MicroRNAs are indispensable for reprogramming mouse embryonic fibroblasts into induced stem cell-like cells.
MicroRNAs play a pivotal role in cellular maintenance, proliferation, and differentiation. They have also been implicated to play a key role in disease pathogenesis, and more recently, cellular reprogramming. Certain microRNA clusters can enhance or even directly induce reprogramming, while repressi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3380844?pdf=render |
| _version_ | 1819162849852784640 |
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| author | Byeong-Moo Kim Marc-Christian Thier Sangnam Oh Richard Sherwood Chryssa Kanellopoulou Frank Edenhofer Michael Y Choi |
| author_facet | Byeong-Moo Kim Marc-Christian Thier Sangnam Oh Richard Sherwood Chryssa Kanellopoulou Frank Edenhofer Michael Y Choi |
| author_sort | Byeong-Moo Kim |
| collection | DOAJ |
| description | MicroRNAs play a pivotal role in cellular maintenance, proliferation, and differentiation. They have also been implicated to play a key role in disease pathogenesis, and more recently, cellular reprogramming. Certain microRNA clusters can enhance or even directly induce reprogramming, while repressing key proteins involved in microRNA processing decreases reprogramming efficiency. Although microRNAs clearly play important roles in cellular reprogramming, it remains unknown whether microRNAs are absolutely necessary. We endeavored to answer this fundamental question by attempting to reprogram Dicer-null mouse embryonic fibroblasts (MEFs) that lack almost all functional microRNAs using a defined set of transcription factors. Transduction of reprogramming factors using either lentiviral or piggyBac transposon vector into two, independently derived lines of Dicer-null MEFs failed to produce cells resembling embryonic stem cells (ESCs). However, expression of human Dicer in the Dicer-null MEFs restored their reprogramming potential. Our study demonstrates for the first time that microRNAs are indispensable for dedifferentiation reprogramming. |
| first_indexed | 2024-12-22T17:34:47Z |
| format | Article |
| id | doaj.art-6fe53adfb11845948f2c74dc25ca2054 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T17:34:47Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6fe53adfb11845948f2c74dc25ca20542022-12-21T18:18:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0176e3923910.1371/journal.pone.0039239MicroRNAs are indispensable for reprogramming mouse embryonic fibroblasts into induced stem cell-like cells.Byeong-Moo KimMarc-Christian ThierSangnam OhRichard SherwoodChryssa KanellopoulouFrank EdenhoferMichael Y ChoiMicroRNAs play a pivotal role in cellular maintenance, proliferation, and differentiation. They have also been implicated to play a key role in disease pathogenesis, and more recently, cellular reprogramming. Certain microRNA clusters can enhance or even directly induce reprogramming, while repressing key proteins involved in microRNA processing decreases reprogramming efficiency. Although microRNAs clearly play important roles in cellular reprogramming, it remains unknown whether microRNAs are absolutely necessary. We endeavored to answer this fundamental question by attempting to reprogram Dicer-null mouse embryonic fibroblasts (MEFs) that lack almost all functional microRNAs using a defined set of transcription factors. Transduction of reprogramming factors using either lentiviral or piggyBac transposon vector into two, independently derived lines of Dicer-null MEFs failed to produce cells resembling embryonic stem cells (ESCs). However, expression of human Dicer in the Dicer-null MEFs restored their reprogramming potential. Our study demonstrates for the first time that microRNAs are indispensable for dedifferentiation reprogramming.http://europepmc.org/articles/PMC3380844?pdf=render |
| spellingShingle | Byeong-Moo Kim Marc-Christian Thier Sangnam Oh Richard Sherwood Chryssa Kanellopoulou Frank Edenhofer Michael Y Choi MicroRNAs are indispensable for reprogramming mouse embryonic fibroblasts into induced stem cell-like cells. PLoS ONE |
| title | MicroRNAs are indispensable for reprogramming mouse embryonic fibroblasts into induced stem cell-like cells. |
| title_full | MicroRNAs are indispensable for reprogramming mouse embryonic fibroblasts into induced stem cell-like cells. |
| title_fullStr | MicroRNAs are indispensable for reprogramming mouse embryonic fibroblasts into induced stem cell-like cells. |
| title_full_unstemmed | MicroRNAs are indispensable for reprogramming mouse embryonic fibroblasts into induced stem cell-like cells. |
| title_short | MicroRNAs are indispensable for reprogramming mouse embryonic fibroblasts into induced stem cell-like cells. |
| title_sort | micrornas are indispensable for reprogramming mouse embryonic fibroblasts into induced stem cell like cells |
| url | http://europepmc.org/articles/PMC3380844?pdf=render |
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