Microarray expression profile of mRNAs and long noncoding RNAs and the potential role of PFK-1 in infantile hemangioma
Abstract Background Infantile hemangioma (IH) is the most common benign tumor in children. Long noncoding RNAs (lncRNAs) play a critical role in tumorigenesis. However, the expression levels and biological functions of lncRNAs in IH have not been well-studied. This study aimed to analyze the express...
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| Format: | Article |
| Language: | English |
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BMC
2021-01-01
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| Series: | Cell Division |
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| Online Access: | https://doi.org/10.1186/s13008-020-00069-y |
| _version_ | 1811270801521901568 |
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| author | Kaiying Yang Xuepeng Zhang Linwen Chen Siyuan Chen Yi Ji |
| author_facet | Kaiying Yang Xuepeng Zhang Linwen Chen Siyuan Chen Yi Ji |
| author_sort | Kaiying Yang |
| collection | DOAJ |
| description | Abstract Background Infantile hemangioma (IH) is the most common benign tumor in children. Long noncoding RNAs (lncRNAs) play a critical role in tumorigenesis. However, the expression levels and biological functions of lncRNAs in IH have not been well-studied. This study aimed to analyze the expression profile of lncRNAs and mRNAs in proliferating and involuting IHs. Methods The expression profiles of lncRNAs and mRNAs in proliferating and involuting IHs were identified by microarray analysis. Subsequently, detailed bioinformatics analyses were performed. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) analyses were conducted to validate the microarray results. Results In total, 146 differentially expressed (DE) lncRNAs and 374 DE mRNAs were identified. The DE mRNAs were enriched mostly in angiogenesis-related biological processes (BPs) and pathways by bioinformatics analysis. In addition, metabolism-related BPs (e.g., “glycogen biosynthetic process” and “metabolic process”) and pathways (e.g., “oxidative phosphorylation”) were identified. A lncRNA-mRNA co-expression network was constructed from 42 DE lncRNAs and 217 DE mRNAs. Twelve lncRNAs were predicted to have cis-regulated target genes. The microarray analysis results were validated by qRT-PCR using 5 randomly selected lncRNAs and 13 mRNAs. The IHC results revealed that both LOXL2 and FPK-1 exhibited higher protein expression levels in proliferating IH than in involuting IH. Moreover, inhibition of PFK-1 could suppress hemangioma-derived endothelial cell proliferation and migration, induce cell arrest, and reduce glucose uptake and lactate and ATP production. Conclusions The findings suggest that the identified DE lncRNAs and mRNAs may be associated with the pathogenesis of IH. The data presented herein can improve our understanding of IH development and provide direction for further studies investigating the mechanism underlying IH. |
| first_indexed | 2024-04-12T22:08:37Z |
| format | Article |
| id | doaj.art-6fe0ddd5dcf34f4b987c15fadf10403e |
| institution | Directory Open Access Journal |
| issn | 1747-1028 |
| language | English |
| last_indexed | 2024-04-12T22:08:37Z |
| publishDate | 2021-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell Division |
| spelling | doaj.art-6fe0ddd5dcf34f4b987c15fadf10403e2022-12-22T03:14:50ZengBMCCell Division1747-10282021-01-0116111710.1186/s13008-020-00069-yMicroarray expression profile of mRNAs and long noncoding RNAs and the potential role of PFK-1 in infantile hemangiomaKaiying Yang0Xuepeng Zhang1Linwen Chen2Siyuan Chen3Yi Ji4Division of Oncology, Department of Pediatric Surgery, West China Hospital of Sichuan UniversityPediatric Intensive Care Unit, Department of Critical Care Medicine, West China Hospital of Sichuan UniversityCollege of Clinical Medicine, North Sichuan Medical CollegePediatric Intensive Care Unit, Department of Critical Care Medicine, West China Hospital of Sichuan UniversityDivision of Oncology, Department of Pediatric Surgery, West China Hospital of Sichuan UniversityAbstract Background Infantile hemangioma (IH) is the most common benign tumor in children. Long noncoding RNAs (lncRNAs) play a critical role in tumorigenesis. However, the expression levels and biological functions of lncRNAs in IH have not been well-studied. This study aimed to analyze the expression profile of lncRNAs and mRNAs in proliferating and involuting IHs. Methods The expression profiles of lncRNAs and mRNAs in proliferating and involuting IHs were identified by microarray analysis. Subsequently, detailed bioinformatics analyses were performed. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) analyses were conducted to validate the microarray results. Results In total, 146 differentially expressed (DE) lncRNAs and 374 DE mRNAs were identified. The DE mRNAs were enriched mostly in angiogenesis-related biological processes (BPs) and pathways by bioinformatics analysis. In addition, metabolism-related BPs (e.g., “glycogen biosynthetic process” and “metabolic process”) and pathways (e.g., “oxidative phosphorylation”) were identified. A lncRNA-mRNA co-expression network was constructed from 42 DE lncRNAs and 217 DE mRNAs. Twelve lncRNAs were predicted to have cis-regulated target genes. The microarray analysis results were validated by qRT-PCR using 5 randomly selected lncRNAs and 13 mRNAs. The IHC results revealed that both LOXL2 and FPK-1 exhibited higher protein expression levels in proliferating IH than in involuting IH. Moreover, inhibition of PFK-1 could suppress hemangioma-derived endothelial cell proliferation and migration, induce cell arrest, and reduce glucose uptake and lactate and ATP production. Conclusions The findings suggest that the identified DE lncRNAs and mRNAs may be associated with the pathogenesis of IH. The data presented herein can improve our understanding of IH development and provide direction for further studies investigating the mechanism underlying IH.https://doi.org/10.1186/s13008-020-00069-yInfantile hemangiomaLong noncoding RNAmRNAPFK-1MicroarrayBioinformatics analysis |
| spellingShingle | Kaiying Yang Xuepeng Zhang Linwen Chen Siyuan Chen Yi Ji Microarray expression profile of mRNAs and long noncoding RNAs and the potential role of PFK-1 in infantile hemangioma Cell Division Infantile hemangioma Long noncoding RNA mRNA PFK-1 Microarray Bioinformatics analysis |
| title | Microarray expression profile of mRNAs and long noncoding RNAs and the potential role of PFK-1 in infantile hemangioma |
| title_full | Microarray expression profile of mRNAs and long noncoding RNAs and the potential role of PFK-1 in infantile hemangioma |
| title_fullStr | Microarray expression profile of mRNAs and long noncoding RNAs and the potential role of PFK-1 in infantile hemangioma |
| title_full_unstemmed | Microarray expression profile of mRNAs and long noncoding RNAs and the potential role of PFK-1 in infantile hemangioma |
| title_short | Microarray expression profile of mRNAs and long noncoding RNAs and the potential role of PFK-1 in infantile hemangioma |
| title_sort | microarray expression profile of mrnas and long noncoding rnas and the potential role of pfk 1 in infantile hemangioma |
| topic | Infantile hemangioma Long noncoding RNA mRNA PFK-1 Microarray Bioinformatics analysis |
| url | https://doi.org/10.1186/s13008-020-00069-y |
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