Utilizing of alkybenzene homologous series on the determination of kovats retention index in the RP-HPLC using methanol/water solvent system
Retention times in high performance liquid chromatography are very susceptible to small changes of the stationary phase, temperature, pH-value, mobile-phase composition and flow rate. One of the methods to minimize these effects is by utilizing a retention index system. In contrast to GC, the retent...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada
2009-05-01
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| Series: | Indonesian Journal of Pharmacy |
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| Online Access: | http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/496 |
| _version_ | 1818577488963436544 |
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| author | Rinaldi Idroes |
| author_facet | Rinaldi Idroes |
| author_sort | Rinaldi Idroes |
| collection | DOAJ |
| description | Retention times in high performance liquid chromatography are very susceptible to small changes of the stationary phase, temperature, pH-value, mobile-phase composition and flow rate. One of the methods to minimize these effects is by utilizing a retention index system. In contrast to GC, the retention Index determination method in HPLC is still widely discussed, because of difficulty utilizing n-alkane as standard. In addition, the solutes in HPLC interact with the mobile-phase, thus the retention data also depend on the mobilephase. It is not easy to use n-alkane in HPLC as standards because of considerable problems. Due to their very non polar properties, hence in most reversed phase systems, they show large retention times. Therefore, using nalkane in routine analysis could be inconvenient. In comparison with n-alkane, the alkylbenzene homologous series are stable compound and commercially available. They have a high UV absorbance at the wavelength of 254 nm, and thus are easily detected by a UV detector. This paper will introduce Kovats Retention Index Determination in the HPLC by using alkylbenzene homologous series and then is to be connected with n-alkane as a frame of reference. Steroids were used as test substance for determining Kovats retention index values in methanol/ water system. |
| first_indexed | 2024-12-16T06:30:43Z |
| format | Article |
| id | doaj.art-6fe467d747ed4625993dd43eb41f4b94 |
| institution | Directory Open Access Journal |
| issn | 2338-9427 2338-9486 |
| language | English |
| last_indexed | 2024-12-16T06:30:43Z |
| publishDate | 2009-05-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Indonesian Journal of Pharmacy |
| spelling | doaj.art-6fe467d747ed4625993dd43eb41f4b942022-12-21T22:40:54ZengUniversitas Gadjah MadaIndonesian Journal of Pharmacy2338-94272338-94862009-05-01202778310.14499/indonesianjpharm0iss0pp77-83Utilizing of alkybenzene homologous series on the determination of kovats retention index in the RP-HPLC using methanol/water solvent systemRinaldi Idroes 0Jurusan Kimia FMIPA Universitas Syiah Kuala Banda Aceh, Indonesia Retention times in high performance liquid chromatography are very susceptible to small changes of the stationary phase, temperature, pH-value, mobile-phase composition and flow rate. One of the methods to minimize these effects is by utilizing a retention index system. In contrast to GC, the retention Index determination method in HPLC is still widely discussed, because of difficulty utilizing n-alkane as standard. In addition, the solutes in HPLC interact with the mobile-phase, thus the retention data also depend on the mobilephase. It is not easy to use n-alkane in HPLC as standards because of considerable problems. Due to their very non polar properties, hence in most reversed phase systems, they show large retention times. Therefore, using nalkane in routine analysis could be inconvenient. In comparison with n-alkane, the alkylbenzene homologous series are stable compound and commercially available. They have a high UV absorbance at the wavelength of 254 nm, and thus are easily detected by a UV detector. This paper will introduce Kovats Retention Index Determination in the HPLC by using alkylbenzene homologous series and then is to be connected with n-alkane as a frame of reference. Steroids were used as test substance for determining Kovats retention index values in methanol/ water system.http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/496RP-HPLCKovats Retention Indexn-AlkaneAlkyl Benzene. |
| spellingShingle | Rinaldi Idroes Utilizing of alkybenzene homologous series on the determination of kovats retention index in the RP-HPLC using methanol/water solvent system Indonesian Journal of Pharmacy RP-HPLC Kovats Retention Index n-Alkane Alkyl Benzene. |
| title | Utilizing of alkybenzene homologous series on the determination of kovats retention index in the RP-HPLC using methanol/water solvent system |
| title_full | Utilizing of alkybenzene homologous series on the determination of kovats retention index in the RP-HPLC using methanol/water solvent system |
| title_fullStr | Utilizing of alkybenzene homologous series on the determination of kovats retention index in the RP-HPLC using methanol/water solvent system |
| title_full_unstemmed | Utilizing of alkybenzene homologous series on the determination of kovats retention index in the RP-HPLC using methanol/water solvent system |
| title_short | Utilizing of alkybenzene homologous series on the determination of kovats retention index in the RP-HPLC using methanol/water solvent system |
| title_sort | utilizing of alkybenzene homologous series on the determination of kovats retention index in the rp hplc using methanol water solvent system |
| topic | RP-HPLC Kovats Retention Index n-Alkane Alkyl Benzene. |
| url | http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/496 |
| work_keys_str_mv | AT rinaldiidroes utilizingofalkybenzenehomologousseriesonthedeterminationofkovatsretentionindexintherphplcusingmethanolwatersolventsystem |