Alterations of gut microbiota in a mouse model with partial small intestinal obstruction
IntroductionChanges in the gut microbiota of patients with partial small intestinal obstruction (PSIO) have not been widely clarified. We aimed to explore bacterial diversity in a PSIO mouse model.MethodsA PSIO mouse model was established using male C57BL/6 mice, and feces samples from the distal il...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1242650/full |
| _version_ | 1797671467217846272 |
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| author | Yong Wang Yong Wang Yong Wang Yong Wang Minzhong Zhang Lu Jiang Yiming Gong Keqiang Liu Tian Zhang |
| author_facet | Yong Wang Yong Wang Yong Wang Yong Wang Minzhong Zhang Lu Jiang Yiming Gong Keqiang Liu Tian Zhang |
| author_sort | Yong Wang |
| collection | DOAJ |
| description | IntroductionChanges in the gut microbiota of patients with partial small intestinal obstruction (PSIO) have not been widely clarified. We aimed to explore bacterial diversity in a PSIO mouse model.MethodsA PSIO mouse model was established using male C57BL/6 mice, and feces samples from the distal ileum and ileum epithelium tissues were collected. MiSeq sequencing of the 16S rRNA gene was conducted to characterize microbiota diversity and composition. RNA sequencing for differences in transcriptomic programming of the ileum tissue was performed between the PSIO and (Control) Ctrl groups.ResultsBacterial diversity in the PSIO group was significantly lower than that in the controls. Pseudomonadota was predominant in the feces of the PSIO group. Unclassified_Muribaculaceae (p = 0.008) and Akkermansia (p = 0.007) were more abundant in the Ctrl group than those in the PSIO group. Furthermore, Escherichia_Shigella (p = 0.008) was more predominant in the feces of the PSIO group. The Kyoto Encyclopedia of Genes and Genomes pathways related to metabolism were depleted in the PSIO group. Pathways associated with intestinal fibrosis, including extracellular matrix-receptor interaction, focal adhesion, phosphoinositide 3-kinase (PI3K)-Akt signaling pathway and transforming growth factor (TGF)-beta signaling pathway, which were enriched in ileum epithelial tissue in the PSIO group.ConclusionPSIO can lead to changes in the predominant intestinal bacterial groups. Depleted functional profiles of the gut microbiota were identified in the PSIO group. Functional pathways associated with intestinal fibrosis were activated by PSIO. The potential regulation by the microbiota needs to be explored in the future. |
| first_indexed | 2024-03-11T21:15:58Z |
| format | Article |
| id | doaj.art-6ffaa81cdebf4bbaa53e80113abe69e5 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-11T21:15:58Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6ffaa81cdebf4bbaa53e80113abe69e52023-09-29T04:16:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-09-011410.3389/fmicb.2023.12426501242650Alterations of gut microbiota in a mouse model with partial small intestinal obstructionYong Wang0Yong Wang1Yong Wang2Yong Wang3Minzhong Zhang4Lu Jiang5Yiming Gong6Keqiang Liu7Tian Zhang8Department of Pediatric Surgery, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangzhou, ChinaGuangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangzhou, ChinaGuangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, Guangzhou, ChinaDepartment of Pediatric Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai, ChinaDepartment of Pediatric Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Shanghai, ChinaDepartment of Pediatric Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaIntroductionChanges in the gut microbiota of patients with partial small intestinal obstruction (PSIO) have not been widely clarified. We aimed to explore bacterial diversity in a PSIO mouse model.MethodsA PSIO mouse model was established using male C57BL/6 mice, and feces samples from the distal ileum and ileum epithelium tissues were collected. MiSeq sequencing of the 16S rRNA gene was conducted to characterize microbiota diversity and composition. RNA sequencing for differences in transcriptomic programming of the ileum tissue was performed between the PSIO and (Control) Ctrl groups.ResultsBacterial diversity in the PSIO group was significantly lower than that in the controls. Pseudomonadota was predominant in the feces of the PSIO group. Unclassified_Muribaculaceae (p = 0.008) and Akkermansia (p = 0.007) were more abundant in the Ctrl group than those in the PSIO group. Furthermore, Escherichia_Shigella (p = 0.008) was more predominant in the feces of the PSIO group. The Kyoto Encyclopedia of Genes and Genomes pathways related to metabolism were depleted in the PSIO group. Pathways associated with intestinal fibrosis, including extracellular matrix-receptor interaction, focal adhesion, phosphoinositide 3-kinase (PI3K)-Akt signaling pathway and transforming growth factor (TGF)-beta signaling pathway, which were enriched in ileum epithelial tissue in the PSIO group.ConclusionPSIO can lead to changes in the predominant intestinal bacterial groups. Depleted functional profiles of the gut microbiota were identified in the PSIO group. Functional pathways associated with intestinal fibrosis were activated by PSIO. The potential regulation by the microbiota needs to be explored in the future.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1242650/fullpediatricspartial small intestinal obstructiongut microbiotadepleted diversityintestinal fibrosis |
| spellingShingle | Yong Wang Yong Wang Yong Wang Yong Wang Minzhong Zhang Lu Jiang Yiming Gong Keqiang Liu Tian Zhang Alterations of gut microbiota in a mouse model with partial small intestinal obstruction Frontiers in Microbiology pediatrics partial small intestinal obstruction gut microbiota depleted diversity intestinal fibrosis |
| title | Alterations of gut microbiota in a mouse model with partial small intestinal obstruction |
| title_full | Alterations of gut microbiota in a mouse model with partial small intestinal obstruction |
| title_fullStr | Alterations of gut microbiota in a mouse model with partial small intestinal obstruction |
| title_full_unstemmed | Alterations of gut microbiota in a mouse model with partial small intestinal obstruction |
| title_short | Alterations of gut microbiota in a mouse model with partial small intestinal obstruction |
| title_sort | alterations of gut microbiota in a mouse model with partial small intestinal obstruction |
| topic | pediatrics partial small intestinal obstruction gut microbiota depleted diversity intestinal fibrosis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1242650/full |
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