Genetic diversity and population structure of indigenous guinea fowl (<i>Numida meleagris)</i> in Benin using simple sequence repeat markers
<p>This study aimed to assess the genetic diversity and population structure of 12 guinea fowl phenotypes from three climatic zones (Guinean, Sudano-Guinean and Sudanian) of Benin. A total of 96 adult guinea fowl, aged at least 6 months, were selected for blood sampling. Fragment analysis was...
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| Format: | Article |
| Language: | English |
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Copernicus Publications
2023-07-01
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| Series: | Archives Animal Breeding |
| Online Access: | https://aab.copernicus.org/articles/66/183/2023/aab-66-183-2023.pdf |
| _version_ | 1797780538486947840 |
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| author | B. M. Orounladji B. M. Orounladji V. Houndonougbo M. S. Adido A. E. Soara B. A. Hako Touko C. A. A. M. Chrysostome K. Tozo K. Tozo |
| author_facet | B. M. Orounladji B. M. Orounladji V. Houndonougbo M. S. Adido A. E. Soara B. A. Hako Touko C. A. A. M. Chrysostome K. Tozo K. Tozo |
| author_sort | B. M. Orounladji |
| collection | DOAJ |
| description | <p>This study aimed to assess the genetic diversity and population structure of 12 guinea fowl phenotypes from three climatic zones (Guinean,
Sudano-Guinean and Sudanian) of Benin. A total of 96 adult guinea fowl, aged at least 6 months, were selected for blood sampling. Fragment
analysis was carried out using 17 polymorphic microsatellite markers. The informative marker combinations revealed a total of 83 alleles
across all loci, with an average of 5 alleles per locus and a mean polymorphic information content (PIC) of 0.793. This study showed an observed
heterozygosity of 0.492. The inbreeding coefficient values ranged from <span class="inline-formula">−</span>0.086 in white phenotype to 0.226 in cinnamon, showing a deficit of
heterozygotes, suggesting a moderate inbreeding level. A relatively low population differentiation was observed, with a mean fixation index (Fst)
value of 0.033. The short genetic distances between phenotypes, unlike the strong genetic identities, revealed high genetic proximity between the
12 phenotypes of indigenous guinea fowl in Benin. These data indicate the existence of a single indigenous guinea fowl population with high
intra-population genetic diversity with respect to climatic zones or phenotypes. This study will help in the selection of parental breeding stock
for genetic improvement programs, as well as in the conservation for biodiversity maintenance and sustainable use of the indigenous guinea fowl in
the study zones in Benin.</p> |
| first_indexed | 2024-03-12T23:45:27Z |
| format | Article |
| id | doaj.art-6fe5227eed7740c0be73e232d6cf0c8f |
| institution | Directory Open Access Journal |
| issn | 0003-9438 2363-9822 |
| language | English |
| last_indexed | 2024-03-12T23:45:27Z |
| publishDate | 2023-07-01 |
| publisher | Copernicus Publications |
| record_format | Article |
| series | Archives Animal Breeding |
| spelling | doaj.art-6fe5227eed7740c0be73e232d6cf0c8f2023-07-14T09:34:06ZengCopernicus PublicationsArchives Animal Breeding0003-94382363-98222023-07-016618319510.5194/aab-66-183-2023Genetic diversity and population structure of indigenous guinea fowl (<i>Numida meleagris)</i> in Benin using simple sequence repeat markersB. M. Orounladji0B. M. Orounladji1V. Houndonougbo2M. S. Adido3A. E. Soara4B. A. Hako Touko5C. A. A. M. Chrysostome6K. Tozo7K. Tozo8Centre d'Excellence Régional sur les Sciences Aviaires, Université de Lomé, Lomé, TogoFaculté des Sciences Agronomiques, Université d'Abomey-Calavi, Abomey-Calavi, BeninFaculté des Sciences Agronomiques, Université d'Abomey-Calavi, Abomey-Calavi, BeninDepartment of Animal, Aquaculture and Range Sciences, Sokoine University of Agriculture, Morogoro, TanzaniaDépartement Environnement et Forêts, Institut de l'Environnement et de Recherches Agricoles, Centre National de la Recherche Scientifique et Technologique, Ouagadougou, Burkina FasoBiotechnology and Bioinformatics Research Unit, Department of Animal Science, University of Dschang, Dschang, CameroonFaculté des Sciences Agronomiques, Université d'Abomey-Calavi, Abomey-Calavi, BeninCentre d'Excellence Régional sur les Sciences Aviaires, Université de Lomé, Lomé, TogoFaculté des Sciences, Université de Lomé, Lomé, Togo<p>This study aimed to assess the genetic diversity and population structure of 12 guinea fowl phenotypes from three climatic zones (Guinean, Sudano-Guinean and Sudanian) of Benin. A total of 96 adult guinea fowl, aged at least 6 months, were selected for blood sampling. Fragment analysis was carried out using 17 polymorphic microsatellite markers. The informative marker combinations revealed a total of 83 alleles across all loci, with an average of 5 alleles per locus and a mean polymorphic information content (PIC) of 0.793. This study showed an observed heterozygosity of 0.492. The inbreeding coefficient values ranged from <span class="inline-formula">−</span>0.086 in white phenotype to 0.226 in cinnamon, showing a deficit of heterozygotes, suggesting a moderate inbreeding level. A relatively low population differentiation was observed, with a mean fixation index (Fst) value of 0.033. The short genetic distances between phenotypes, unlike the strong genetic identities, revealed high genetic proximity between the 12 phenotypes of indigenous guinea fowl in Benin. These data indicate the existence of a single indigenous guinea fowl population with high intra-population genetic diversity with respect to climatic zones or phenotypes. This study will help in the selection of parental breeding stock for genetic improvement programs, as well as in the conservation for biodiversity maintenance and sustainable use of the indigenous guinea fowl in the study zones in Benin.</p>https://aab.copernicus.org/articles/66/183/2023/aab-66-183-2023.pdf |
| spellingShingle | B. M. Orounladji B. M. Orounladji V. Houndonougbo M. S. Adido A. E. Soara B. A. Hako Touko C. A. A. M. Chrysostome K. Tozo K. Tozo Genetic diversity and population structure of indigenous guinea fowl (<i>Numida meleagris)</i> in Benin using simple sequence repeat markers Archives Animal Breeding |
| title | Genetic diversity and population structure of indigenous guinea fowl (<i>Numida meleagris)</i> in Benin using simple sequence repeat markers |
| title_full | Genetic diversity and population structure of indigenous guinea fowl (<i>Numida meleagris)</i> in Benin using simple sequence repeat markers |
| title_fullStr | Genetic diversity and population structure of indigenous guinea fowl (<i>Numida meleagris)</i> in Benin using simple sequence repeat markers |
| title_full_unstemmed | Genetic diversity and population structure of indigenous guinea fowl (<i>Numida meleagris)</i> in Benin using simple sequence repeat markers |
| title_short | Genetic diversity and population structure of indigenous guinea fowl (<i>Numida meleagris)</i> in Benin using simple sequence repeat markers |
| title_sort | genetic diversity and population structure of indigenous guinea fowl i numida meleagris i in benin using simple sequence repeat markers |
| url | https://aab.copernicus.org/articles/66/183/2023/aab-66-183-2023.pdf |
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