Visualization and analysis of whole depot adipose tissue neural innervation
Summary: Little is known about the diversity and function of adipose tissue nerves, due in part to the inability to effectively visualize the tissue’s diverse nerve subtypes and the patterns of innervation across an intact depot. The tools to image and quantify adipose tissue innervation are current...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-10-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004221010956 |
| _version_ | 1818728340438122496 |
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| author | Jake W. Willows Magdalena Blaszkiewicz Amy Lamore Samuel Borer Amanda L. Dubois Emma Garner William P. Breeding Karissa B. Tilbury Andre Khalil Kristy L. Townsend |
| author_facet | Jake W. Willows Magdalena Blaszkiewicz Amy Lamore Samuel Borer Amanda L. Dubois Emma Garner William P. Breeding Karissa B. Tilbury Andre Khalil Kristy L. Townsend |
| author_sort | Jake W. Willows |
| collection | DOAJ |
| description | Summary: Little is known about the diversity and function of adipose tissue nerves, due in part to the inability to effectively visualize the tissue’s diverse nerve subtypes and the patterns of innervation across an intact depot. The tools to image and quantify adipose tissue innervation are currently limited. Here, we present a method of tissue processing that decreases tissue thickness in the z-axis while leaving cells intact for subsequent immunostaining. This was combined with autofluorescence quenching techniques to permit intact whole tissues to be mounted on slides and imaged by confocal microscopy, with a complementary means to perform whole tissue neurite density quantification after capture of tiled z-stack images. Additionally, we demonstrate how to visualize nerve terminals (the neuro-adipose nexus) in intact blocks of adipose tissue without z-depth reduction. We have included examples of data demonstrating nerve subtypes, neurovascular interactions, label-free imaging of collagen, and nerve bundle digital cross-sections. |
| first_indexed | 2024-12-17T22:28:26Z |
| format | Article |
| id | doaj.art-6fe736739327491aae06088e39728da0 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-17T22:28:26Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-6fe736739327491aae06088e39728da02022-12-21T21:30:17ZengElsevieriScience2589-00422021-10-012410103127Visualization and analysis of whole depot adipose tissue neural innervationJake W. Willows0Magdalena Blaszkiewicz1Amy Lamore2Samuel Borer3Amanda L. Dubois4Emma Garner5William P. Breeding6Karissa B. Tilbury7Andre Khalil8Kristy L. Townsend9School of Biology and Ecology, University of Maine, Orono, ME, USA; Department of Neurological Surgery, The Ohio State University, 1014 Biomedical Research Tower, 460 W. 12th Avenue, Columbus, OH, USASchool of Biology and Ecology, University of Maine, Orono, ME, USA; Graduate School of Biomedical Science and Engineering, University of Maine, Orono, ME, USA; Department of Neurological Surgery, The Ohio State University, 1014 Biomedical Research Tower, 460 W. 12th Avenue, Columbus, OH, USASchool of Molecular and Biomedical Sciences, University of Maine, Orono, ME, USASchool of Biology and Ecology, University of Maine, Orono, ME, USASchool of Molecular and Biomedical Sciences, University of Maine, Orono, ME, USASchool of Biology and Ecology, University of Maine, Orono, ME, USADepartment of Chemical and Biomedical Engineering, University of Maine, Orono, ME, USAGraduate School of Biomedical Science and Engineering, University of Maine, Orono, ME, USA; Department of Chemical and Biomedical Engineering, University of Maine, Orono, ME, USAGraduate School of Biomedical Science and Engineering, University of Maine, Orono, ME, USA; Department of Chemical and Biomedical Engineering, University of Maine, Orono, ME, USA; CompuMAINE Laboratory, University of Maine, Orono, ME, USASchool of Biology and Ecology, University of Maine, Orono, ME, USA; Graduate School of Biomedical Science and Engineering, University of Maine, Orono, ME, USA; School of Molecular and Biomedical Sciences, University of Maine, Orono, ME, USA; Department of Chemical and Biomedical Engineering, University of Maine, Orono, ME, USA; Department of Neurological Surgery, The Ohio State University, 1014 Biomedical Research Tower, 460 W. 12th Avenue, Columbus, OH, USA; Corresponding authorSummary: Little is known about the diversity and function of adipose tissue nerves, due in part to the inability to effectively visualize the tissue’s diverse nerve subtypes and the patterns of innervation across an intact depot. The tools to image and quantify adipose tissue innervation are currently limited. Here, we present a method of tissue processing that decreases tissue thickness in the z-axis while leaving cells intact for subsequent immunostaining. This was combined with autofluorescence quenching techniques to permit intact whole tissues to be mounted on slides and imaged by confocal microscopy, with a complementary means to perform whole tissue neurite density quantification after capture of tiled z-stack images. Additionally, we demonstrate how to visualize nerve terminals (the neuro-adipose nexus) in intact blocks of adipose tissue without z-depth reduction. We have included examples of data demonstrating nerve subtypes, neurovascular interactions, label-free imaging of collagen, and nerve bundle digital cross-sections.http://www.sciencedirect.com/science/article/pii/S2589004221010956Cell biologyFunctional aspects of cell biologyMethodology in biological sciences |
| spellingShingle | Jake W. Willows Magdalena Blaszkiewicz Amy Lamore Samuel Borer Amanda L. Dubois Emma Garner William P. Breeding Karissa B. Tilbury Andre Khalil Kristy L. Townsend Visualization and analysis of whole depot adipose tissue neural innervation iScience Cell biology Functional aspects of cell biology Methodology in biological sciences |
| title | Visualization and analysis of whole depot adipose tissue neural innervation |
| title_full | Visualization and analysis of whole depot adipose tissue neural innervation |
| title_fullStr | Visualization and analysis of whole depot adipose tissue neural innervation |
| title_full_unstemmed | Visualization and analysis of whole depot adipose tissue neural innervation |
| title_short | Visualization and analysis of whole depot adipose tissue neural innervation |
| title_sort | visualization and analysis of whole depot adipose tissue neural innervation |
| topic | Cell biology Functional aspects of cell biology Methodology in biological sciences |
| url | http://www.sciencedirect.com/science/article/pii/S2589004221010956 |
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