Development of Auto-PIVOT: Automated Platform In Vitro for Cell Tissue Culture
Bioreactors are growing in popularity among network engineers. Bioreactors are generally chambers used for cell culture processes with predetermined parameters. Pivot is a bioreactor system for cell culture that is controlled automatically and remotely using multi-chambers that allow inflow of f...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universitas Indonesia
2022-12-01
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| Series: | International Journal of Technology |
| Subjects: | |
| Online Access: | https://ijtech.eng.ui.ac.id/article/view/6176 |
| _version_ | 1797974766394540032 |
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| author | Muhammad Irsyad Yudan Whulanza Puspita Anggraini Katili Radiana Dhewayani Antarianto Chyntia Olivia Maurine Jasirwan Nilo Bugtai |
| author_facet | Muhammad Irsyad Yudan Whulanza Puspita Anggraini Katili Radiana Dhewayani Antarianto Chyntia Olivia Maurine Jasirwan Nilo Bugtai |
| author_sort | Muhammad Irsyad |
| collection | DOAJ |
| description | Bioreactors are growing in popularity among network
engineers. Bioreactors are generally chambers used for cell culture processes
with predetermined parameters. Pivot is a bioreactor system for cell culture
that is controlled automatically and remotely using multi-chambers that allow
inflow of fresh liquid or output for sample collection. This system facilitates
gas exchange between the culture medium and ambient gas. The system provides a
storage bag for fresh media and a peristaltic pump is used to move the media in
a closed loop during perfusion and stirring in the cell culture chamber. The
flow rates used is 2 mL/min. The system has a container for direct sample
measurement to obtain pH, oxygen, and carbon dioxide parameters. The bioreactor
system is also equipped with a mixing vessel which allows the addition of
desired nutrients or additives to the system. The results of a computational
fluid dynamics (CFD) and shear stress-based flow analysis have been conducted.
The simulation results demonstrate that the applied Pivot parameters correspond
to an ideal environment to hepatocyte cell viability and growth. This research
is expected to increase the number of cells produced without reducing the
quality of each chamber and be carried out simultaneously, automatically, and
remotely controlled. |
| first_indexed | 2024-04-11T04:24:57Z |
| format | Article |
| id | doaj.art-6fe10552fe2a43848d8fb609cd5d01bc |
| institution | Directory Open Access Journal |
| issn | 2086-9614 2087-2100 |
| language | English |
| last_indexed | 2024-04-11T04:24:57Z |
| publishDate | 2022-12-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | International Journal of Technology |
| spelling | doaj.art-6fe10552fe2a43848d8fb609cd5d01bc2022-12-30T02:23:43ZengUniversitas IndonesiaInternational Journal of Technology2086-96142087-21002022-12-011381651166210.14716/ijtech.v13i8.61766176Development of Auto-PIVOT: Automated Platform In Vitro for Cell Tissue CultureMuhammad Irsyad0Yudan Whulanza1Puspita Anggraini Katili2Radiana Dhewayani Antarianto3Chyntia Olivia Maurine Jasirwan4Nilo Bugtai5Department of Mechanical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, West Java 16424 Indonesia1. Department of Mechanical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, West Java 16424 Indonesia, 2. Research Center for Biomedical Engineering, Faculty of Engineerin1. Research Center for Biomedical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, West Java 16424 Indonesia, 2. Biomedical Engineering Study Program, Department of ElectriDepartment of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, IndonesiaDivision of Hepatobilliary, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, IndonesiaDepartment of Manufacturing Engineering and Management, Gokongwei College of Engineering, De La Salle University, 1004 Manila, PhilippinesBioreactors are growing in popularity among network engineers. Bioreactors are generally chambers used for cell culture processes with predetermined parameters. Pivot is a bioreactor system for cell culture that is controlled automatically and remotely using multi-chambers that allow inflow of fresh liquid or output for sample collection. This system facilitates gas exchange between the culture medium and ambient gas. The system provides a storage bag for fresh media and a peristaltic pump is used to move the media in a closed loop during perfusion and stirring in the cell culture chamber. The flow rates used is 2 mL/min. The system has a container for direct sample measurement to obtain pH, oxygen, and carbon dioxide parameters. The bioreactor system is also equipped with a mixing vessel which allows the addition of desired nutrients or additives to the system. The results of a computational fluid dynamics (CFD) and shear stress-based flow analysis have been conducted. The simulation results demonstrate that the applied Pivot parameters correspond to an ideal environment to hepatocyte cell viability and growth. This research is expected to increase the number of cells produced without reducing the quality of each chamber and be carried out simultaneously, automatically, and remotely controlled.https://ijtech.eng.ui.ac.id/article/view/6176automated systembioreactorcell culturepivotremotely controlled |
| spellingShingle | Muhammad Irsyad Yudan Whulanza Puspita Anggraini Katili Radiana Dhewayani Antarianto Chyntia Olivia Maurine Jasirwan Nilo Bugtai Development of Auto-PIVOT: Automated Platform In Vitro for Cell Tissue Culture International Journal of Technology automated system bioreactor cell culture pivot remotely controlled |
| title | Development of Auto-PIVOT: Automated Platform In Vitro for Cell Tissue Culture |
| title_full | Development of Auto-PIVOT: Automated Platform In Vitro for Cell Tissue Culture |
| title_fullStr | Development of Auto-PIVOT: Automated Platform In Vitro for Cell Tissue Culture |
| title_full_unstemmed | Development of Auto-PIVOT: Automated Platform In Vitro for Cell Tissue Culture |
| title_short | Development of Auto-PIVOT: Automated Platform In Vitro for Cell Tissue Culture |
| title_sort | development of auto pivot automated platform in vitro for cell tissue culture |
| topic | automated system bioreactor cell culture pivot remotely controlled |
| url | https://ijtech.eng.ui.ac.id/article/view/6176 |
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