Somatic Primary piRNA Biogenesis Driven by cis-Acting RNA Elements and trans-Acting Yb
Primary piRNAs in Drosophila ovarian somatic cells arise from piRNA cluster transcripts and the 3′ UTRs of a subset of mRNAs, including Traffic jam (Tj) mRNA. However, it is unclear how these RNAs are determined as primary piRNA sources. Here, we identify a cis-acting 100-nt fragment in the Tj 3′ UT...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-07-01
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| Series: | Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124715006476 |
| _version_ | 1818313293964509184 |
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| author | Hirotsugu Ishizu Yuka W. Iwasaki Shigeki Hirakata Haruka Ozaki Wataru Iwasaki Haruhiko Siomi Mikiko C. Siomi |
| author_facet | Hirotsugu Ishizu Yuka W. Iwasaki Shigeki Hirakata Haruka Ozaki Wataru Iwasaki Haruhiko Siomi Mikiko C. Siomi |
| author_sort | Hirotsugu Ishizu |
| collection | DOAJ |
| description | Primary piRNAs in Drosophila ovarian somatic cells arise from piRNA cluster transcripts and the 3′ UTRs of a subset of mRNAs, including Traffic jam (Tj) mRNA. However, it is unclear how these RNAs are determined as primary piRNA sources. Here, we identify a cis-acting 100-nt fragment in the Tj 3′ UTR that is sufficient for producing artificial piRNAs from unintegrated DNA. These artificial piRNAs were effective in endogenous gene transcriptional silencing. Yb, a core component of primary piRNA biogenesis center Yb bodies, directly bound the Tj-cis element. Disruption of this interaction markedly reduced piRNA production. Thus, Yb is the trans-acting partner of the Tj-cis element. Yb-CLIP revealed that Yb binding correlated with somatic piRNA production but Tj-cis element downstream sequences produced few artificial piRNAs. We thus propose that Yb determines primary piRNA sources through two modes of action: primary binding to cis elements to specify substrates and secondary binding to downstream regions to increase diversity in piRNA populations. |
| first_indexed | 2024-12-13T08:31:27Z |
| format | Article |
| id | doaj.art-6fe840c429434b18ba1ef4c962f0d129 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-13T08:31:27Z |
| publishDate | 2015-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-6fe840c429434b18ba1ef4c962f0d1292022-12-21T23:53:45ZengElsevierCell Reports2211-12472015-07-0112342944010.1016/j.celrep.2015.06.035Somatic Primary piRNA Biogenesis Driven by cis-Acting RNA Elements and trans-Acting YbHirotsugu Ishizu0Yuka W. Iwasaki1Shigeki Hirakata2Haruka Ozaki3Wataru Iwasaki4Haruhiko Siomi5Mikiko C. Siomi6Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo 113-0032, JapanDepartment of Molecular Biology, Keio University School of Medicine, Tokyo 160-8582, JapanDepartment of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo 113-0032, JapanDepartment of Computational Biology, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo 113-0032, JapanDepartment of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo 113-0032, JapanDepartment of Molecular Biology, Keio University School of Medicine, Tokyo 160-8582, JapanDepartment of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo 113-0032, JapanPrimary piRNAs in Drosophila ovarian somatic cells arise from piRNA cluster transcripts and the 3′ UTRs of a subset of mRNAs, including Traffic jam (Tj) mRNA. However, it is unclear how these RNAs are determined as primary piRNA sources. Here, we identify a cis-acting 100-nt fragment in the Tj 3′ UTR that is sufficient for producing artificial piRNAs from unintegrated DNA. These artificial piRNAs were effective in endogenous gene transcriptional silencing. Yb, a core component of primary piRNA biogenesis center Yb bodies, directly bound the Tj-cis element. Disruption of this interaction markedly reduced piRNA production. Thus, Yb is the trans-acting partner of the Tj-cis element. Yb-CLIP revealed that Yb binding correlated with somatic piRNA production but Tj-cis element downstream sequences produced few artificial piRNAs. We thus propose that Yb determines primary piRNA sources through two modes of action: primary binding to cis elements to specify substrates and secondary binding to downstream regions to increase diversity in piRNA populations.http://www.sciencedirect.com/science/article/pii/S2211124715006476 |
| spellingShingle | Hirotsugu Ishizu Yuka W. Iwasaki Shigeki Hirakata Haruka Ozaki Wataru Iwasaki Haruhiko Siomi Mikiko C. Siomi Somatic Primary piRNA Biogenesis Driven by cis-Acting RNA Elements and trans-Acting Yb Cell Reports |
| title | Somatic Primary piRNA Biogenesis Driven by cis-Acting RNA Elements and trans-Acting Yb |
| title_full | Somatic Primary piRNA Biogenesis Driven by cis-Acting RNA Elements and trans-Acting Yb |
| title_fullStr | Somatic Primary piRNA Biogenesis Driven by cis-Acting RNA Elements and trans-Acting Yb |
| title_full_unstemmed | Somatic Primary piRNA Biogenesis Driven by cis-Acting RNA Elements and trans-Acting Yb |
| title_short | Somatic Primary piRNA Biogenesis Driven by cis-Acting RNA Elements and trans-Acting Yb |
| title_sort | somatic primary pirna biogenesis driven by cis acting rna elements and trans acting yb |
| url | http://www.sciencedirect.com/science/article/pii/S2211124715006476 |
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