SUPER STARCHY1/ONAC025 participates in rice grain filling
Abstract NAC transcription factors (TFs) are known for their role in development and stress. This article attempts to functionally validate the role of rice SS1/ ONAC025 (LOC_Os11g31330) during seed development. The gene is seed‐specific and its promoter directs reporter expression in the developing...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-09-01
|
| Series: | Plant Direct |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/pld3.249 |
| _version_ | 1819094394401193984 |
|---|---|
| author | Iny Elizebeth Mathew Richa Priyadarshini Arunima Mahto Priya Jaiswal Swarup K. Parida Pinky Agarwal |
| author_facet | Iny Elizebeth Mathew Richa Priyadarshini Arunima Mahto Priya Jaiswal Swarup K. Parida Pinky Agarwal |
| author_sort | Iny Elizebeth Mathew |
| collection | DOAJ |
| description | Abstract NAC transcription factors (TFs) are known for their role in development and stress. This article attempts to functionally validate the role of rice SS1/ ONAC025 (LOC_Os11g31330) during seed development. The gene is seed‐specific and its promoter directs reporter expression in the developing endosperm and embryo in rice transgenic plants. Furthermore, rice transgenic plants ectopically expressing SS1/ ONAC025 have a plantlet lethal phenotype with hampered vegetative growth, but increased tillers and an altered shoot apical meristem structure. The vegetative cells of these plantlets are filled with distinct starch granules. RNAseq analysis of two independent plantlets reveals the differential expression of reproductive and photosynthetic genes. A comparison with seed development transcriptome indicates differential regulation of many seed‐related genes by SS1/ ONAC025. Genes involved in starch biosynthesis, especially amylopectin and those encoding seed storage proteins, and regulating seed size are also differentially expressed. In conjunction, SS1/ ONAC025 shows highest expression in japonica rice. As a TF, SS1/ ONAC025 is a transcriptional repressor localized to endoplasmic reticulum and nucleus. The article shows that SS1/ ONAC025 is a seed‐specific gene promoting grain filling in rice, and negatively affecting vegetative growth. |
| first_indexed | 2024-12-21T23:26:43Z |
| format | Article |
| id | doaj.art-6fe9d8ac58684d6ebfc88546bfe54090 |
| institution | Directory Open Access Journal |
| issn | 2475-4455 |
| language | English |
| last_indexed | 2024-12-21T23:26:43Z |
| publishDate | 2020-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj.art-6fe9d8ac58684d6ebfc88546bfe540902022-12-21T18:46:36ZengWileyPlant Direct2475-44552020-09-0149n/an/a10.1002/pld3.249SUPER STARCHY1/ONAC025 participates in rice grain fillingIny Elizebeth Mathew0Richa Priyadarshini1Arunima Mahto2Priya Jaiswal3Swarup K. Parida4Pinky Agarwal5National Institute of Plant Genome Research New Delhi IndiaNational Institute of Plant Genome Research New Delhi IndiaNational Institute of Plant Genome Research New Delhi IndiaNational Institute of Plant Genome Research New Delhi IndiaNational Institute of Plant Genome Research New Delhi IndiaNational Institute of Plant Genome Research New Delhi IndiaAbstract NAC transcription factors (TFs) are known for their role in development and stress. This article attempts to functionally validate the role of rice SS1/ ONAC025 (LOC_Os11g31330) during seed development. The gene is seed‐specific and its promoter directs reporter expression in the developing endosperm and embryo in rice transgenic plants. Furthermore, rice transgenic plants ectopically expressing SS1/ ONAC025 have a plantlet lethal phenotype with hampered vegetative growth, but increased tillers and an altered shoot apical meristem structure. The vegetative cells of these plantlets are filled with distinct starch granules. RNAseq analysis of two independent plantlets reveals the differential expression of reproductive and photosynthetic genes. A comparison with seed development transcriptome indicates differential regulation of many seed‐related genes by SS1/ ONAC025. Genes involved in starch biosynthesis, especially amylopectin and those encoding seed storage proteins, and regulating seed size are also differentially expressed. In conjunction, SS1/ ONAC025 shows highest expression in japonica rice. As a TF, SS1/ ONAC025 is a transcriptional repressor localized to endoplasmic reticulum and nucleus. The article shows that SS1/ ONAC025 is a seed‐specific gene promoting grain filling in rice, and negatively affecting vegetative growth.https://doi.org/10.1002/pld3.249amylopectingrainNACriceseed developmenttranscription factor |
| spellingShingle | Iny Elizebeth Mathew Richa Priyadarshini Arunima Mahto Priya Jaiswal Swarup K. Parida Pinky Agarwal SUPER STARCHY1/ONAC025 participates in rice grain filling Plant Direct amylopectin grain NAC rice seed development transcription factor |
| title | SUPER STARCHY1/ONAC025 participates in rice grain filling |
| title_full | SUPER STARCHY1/ONAC025 participates in rice grain filling |
| title_fullStr | SUPER STARCHY1/ONAC025 participates in rice grain filling |
| title_full_unstemmed | SUPER STARCHY1/ONAC025 participates in rice grain filling |
| title_short | SUPER STARCHY1/ONAC025 participates in rice grain filling |
| title_sort | super starchy1 onac025 participates in rice grain filling |
| topic | amylopectin grain NAC rice seed development transcription factor |
| url | https://doi.org/10.1002/pld3.249 |
| work_keys_str_mv | AT inyelizebethmathew superstarchy1onac025participatesinricegrainfilling AT richapriyadarshini superstarchy1onac025participatesinricegrainfilling AT arunimamahto superstarchy1onac025participatesinricegrainfilling AT priyajaiswal superstarchy1onac025participatesinricegrainfilling AT swarupkparida superstarchy1onac025participatesinricegrainfilling AT pinkyagarwal superstarchy1onac025participatesinricegrainfilling |