of IL-6 induction in pregnant rats that could trigger an increase in mean arterial pressure and protein as two symptoms of preeclampsia. A total of 25 pregnant rats was divided into 5 groups, including pregnant rats the control group (without induction of I...
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| Format: | Article |
| Language: | English |
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Research and Community Service Department (LPPM) Universitas Wijaya Kusuma Surabaya
2018-03-01
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| Series: | Jurnal Ilmiah Kedokteran Wijaya Kusuma |
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| Online Access: | https://journal.uwks.ac.id/index.php/jikw/article/view/337 |
| _version_ | 1797715377278418944 |
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| author | Elizabeth Haryanti Harry K Gondo |
| author_facet | Elizabeth Haryanti Harry K Gondo |
| author_sort | Elizabeth Haryanti |
| collection | DOAJ |
| description | This study was to analyze the optimum dose of IL-6 induction in pregnant rats that could trigger an increase in mean arterial pressure and protein as two symptoms of preeclampsia. A total of 25 pregnant rats was divided into 5 groups, including pregnant rats the control group (without induction of IL-6), a group of pregnant rats were given an induction in IL-6 doses of 1.25 ng/day, a group of pregnant rats given doses of IL-6 induction 2.5 ng/day, a group of pregnant rats were given an induction of IL-6 doses of 5 ng/day, and groups of pregnant rats were given an induction in IL-6 doses of 10 ng/day. Induction of IL-6 was performed on the tenth day of gestation for 5 days. The expression of caspase-3, IL-17, and STAT3 were analyzed by confocal laser scanning microscopy. The expression of caspase-3, IL-17 and STAT3 was significantly higher in preeclampsia group than the control group (p<0.05). The expression of caspase-3 decreased significantly in all groups were treated by phycocyanin compared to the preeclamptic group (p<0.05), but has not been able to reach expression comparable to the control group (p<0.05). Expression of IL-17 decreased significantly in the group given the two highest doses of phycocyanin compared to the preeclampsia group (p<0.05). STAT3 expression decreased significantly in all groups were treatedby phycocyanin compared to the preeclamptic group (p<0.05), reached expression comparable to the control group in the group received phycocyanin at doses of 10 and 20 ng (p> 0.05). In conclusion, IL-6 on pregnant rats were able to increase apoptosis through IL-17 and STAT pathway. Inhibition of apoptosis due to phycoyanin treatment not only involve the formation of IL-17, this data is found in phycocyanin doses of 10 and 20 ng. |
| first_indexed | 2024-03-12T08:05:52Z |
| format | Article |
| id | doaj.art-6fdee0b72dc04aeb877309c669cc82d0 |
| institution | Directory Open Access Journal |
| issn | 1978-2071 2580-5967 |
| language | English |
| last_indexed | 2024-03-12T08:05:52Z |
| publishDate | 2018-03-01 |
| publisher | Research and Community Service Department (LPPM) Universitas Wijaya Kusuma Surabaya |
| record_format | Article |
| series | Jurnal Ilmiah Kedokteran Wijaya Kusuma |
| spelling | doaj.art-6fdee0b72dc04aeb877309c669cc82d02023-09-02T19:33:34ZengResearch and Community Service Department (LPPM) Universitas Wijaya Kusuma SurabayaJurnal Ilmiah Kedokteran Wijaya Kusuma1978-20712580-59672018-03-0152610311Induction of Interleukin-6 Trigger an Apoptosis Through Il-17 and Stat3 Pathway that Alleviated by Phycocyanin TreatmentElizabeth Haryanti0Harry K Gondo1Department of Internal Medicine Medical Faculty Universitas Wijaya Kusuma SurabayaDepartment of Obstetrics and Gynecology Medical Faculty Universitas Wijaya Kusuma SurabayaThis study was to analyze the optimum dose of IL-6 induction in pregnant rats that could trigger an increase in mean arterial pressure and protein as two symptoms of preeclampsia. A total of 25 pregnant rats was divided into 5 groups, including pregnant rats the control group (without induction of IL-6), a group of pregnant rats were given an induction in IL-6 doses of 1.25 ng/day, a group of pregnant rats given doses of IL-6 induction 2.5 ng/day, a group of pregnant rats were given an induction of IL-6 doses of 5 ng/day, and groups of pregnant rats were given an induction in IL-6 doses of 10 ng/day. Induction of IL-6 was performed on the tenth day of gestation for 5 days. The expression of caspase-3, IL-17, and STAT3 were analyzed by confocal laser scanning microscopy. The expression of caspase-3, IL-17 and STAT3 was significantly higher in preeclampsia group than the control group (p<0.05). The expression of caspase-3 decreased significantly in all groups were treated by phycocyanin compared to the preeclamptic group (p<0.05), but has not been able to reach expression comparable to the control group (p<0.05). Expression of IL-17 decreased significantly in the group given the two highest doses of phycocyanin compared to the preeclampsia group (p<0.05). STAT3 expression decreased significantly in all groups were treatedby phycocyanin compared to the preeclamptic group (p<0.05), reached expression comparable to the control group in the group received phycocyanin at doses of 10 and 20 ng (p> 0.05). In conclusion, IL-6 on pregnant rats were able to increase apoptosis through IL-17 and STAT pathway. Inhibition of apoptosis due to phycoyanin treatment not only involve the formation of IL-17, this data is found in phycocyanin doses of 10 and 20 ng.https://journal.uwks.ac.id/index.php/jikw/article/view/337preeclampsia modelapoptosisIL-17STAT3rats |
| spellingShingle | Elizabeth Haryanti Harry K Gondo Induction of Interleukin-6 Trigger an Apoptosis Through Il-17 and Stat3 Pathway that Alleviated by Phycocyanin Treatment Jurnal Ilmiah Kedokteran Wijaya Kusuma preeclampsia model apoptosis IL-17 STAT3 rats |
| title | Induction of Interleukin-6 Trigger an Apoptosis Through Il-17 and Stat3 Pathway that Alleviated by Phycocyanin Treatment |
| title_full | Induction of Interleukin-6 Trigger an Apoptosis Through Il-17 and Stat3 Pathway that Alleviated by Phycocyanin Treatment |
| title_fullStr | Induction of Interleukin-6 Trigger an Apoptosis Through Il-17 and Stat3 Pathway that Alleviated by Phycocyanin Treatment |
| title_full_unstemmed | Induction of Interleukin-6 Trigger an Apoptosis Through Il-17 and Stat3 Pathway that Alleviated by Phycocyanin Treatment |
| title_short | Induction of Interleukin-6 Trigger an Apoptosis Through Il-17 and Stat3 Pathway that Alleviated by Phycocyanin Treatment |
| title_sort | induction of interleukin 6 trigger an apoptosis through il 17 and stat3 pathway that alleviated by phycocyanin treatment |
| topic | preeclampsia model apoptosis IL-17 STAT3 rats |
| url | https://journal.uwks.ac.id/index.php/jikw/article/view/337 |
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