Simultaneous detection of 22 mycotoxins in grape by QuEChERS and ultra-high-performance liquid chromatography coupled with quadrupole/orbitrap high-resolution mass spectrometry
ABSTRACT: To effectively assess the extent of the mycotoxin contamination of grapes, a method was developed to analyze 22 mycotoxins by ultra-high-performance liquid chromatography coupled with quadrupole/orbitrap high-resolution mass spectrometry (UPLC-Q-Orbitrap HRMS). Samples were extracted and p...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co. Ltd.
2024-12-01
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| Series: | Journal of Future Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772566923000927 |
| _version_ | 1827582634529652736 |
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| author | Meirong Cao Jing Wang Mengtian Wang Xiaoxuan Yuan Xu Zhang Junzhan Ma Yan Zhang Qiang Li |
| author_facet | Meirong Cao Jing Wang Mengtian Wang Xiaoxuan Yuan Xu Zhang Junzhan Ma Yan Zhang Qiang Li |
| author_sort | Meirong Cao |
| collection | DOAJ |
| description | ABSTRACT: To effectively assess the extent of the mycotoxin contamination of grapes, a method was developed to analyze 22 mycotoxins by ultra-high-performance liquid chromatography coupled with quadrupole/orbitrap high-resolution mass spectrometry (UPLC-Q-Orbitrap HRMS). Samples were extracted and purified using the QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure. The extracts were separated on a BEH Shield C18 column (100 mm × 2.1 mm, 1.7 μm), using methanol and water with 0.1% formic acid solution and 2 mmol/L ammonium acetate as the mobile phases. The quantification was performed according to a matrix-matched external standard method. There was a good linear relationship within the respective mass concentration ranges of 22 mycotoxins; the correlation coefficients were not less than 0.998 1. The limit of quantification was 0.3–10.0 μg/kg. Mycotoxin recovery ranged from 68.6% to 109.0%, with a relative standard deviation of 1.28%–12.80%. The method is simple, rapid, highly sensitive, and accurate, making it useful for screening grapes for common and emerging mycotoxins. |
| first_indexed | 2024-03-08T22:55:31Z |
| format | Article |
| id | doaj.art-6fefe9060365479a98ef07ebe13366c2 |
| institution | Directory Open Access Journal |
| issn | 2772-5669 |
| language | English |
| last_indexed | 2024-03-08T22:55:31Z |
| publishDate | 2024-12-01 |
| publisher | KeAi Communications Co. Ltd. |
| record_format | Article |
| series | Journal of Future Foods |
| spelling | doaj.art-6fefe9060365479a98ef07ebe13366c22023-12-16T06:11:16ZengKeAi Communications Co. Ltd.Journal of Future Foods2772-56692024-12-0144369375Simultaneous detection of 22 mycotoxins in grape by QuEChERS and ultra-high-performance liquid chromatography coupled with quadrupole/orbitrap high-resolution mass spectrometryMeirong Cao0Jing Wang1Mengtian Wang2Xiaoxuan Yuan3Xu Zhang4Junzhan Ma5Yan Zhang6Qiang Li7Hebei Food Safety Key Laboratory, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Engineering Research Center for Special Food Safety and Health, Hebei Food Inspection and Research Institute, Shijiazhuang 050227, ChinaHebei Food Safety Key Laboratory, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Engineering Research Center for Special Food Safety and Health, Hebei Food Inspection and Research Institute, Shijiazhuang 050227, ChinaHebei Key Laboratory of Forensic Medicine, College of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, ChinaHebei Food Safety Key Laboratory, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Engineering Research Center for Special Food Safety and Health, Hebei Food Inspection and Research Institute, Shijiazhuang 050227, ChinaHebei Food Safety Key Laboratory, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Engineering Research Center for Special Food Safety and Health, Hebei Food Inspection and Research Institute, Shijiazhuang 050227, ChinaHebei Food Safety Key Laboratory, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Engineering Research Center for Special Food Safety and Health, Hebei Food Inspection and Research Institute, Shijiazhuang 050227, ChinaHebei Food Safety Key Laboratory, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Engineering Research Center for Special Food Safety and Health, Hebei Food Inspection and Research Institute, Shijiazhuang 050227, China; Hebei Key Laboratory of Forensic Medicine, College of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, ChinaHebei Food Safety Key Laboratory, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Engineering Research Center for Special Food Safety and Health, Hebei Food Inspection and Research Institute, Shijiazhuang 050227, ChinaABSTRACT: To effectively assess the extent of the mycotoxin contamination of grapes, a method was developed to analyze 22 mycotoxins by ultra-high-performance liquid chromatography coupled with quadrupole/orbitrap high-resolution mass spectrometry (UPLC-Q-Orbitrap HRMS). Samples were extracted and purified using the QuEChERS (quick, easy, cheap, effective, rugged, and safe) procedure. The extracts were separated on a BEH Shield C18 column (100 mm × 2.1 mm, 1.7 μm), using methanol and water with 0.1% formic acid solution and 2 mmol/L ammonium acetate as the mobile phases. The quantification was performed according to a matrix-matched external standard method. There was a good linear relationship within the respective mass concentration ranges of 22 mycotoxins; the correlation coefficients were not less than 0.998 1. The limit of quantification was 0.3–10.0 μg/kg. Mycotoxin recovery ranged from 68.6% to 109.0%, with a relative standard deviation of 1.28%–12.80%. The method is simple, rapid, highly sensitive, and accurate, making it useful for screening grapes for common and emerging mycotoxins.http://www.sciencedirect.com/science/article/pii/S2772566923000927MycotoxinsUltra-high-performance liquid chromatography-quadrupole/orbitrap high-resolution mass spectrometryGrape |
| spellingShingle | Meirong Cao Jing Wang Mengtian Wang Xiaoxuan Yuan Xu Zhang Junzhan Ma Yan Zhang Qiang Li Simultaneous detection of 22 mycotoxins in grape by QuEChERS and ultra-high-performance liquid chromatography coupled with quadrupole/orbitrap high-resolution mass spectrometry Journal of Future Foods Mycotoxins Ultra-high-performance liquid chromatography-quadrupole/orbitrap high-resolution mass spectrometry Grape |
| title | Simultaneous detection of 22 mycotoxins in grape by QuEChERS and ultra-high-performance liquid chromatography coupled with quadrupole/orbitrap high-resolution mass spectrometry |
| title_full | Simultaneous detection of 22 mycotoxins in grape by QuEChERS and ultra-high-performance liquid chromatography coupled with quadrupole/orbitrap high-resolution mass spectrometry |
| title_fullStr | Simultaneous detection of 22 mycotoxins in grape by QuEChERS and ultra-high-performance liquid chromatography coupled with quadrupole/orbitrap high-resolution mass spectrometry |
| title_full_unstemmed | Simultaneous detection of 22 mycotoxins in grape by QuEChERS and ultra-high-performance liquid chromatography coupled with quadrupole/orbitrap high-resolution mass spectrometry |
| title_short | Simultaneous detection of 22 mycotoxins in grape by QuEChERS and ultra-high-performance liquid chromatography coupled with quadrupole/orbitrap high-resolution mass spectrometry |
| title_sort | simultaneous detection of 22 mycotoxins in grape by quechers and ultra high performance liquid chromatography coupled with quadrupole orbitrap high resolution mass spectrometry |
| topic | Mycotoxins Ultra-high-performance liquid chromatography-quadrupole/orbitrap high-resolution mass spectrometry Grape |
| url | http://www.sciencedirect.com/science/article/pii/S2772566923000927 |
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