Genetically Modified Legume Plants as a Basis for Studying the Signal Regulation of Symbiosis with Nodule Bacteria
The development of legume–rhizobial symbiosis results in the formation of nitrogen-fixing root nodules. In response to rhizobial molecules, Nod factors, signal transduction is mediated by the interaction of activated receptors with downstream signaling proteins. Previously, some new regulators of th...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/10/1/9 |
| _version_ | 1797343549026467840 |
|---|---|
| author | Andrey D. Bovin Alexandra V. Dolgikh Alina M. Dymo Elizaveta S. Kantsurova Olga A. Pavlova Elena A. Dolgikh |
| author_facet | Andrey D. Bovin Alexandra V. Dolgikh Alina M. Dymo Elizaveta S. Kantsurova Olga A. Pavlova Elena A. Dolgikh |
| author_sort | Andrey D. Bovin |
| collection | DOAJ |
| description | The development of legume–rhizobial symbiosis results in the formation of nitrogen-fixing root nodules. In response to rhizobial molecules, Nod factors, signal transduction is mediated by the interaction of activated receptors with downstream signaling proteins. Previously, some new regulators of the signal pathway, such as phospholipases D, which regulate the level of phosphatidic acid (PA), as well as mitogen-activated protein kinases (MAPKs), have been identified in legumes. Since PA is an important signal messenger, we tested the hypothesis that increasing the level of proteins involved in the reversible binding of PA in plant tissues may have a positive effect on symbiosis. Our findings showed that overexpression of <i>MtSPHK1-PA</i>, encoding the PA-binding domain of sphingosine kinase 1 (SPHK1), stimulated plant growth and nodule development in legume plants. Furthermore, the influence of MAPK6 on the development of symbiosis was studied. Using genetic engineering methods, we increased MAPK6 transcriptional activity in transgenic roots, leading to an increase in the number of nodules and the biomass of pea plants. Therefore, new approaches to obtain plants with an increased efficiency of symbiosis were tested. We report here that both genes that encode signaling proteins may be used as potential targets for future modification using biotechnological approaches. |
| first_indexed | 2024-03-08T10:49:17Z |
| format | Article |
| id | doaj.art-6ffba9a9861b445ca42b4760207a9b9b |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-08T10:49:17Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-6ffba9a9861b445ca42b4760207a9b9b2024-01-26T16:49:35ZengMDPI AGHorticulturae2311-75242023-12-01101910.3390/horticulturae10010009Genetically Modified Legume Plants as a Basis for Studying the Signal Regulation of Symbiosis with Nodule BacteriaAndrey D. Bovin0Alexandra V. Dolgikh1Alina M. Dymo2Elizaveta S. Kantsurova3Olga A. Pavlova4Elena A. Dolgikh5Laboratory of Signal Regulation, All-Russia Research Institute for Agricultural Microbiology, Podbelsky Chausse 3, 196608 St. Petersburg, RussiaLaboratory of Signal Regulation, All-Russia Research Institute for Agricultural Microbiology, Podbelsky Chausse 3, 196608 St. Petersburg, RussiaLaboratory of Signal Regulation, All-Russia Research Institute for Agricultural Microbiology, Podbelsky Chausse 3, 196608 St. Petersburg, RussiaLaboratory of Signal Regulation, All-Russia Research Institute for Agricultural Microbiology, Podbelsky Chausse 3, 196608 St. Petersburg, RussiaLaboratory of Signal Regulation, All-Russia Research Institute for Agricultural Microbiology, Podbelsky Chausse 3, 196608 St. Petersburg, RussiaLaboratory of Signal Regulation, All-Russia Research Institute for Agricultural Microbiology, Podbelsky Chausse 3, 196608 St. Petersburg, RussiaThe development of legume–rhizobial symbiosis results in the formation of nitrogen-fixing root nodules. In response to rhizobial molecules, Nod factors, signal transduction is mediated by the interaction of activated receptors with downstream signaling proteins. Previously, some new regulators of the signal pathway, such as phospholipases D, which regulate the level of phosphatidic acid (PA), as well as mitogen-activated protein kinases (MAPKs), have been identified in legumes. Since PA is an important signal messenger, we tested the hypothesis that increasing the level of proteins involved in the reversible binding of PA in plant tissues may have a positive effect on symbiosis. Our findings showed that overexpression of <i>MtSPHK1-PA</i>, encoding the PA-binding domain of sphingosine kinase 1 (SPHK1), stimulated plant growth and nodule development in legume plants. Furthermore, the influence of MAPK6 on the development of symbiosis was studied. Using genetic engineering methods, we increased MAPK6 transcriptional activity in transgenic roots, leading to an increase in the number of nodules and the biomass of pea plants. Therefore, new approaches to obtain plants with an increased efficiency of symbiosis were tested. We report here that both genes that encode signaling proteins may be used as potential targets for future modification using biotechnological approaches.https://www.mdpi.com/2311-7524/10/1/9signal transductionphosphatidic acidMAP kinasegene expressiontransgenic plants<i>Medicago truncatula</i> |
| spellingShingle | Andrey D. Bovin Alexandra V. Dolgikh Alina M. Dymo Elizaveta S. Kantsurova Olga A. Pavlova Elena A. Dolgikh Genetically Modified Legume Plants as a Basis for Studying the Signal Regulation of Symbiosis with Nodule Bacteria Horticulturae signal transduction phosphatidic acid MAP kinase gene expression transgenic plants <i>Medicago truncatula</i> |
| title | Genetically Modified Legume Plants as a Basis for Studying the Signal Regulation of Symbiosis with Nodule Bacteria |
| title_full | Genetically Modified Legume Plants as a Basis for Studying the Signal Regulation of Symbiosis with Nodule Bacteria |
| title_fullStr | Genetically Modified Legume Plants as a Basis for Studying the Signal Regulation of Symbiosis with Nodule Bacteria |
| title_full_unstemmed | Genetically Modified Legume Plants as a Basis for Studying the Signal Regulation of Symbiosis with Nodule Bacteria |
| title_short | Genetically Modified Legume Plants as a Basis for Studying the Signal Regulation of Symbiosis with Nodule Bacteria |
| title_sort | genetically modified legume plants as a basis for studying the signal regulation of symbiosis with nodule bacteria |
| topic | signal transduction phosphatidic acid MAP kinase gene expression transgenic plants <i>Medicago truncatula</i> |
| url | https://www.mdpi.com/2311-7524/10/1/9 |
| work_keys_str_mv | AT andreydbovin geneticallymodifiedlegumeplantsasabasisforstudyingthesignalregulationofsymbiosiswithnodulebacteria AT alexandravdolgikh geneticallymodifiedlegumeplantsasabasisforstudyingthesignalregulationofsymbiosiswithnodulebacteria AT alinamdymo geneticallymodifiedlegumeplantsasabasisforstudyingthesignalregulationofsymbiosiswithnodulebacteria AT elizavetaskantsurova geneticallymodifiedlegumeplantsasabasisforstudyingthesignalregulationofsymbiosiswithnodulebacteria AT olgaapavlova geneticallymodifiedlegumeplantsasabasisforstudyingthesignalregulationofsymbiosiswithnodulebacteria AT elenaadolgikh geneticallymodifiedlegumeplantsasabasisforstudyingthesignalregulationofsymbiosiswithnodulebacteria |