Tryptophan-Dependent Production of Indole-3-Acetic Acid (IAA) Affects Level of Plant Growth Promotion by Bacillus amyloliquefaciens FZB42
Phytohormone-like acting compounds previously have been suggested to be involved in the phytostimulatory action exerted by the plant-beneficial rhizobacterium Bacillus amyloliquefaciens FZB42. Analyses by high-performance liquid chromatography and gas chromatography-mass spectrometry performed with...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2007-06-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-6-0619 |
| _version_ | 1811261301877374976 |
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| author | ElSorra E. Idris Domingo J. Iglesias Manuel Talon Rainer Borriss |
| author_facet | ElSorra E. Idris Domingo J. Iglesias Manuel Talon Rainer Borriss |
| author_sort | ElSorra E. Idris |
| collection | DOAJ |
| description | Phytohormone-like acting compounds previously have been suggested to be involved in the phytostimulatory action exerted by the plant-beneficial rhizobacterium Bacillus amyloliquefaciens FZB42. Analyses by high-performance liquid chromatography and gas chromatography-mass spectrometry performed with culture filtrates of FZB42 demonstrated the presence of indole-3-acetic acid (IAA), corroborating it as one of the pivotal plant-growth-promoting substances produced by this bacterium. In the presence of 5 mM tryptophan, a fivefold increase in IAA secretion was registered. In addition, in the trp auxotrophic strains E101 (ΔtrpBA) and E102 (ΔtrpED), and in two other strains bearing knockout mutations in genes probably involved in IAA metabolism, E103 (ΔysnE, putative IAA transacetylase) and E105 (ΔyhcX, putative nitrilase), the concentration of IAA in the culture filtrates was diminished. Three of these mutant strains were less efficient in promoting plant growth, indicating that the Trp-dependent synthesis of auxins and plant growth promotion are functionally related in B. amyloliquefaciens. |
| first_indexed | 2024-04-12T19:01:04Z |
| format | Article |
| id | doaj.art-6ff32f40c5514b2e9d4fa9c72cae8892 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T19:01:04Z |
| publishDate | 2007-06-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-6ff32f40c5514b2e9d4fa9c72cae88922022-12-22T03:20:08ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062007-06-0120661962610.1094/MPMI-20-6-0619Tryptophan-Dependent Production of Indole-3-Acetic Acid (IAA) Affects Level of Plant Growth Promotion by Bacillus amyloliquefaciens FZB42ElSorra E. IdrisDomingo J. IglesiasManuel TalonRainer BorrissPhytohormone-like acting compounds previously have been suggested to be involved in the phytostimulatory action exerted by the plant-beneficial rhizobacterium Bacillus amyloliquefaciens FZB42. Analyses by high-performance liquid chromatography and gas chromatography-mass spectrometry performed with culture filtrates of FZB42 demonstrated the presence of indole-3-acetic acid (IAA), corroborating it as one of the pivotal plant-growth-promoting substances produced by this bacterium. In the presence of 5 mM tryptophan, a fivefold increase in IAA secretion was registered. In addition, in the trp auxotrophic strains E101 (ΔtrpBA) and E102 (ΔtrpED), and in two other strains bearing knockout mutations in genes probably involved in IAA metabolism, E103 (ΔysnE, putative IAA transacetylase) and E105 (ΔyhcX, putative nitrilase), the concentration of IAA in the culture filtrates was diminished. Three of these mutant strains were less efficient in promoting plant growth, indicating that the Trp-dependent synthesis of auxins and plant growth promotion are functionally related in B. amyloliquefaciens.https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-6-0619 |
| spellingShingle | ElSorra E. Idris Domingo J. Iglesias Manuel Talon Rainer Borriss Tryptophan-Dependent Production of Indole-3-Acetic Acid (IAA) Affects Level of Plant Growth Promotion by Bacillus amyloliquefaciens FZB42 Molecular Plant-Microbe Interactions |
| title | Tryptophan-Dependent Production of Indole-3-Acetic Acid (IAA) Affects Level of Plant Growth Promotion by Bacillus amyloliquefaciens FZB42 |
| title_full | Tryptophan-Dependent Production of Indole-3-Acetic Acid (IAA) Affects Level of Plant Growth Promotion by Bacillus amyloliquefaciens FZB42 |
| title_fullStr | Tryptophan-Dependent Production of Indole-3-Acetic Acid (IAA) Affects Level of Plant Growth Promotion by Bacillus amyloliquefaciens FZB42 |
| title_full_unstemmed | Tryptophan-Dependent Production of Indole-3-Acetic Acid (IAA) Affects Level of Plant Growth Promotion by Bacillus amyloliquefaciens FZB42 |
| title_short | Tryptophan-Dependent Production of Indole-3-Acetic Acid (IAA) Affects Level of Plant Growth Promotion by Bacillus amyloliquefaciens FZB42 |
| title_sort | tryptophan dependent production of indole 3 acetic acid iaa affects level of plant growth promotion by bacillus amyloliquefaciens fzb42 |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-20-6-0619 |
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