Cationic Polymer-Coated Magnetic Nanoparticles with Antibacterial Properties: Synthesis and In Vitro Characterization
Uniformly sized magnetite nanoparticles (<i>D</i><sub>n</sub> = 16 nm) were prepared by a thermal decomposition of Fe(III) oleate in octadec-1-ene and stabilized by oleic acid. The particles were coated with Sipomer PAM-200 containing both phosphate and methacrylic groups ava...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/9/1077 |
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| author | Anastasiia B. Shatan Vitalii Patsula Aneta Dydowiczová Kristýna Gunár Nadiia Velychkivska Jiřina Hromádková Eduard Petrovský Daniel Horák |
| author_facet | Anastasiia B. Shatan Vitalii Patsula Aneta Dydowiczová Kristýna Gunár Nadiia Velychkivska Jiřina Hromádková Eduard Petrovský Daniel Horák |
| author_sort | Anastasiia B. Shatan |
| collection | DOAJ |
| description | Uniformly sized magnetite nanoparticles (<i>D</i><sub>n</sub> = 16 nm) were prepared by a thermal decomposition of Fe(III) oleate in octadec-1-ene and stabilized by oleic acid. The particles were coated with Sipomer PAM-200 containing both phosphate and methacrylic groups available for the attachment to the iron oxide and at the same time enabling (co)polymerization of 2-(dimethylamino)ethyl methacrylate and/or 2-<i>tert</i>-butylaminoethyl methacrylate at two molar ratios. The poly[2-(dimethylamino)ethyl methacrylate] (PDMAEMA) and poly[2-(dimethylamino)ethyl methacrylate-<i>co</i>-2-<i>tert</i>-butylaminoethyl methacrylate] [P(DMAEMA-TBAEMA)] polymers and the particles were characterized by <sup>1</sup>H NMR spectroscopy, size-exclusion chromatography, transmission electron microscopy, dynamic light scattering, thermogravimetric analysis, magnetometry, and ATR FTIR and atomic absorption spectroscopy. The antimicrobial effect of cationic polymer-coated magnetite nanoparticles tested on both <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> bacteria was found to be time- and dose-responsive. The P(DMAEMA-TBAEMA)-coated magnetite particles possessed superior biocidal properties compared to those of P(DMAEMA)-coated one. |
| first_indexed | 2024-03-10T07:57:06Z |
| format | Article |
| id | doaj.art-6fe7dbad7c7a4e868f41e11b969c29dc |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T07:57:06Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-6fe7dbad7c7a4e868f41e11b969c29dc2023-11-22T11:45:53ZengMDPI AGAntibiotics2079-63822021-09-01109107710.3390/antibiotics10091077Cationic Polymer-Coated Magnetic Nanoparticles with Antibacterial Properties: Synthesis and In Vitro CharacterizationAnastasiia B. Shatan0Vitalii Patsula1Aneta Dydowiczová2Kristýna Gunár3Nadiia Velychkivska4Jiřina Hromádková5Eduard Petrovský6Daniel Horák7Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 06 Prague 6, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 06 Prague 6, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 06 Prague 6, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 06 Prague 6, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 06 Prague 6, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 06 Prague 6, Czech RepublicInstitute of Geophysics, Czech Academy of Sciences, Boční II/1401, 141 31 Prague 4, Czech RepublicInstitute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 06 Prague 6, Czech RepublicUniformly sized magnetite nanoparticles (<i>D</i><sub>n</sub> = 16 nm) were prepared by a thermal decomposition of Fe(III) oleate in octadec-1-ene and stabilized by oleic acid. The particles were coated with Sipomer PAM-200 containing both phosphate and methacrylic groups available for the attachment to the iron oxide and at the same time enabling (co)polymerization of 2-(dimethylamino)ethyl methacrylate and/or 2-<i>tert</i>-butylaminoethyl methacrylate at two molar ratios. The poly[2-(dimethylamino)ethyl methacrylate] (PDMAEMA) and poly[2-(dimethylamino)ethyl methacrylate-<i>co</i>-2-<i>tert</i>-butylaminoethyl methacrylate] [P(DMAEMA-TBAEMA)] polymers and the particles were characterized by <sup>1</sup>H NMR spectroscopy, size-exclusion chromatography, transmission electron microscopy, dynamic light scattering, thermogravimetric analysis, magnetometry, and ATR FTIR and atomic absorption spectroscopy. The antimicrobial effect of cationic polymer-coated magnetite nanoparticles tested on both <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> bacteria was found to be time- and dose-responsive. The P(DMAEMA-TBAEMA)-coated magnetite particles possessed superior biocidal properties compared to those of P(DMAEMA)-coated one.https://www.mdpi.com/2079-6382/10/9/1077magneticnanoparticlesantibacterial activity2-(dimethylamino)ethyl methacrylate2-<i>tert</i>-butylaminoethyl methacrylate |
| spellingShingle | Anastasiia B. Shatan Vitalii Patsula Aneta Dydowiczová Kristýna Gunár Nadiia Velychkivska Jiřina Hromádková Eduard Petrovský Daniel Horák Cationic Polymer-Coated Magnetic Nanoparticles with Antibacterial Properties: Synthesis and In Vitro Characterization Antibiotics magnetic nanoparticles antibacterial activity 2-(dimethylamino)ethyl methacrylate 2-<i>tert</i>-butylaminoethyl methacrylate |
| title | Cationic Polymer-Coated Magnetic Nanoparticles with Antibacterial Properties: Synthesis and In Vitro Characterization |
| title_full | Cationic Polymer-Coated Magnetic Nanoparticles with Antibacterial Properties: Synthesis and In Vitro Characterization |
| title_fullStr | Cationic Polymer-Coated Magnetic Nanoparticles with Antibacterial Properties: Synthesis and In Vitro Characterization |
| title_full_unstemmed | Cationic Polymer-Coated Magnetic Nanoparticles with Antibacterial Properties: Synthesis and In Vitro Characterization |
| title_short | Cationic Polymer-Coated Magnetic Nanoparticles with Antibacterial Properties: Synthesis and In Vitro Characterization |
| title_sort | cationic polymer coated magnetic nanoparticles with antibacterial properties synthesis and in vitro characterization |
| topic | magnetic nanoparticles antibacterial activity 2-(dimethylamino)ethyl methacrylate 2-<i>tert</i>-butylaminoethyl methacrylate |
| url | https://www.mdpi.com/2079-6382/10/9/1077 |
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