Antibiotic Resistance Genes Carried by Commensal <i>Escherichia coli</i> from Shelter Cats in Italy
Antimicrobial resistance is a widespread global health problem. The presence of resistant bacteria and antibiotic resistance genes has been demonstrated not only in humans but also in animals, including pets. Stray cats share the urban environment with people and pets. This may facilitate transmissi...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/12/680 |
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| author | Delia Gambino Francesco Giuseppe Galluzzo Luca Cicero Roberta Cirincione Erika Mannino Veronica Fiore Daniela Proverbio Eva Spada Giovanni Cassata Valeria Gargano |
| author_facet | Delia Gambino Francesco Giuseppe Galluzzo Luca Cicero Roberta Cirincione Erika Mannino Veronica Fiore Daniela Proverbio Eva Spada Giovanni Cassata Valeria Gargano |
| author_sort | Delia Gambino |
| collection | DOAJ |
| description | Antimicrobial resistance is a widespread global health problem. The presence of resistant bacteria and antibiotic resistance genes has been demonstrated not only in humans but also in animals, including pets. Stray cats share the urban environment with people and pets. This may facilitate transmission of resistant bacteria and resistance genes between stray animals, people and domestic animals. Several studies have investigated the role of stray cats as a fecal carrier of ESBL-producing bacteria. However, there are many genes and resistance mechanisms that can be detected in commensal <i>E. coli</i>, which, because of its genetic plasticity, is considered an indicator for monitoring antibiotic resistance. In this study, rectal swabs were collected from stray cats from colonies and shelters in the city of Monza (Monza Brianza, Italy) to isolate commensal <i>E. coli</i>. Phenotypic tests, such as the minimum inhibitory concentration (MIC) and the double disc test (DDST), and molecular analyses to detect antimicrobial resistance genes (ARGs) were used to study the resistance of these isolates. The results obtained confirm that stray cats can carry ESBL-producing <i>E. coli</i> (6.7%) and genes conferring resistance to other important antibiotic classes such as tetracyclines and sulfonamides. |
| first_indexed | 2024-03-08T20:18:09Z |
| format | Article |
| id | doaj.art-6fe0422379034102ab5014c42e217066 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-08T20:18:09Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-6fe0422379034102ab5014c42e2170662023-12-22T14:48:18ZengMDPI AGVeterinary Sciences2306-73812023-11-01101268010.3390/vetsci10120680Antibiotic Resistance Genes Carried by Commensal <i>Escherichia coli</i> from Shelter Cats in ItalyDelia Gambino0Francesco Giuseppe Galluzzo1Luca Cicero2Roberta Cirincione3Erika Mannino4Veronica Fiore5Daniela Proverbio6Eva Spada7Giovanni Cassata8Valeria Gargano9Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, 90129 Palermo, ItalyDepartment of Veterinary Medicine and Animal Sciences (DIVAS), University of Milan, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences (DIVAS), University of Milan, 26900 Lodi, ItalyIstituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, 90129 Palermo, ItalyAntimicrobial resistance is a widespread global health problem. The presence of resistant bacteria and antibiotic resistance genes has been demonstrated not only in humans but also in animals, including pets. Stray cats share the urban environment with people and pets. This may facilitate transmission of resistant bacteria and resistance genes between stray animals, people and domestic animals. Several studies have investigated the role of stray cats as a fecal carrier of ESBL-producing bacteria. However, there are many genes and resistance mechanisms that can be detected in commensal <i>E. coli</i>, which, because of its genetic plasticity, is considered an indicator for monitoring antibiotic resistance. In this study, rectal swabs were collected from stray cats from colonies and shelters in the city of Monza (Monza Brianza, Italy) to isolate commensal <i>E. coli</i>. Phenotypic tests, such as the minimum inhibitory concentration (MIC) and the double disc test (DDST), and molecular analyses to detect antimicrobial resistance genes (ARGs) were used to study the resistance of these isolates. The results obtained confirm that stray cats can carry ESBL-producing <i>E. coli</i> (6.7%) and genes conferring resistance to other important antibiotic classes such as tetracyclines and sulfonamides.https://www.mdpi.com/2306-7381/10/12/680<i>Escherichia coli</i>antimicrobial resistancestray cats |
| spellingShingle | Delia Gambino Francesco Giuseppe Galluzzo Luca Cicero Roberta Cirincione Erika Mannino Veronica Fiore Daniela Proverbio Eva Spada Giovanni Cassata Valeria Gargano Antibiotic Resistance Genes Carried by Commensal <i>Escherichia coli</i> from Shelter Cats in Italy Veterinary Sciences <i>Escherichia coli</i> antimicrobial resistance stray cats |
| title | Antibiotic Resistance Genes Carried by Commensal <i>Escherichia coli</i> from Shelter Cats in Italy |
| title_full | Antibiotic Resistance Genes Carried by Commensal <i>Escherichia coli</i> from Shelter Cats in Italy |
| title_fullStr | Antibiotic Resistance Genes Carried by Commensal <i>Escherichia coli</i> from Shelter Cats in Italy |
| title_full_unstemmed | Antibiotic Resistance Genes Carried by Commensal <i>Escherichia coli</i> from Shelter Cats in Italy |
| title_short | Antibiotic Resistance Genes Carried by Commensal <i>Escherichia coli</i> from Shelter Cats in Italy |
| title_sort | antibiotic resistance genes carried by commensal i escherichia coli i from shelter cats in italy |
| topic | <i>Escherichia coli</i> antimicrobial resistance stray cats |
| url | https://www.mdpi.com/2306-7381/10/12/680 |
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