Characterization and Neuroprotection Potential of Seleno-Polymannuronate
Seleno-polymannuronate (Se-PM) was prepared from alginate-derived polymannuronate (PM) through a sulfation followed by a selenylation replacement reaction. The organic selenium content of Se-PM was 437.25 μg/g and its average molecular weight was 2.36 kDa. The neuroprotection effect of Se-PM and cor...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-02-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2020.00021/full |
| _version_ | 1818572455730479104 |
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| author | Decheng Bi Xiaofan Li Tong Li Xiuting Li Zhijian Lin Lijun Yao Hui Li Hong Xu Zhangli Hu Zhenqing Zhang Qiong Liu Xu Xu |
| author_facet | Decheng Bi Xiaofan Li Tong Li Xiuting Li Zhijian Lin Lijun Yao Hui Li Hong Xu Zhangli Hu Zhenqing Zhang Qiong Liu Xu Xu |
| author_sort | Decheng Bi |
| collection | DOAJ |
| description | Seleno-polymannuronate (Se-PM) was prepared from alginate-derived polymannuronate (PM) through a sulfation followed by a selenylation replacement reaction. The organic selenium content of Se-PM was 437.25 μg/g and its average molecular weight was 2.36 kDa. The neuroprotection effect of Se-PM and corresponding molecular mechanisms were investigated. Our results showed that, comparing to both sulfated PM (S-PM) and PM, Se-PM remarkably inhibited the aggregation of Aβ1–42 oligomer in vitro and significantly reduced the APP and BACE1 protein expression in N2a-sw cells, highlighting the critical function of the selenium presented in Se-PM. Moreover, Se-PM decreased the expression of cytochrome c and the ratio of Bax to Bcl-2, and enhanced the mitochondrial membrane potential in N2a-sw cells. These results suggested that Se-PM treatment can markedly inhibit N2a-sw cell apoptosis and promote N2a-sw cell survival and that Se-PM might be a potential therapeutic agent for the prevention of neurodegeneration owing to its remarkable neuroprotection effect. |
| first_indexed | 2024-12-14T18:57:25Z |
| format | Article |
| id | doaj.art-6ff4f9a4bdd14e639bbb5d60edc1b9be |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-14T18:57:25Z |
| publishDate | 2020-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-6ff4f9a4bdd14e639bbb5d60edc1b9be2022-12-21T22:51:03ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122020-02-011110.3389/fphar.2020.00021513715Characterization and Neuroprotection Potential of Seleno-PolymannuronateDecheng Bi0Xiaofan Li1Tong Li2Xiuting Li3Zhijian Lin4Lijun Yao5Hui Li6Hong Xu7Zhangli Hu8Zhenqing Zhang9Qiong Liu10Xu Xu11Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing, ChinaDepartment of Neurology, Peking University Shenzhen Hospital, Shenzhen, ChinaShenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaCollege of Pharmaceutical Sciences, Soochow University, Suzhou, ChinaShenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaSeleno-polymannuronate (Se-PM) was prepared from alginate-derived polymannuronate (PM) through a sulfation followed by a selenylation replacement reaction. The organic selenium content of Se-PM was 437.25 μg/g and its average molecular weight was 2.36 kDa. The neuroprotection effect of Se-PM and corresponding molecular mechanisms were investigated. Our results showed that, comparing to both sulfated PM (S-PM) and PM, Se-PM remarkably inhibited the aggregation of Aβ1–42 oligomer in vitro and significantly reduced the APP and BACE1 protein expression in N2a-sw cells, highlighting the critical function of the selenium presented in Se-PM. Moreover, Se-PM decreased the expression of cytochrome c and the ratio of Bax to Bcl-2, and enhanced the mitochondrial membrane potential in N2a-sw cells. These results suggested that Se-PM treatment can markedly inhibit N2a-sw cell apoptosis and promote N2a-sw cell survival and that Se-PM might be a potential therapeutic agent for the prevention of neurodegeneration owing to its remarkable neuroprotection effect.https://www.frontiersin.org/article/10.3389/fphar.2020.00021/fullalginateseleno-polymannuronateanti-apoptosisneuroprotectionN2a-sw cells |
| spellingShingle | Decheng Bi Xiaofan Li Tong Li Xiuting Li Zhijian Lin Lijun Yao Hui Li Hong Xu Zhangli Hu Zhenqing Zhang Qiong Liu Xu Xu Characterization and Neuroprotection Potential of Seleno-Polymannuronate Frontiers in Pharmacology alginate seleno-polymannuronate anti-apoptosis neuroprotection N2a-sw cells |
| title | Characterization and Neuroprotection Potential of Seleno-Polymannuronate |
| title_full | Characterization and Neuroprotection Potential of Seleno-Polymannuronate |
| title_fullStr | Characterization and Neuroprotection Potential of Seleno-Polymannuronate |
| title_full_unstemmed | Characterization and Neuroprotection Potential of Seleno-Polymannuronate |
| title_short | Characterization and Neuroprotection Potential of Seleno-Polymannuronate |
| title_sort | characterization and neuroprotection potential of seleno polymannuronate |
| topic | alginate seleno-polymannuronate anti-apoptosis neuroprotection N2a-sw cells |
| url | https://www.frontiersin.org/article/10.3389/fphar.2020.00021/full |
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