Polydopamine-Coated Co<sub>3</sub>O<sub>4</sub> Nanoparticles as an Efficient Catalase Mimic for Fluorescent Detection of Sulfide Ion
Surface engineering of nanozymes has been recognized as a potent strategy to improve their catalytic activity and specificity. We synthesized polydopamine-coated Co<sub>3</sub>O<sub>4</sub> nanoparticles (PDA@Co<sub>3</sub>O<sub>4</sub> NPs) through si...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/11/1047 |
| _version_ | 1797465778058952704 |
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| author | Trung Hieu Vu Phuong Thy Nguyen Moon Il Kim |
| author_facet | Trung Hieu Vu Phuong Thy Nguyen Moon Il Kim |
| author_sort | Trung Hieu Vu |
| collection | DOAJ |
| description | Surface engineering of nanozymes has been recognized as a potent strategy to improve their catalytic activity and specificity. We synthesized polydopamine-coated Co<sub>3</sub>O<sub>4</sub> nanoparticles (PDA@Co<sub>3</sub>O<sub>4</sub> NPs) through simple dopamine-induced self-assembly and demonstrated that these NPs exhibit catalase-like activity by decomposing H<sub>2</sub>O<sub>2</sub> into oxygen and water. The activity of PDA@Co<sub>3</sub>O<sub>4</sub> NPs was approximately fourfold higher than that of Co<sub>3</sub>O<sub>4</sub> NPs without PDA, possibly due to the additional radical scavenging activity of the PDA shell. In addition, PDA@Co<sub>3</sub>O<sub>4</sub> NPs were more stable than natural catalase under a wide range of pH, temperature, and storage time conditions. Upon the addition of a sample containing sulfide ion, the activity of PDA@Co<sub>3</sub>O<sub>4</sub> NPs was significantly inhibited, possibly because of increased mass transfer limitations via the absorption of the sulfide ion on the PDA@Co<sub>3</sub>O<sub>4</sub> NP surface, along with NP aggregation which reduced their surface area. The reduced catalase-like activity was used to determine the levels of sulfide ion by measuring the increased fluorescence of the oxidized terephthalic acid, generated from the added H<sub>2</sub>O<sub>2</sub>. Using this strategy, the target sulfide ion was sensitively determined to a lower limit of 4.3 µM and dynamic linear range of up to 200 µM. The fluorescence-based sulfide ion assay based on PDA@Co<sub>3</sub>O<sub>4</sub> NPs was highly precise when applied to real tap water samples, validating its potential for conveniently monitoring toxic elements in the environment. |
| first_indexed | 2024-03-09T18:27:24Z |
| format | Article |
| id | doaj.art-6ff795ccb11c48c59a1146d106338681 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T18:27:24Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-6ff795ccb11c48c59a1146d1063386812023-11-24T07:49:04ZengMDPI AGBiosensors2079-63742022-11-011211104710.3390/bios12111047Polydopamine-Coated Co<sub>3</sub>O<sub>4</sub> Nanoparticles as an Efficient Catalase Mimic for Fluorescent Detection of Sulfide IonTrung Hieu Vu0Phuong Thy Nguyen1Moon Il Kim2Department of BioNano Technology, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam 13120, Republic of KoreaDepartment of BioNano Technology, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam 13120, Republic of KoreaDepartment of BioNano Technology, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam 13120, Republic of KoreaSurface engineering of nanozymes has been recognized as a potent strategy to improve their catalytic activity and specificity. We synthesized polydopamine-coated Co<sub>3</sub>O<sub>4</sub> nanoparticles (PDA@Co<sub>3</sub>O<sub>4</sub> NPs) through simple dopamine-induced self-assembly and demonstrated that these NPs exhibit catalase-like activity by decomposing H<sub>2</sub>O<sub>2</sub> into oxygen and water. The activity of PDA@Co<sub>3</sub>O<sub>4</sub> NPs was approximately fourfold higher than that of Co<sub>3</sub>O<sub>4</sub> NPs without PDA, possibly due to the additional radical scavenging activity of the PDA shell. In addition, PDA@Co<sub>3</sub>O<sub>4</sub> NPs were more stable than natural catalase under a wide range of pH, temperature, and storage time conditions. Upon the addition of a sample containing sulfide ion, the activity of PDA@Co<sub>3</sub>O<sub>4</sub> NPs was significantly inhibited, possibly because of increased mass transfer limitations via the absorption of the sulfide ion on the PDA@Co<sub>3</sub>O<sub>4</sub> NP surface, along with NP aggregation which reduced their surface area. The reduced catalase-like activity was used to determine the levels of sulfide ion by measuring the increased fluorescence of the oxidized terephthalic acid, generated from the added H<sub>2</sub>O<sub>2</sub>. Using this strategy, the target sulfide ion was sensitively determined to a lower limit of 4.3 µM and dynamic linear range of up to 200 µM. The fluorescence-based sulfide ion assay based on PDA@Co<sub>3</sub>O<sub>4</sub> NPs was highly precise when applied to real tap water samples, validating its potential for conveniently monitoring toxic elements in the environment.https://www.mdpi.com/2079-6374/12/11/1047polydopamine coatingcobalt oxide nanoparticlescatalase-like nanozymesulfide ion detectionfluorescent biosensors |
| spellingShingle | Trung Hieu Vu Phuong Thy Nguyen Moon Il Kim Polydopamine-Coated Co<sub>3</sub>O<sub>4</sub> Nanoparticles as an Efficient Catalase Mimic for Fluorescent Detection of Sulfide Ion Biosensors polydopamine coating cobalt oxide nanoparticles catalase-like nanozyme sulfide ion detection fluorescent biosensors |
| title | Polydopamine-Coated Co<sub>3</sub>O<sub>4</sub> Nanoparticles as an Efficient Catalase Mimic for Fluorescent Detection of Sulfide Ion |
| title_full | Polydopamine-Coated Co<sub>3</sub>O<sub>4</sub> Nanoparticles as an Efficient Catalase Mimic for Fluorescent Detection of Sulfide Ion |
| title_fullStr | Polydopamine-Coated Co<sub>3</sub>O<sub>4</sub> Nanoparticles as an Efficient Catalase Mimic for Fluorescent Detection of Sulfide Ion |
| title_full_unstemmed | Polydopamine-Coated Co<sub>3</sub>O<sub>4</sub> Nanoparticles as an Efficient Catalase Mimic for Fluorescent Detection of Sulfide Ion |
| title_short | Polydopamine-Coated Co<sub>3</sub>O<sub>4</sub> Nanoparticles as an Efficient Catalase Mimic for Fluorescent Detection of Sulfide Ion |
| title_sort | polydopamine coated co sub 3 sub o sub 4 sub nanoparticles as an efficient catalase mimic for fluorescent detection of sulfide ion |
| topic | polydopamine coating cobalt oxide nanoparticles catalase-like nanozyme sulfide ion detection fluorescent biosensors |
| url | https://www.mdpi.com/2079-6374/12/11/1047 |
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