Association of ANA seropositivity with RF, CRP, Brucellosis test in patients with SLE, a compression between immunofluorescence technique and latex agglutination
Objectives The present study aims to inspect the connection between patients with SLE and RF, CRP, Brucellosis and compression between immunofluorescence technique and latex agglutination method to detect ANA. Methods A total of 90 serum sample collected from patients with symptoms and signs of SLE...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Nab'a Al-Hayat Foundation for Medical Sciences and Health Care - Press
2017-12-01
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| Series: | Iraq Medical Journal |
| Online Access: | http://www.jocms.org/index.php/imj/article/view/284 |
| _version_ | 1811259360954810368 |
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| author | Israa Saeed Ali Mansoor AlAmeri |
| author_facet | Israa Saeed Ali Mansoor AlAmeri |
| author_sort | Israa Saeed |
| collection | DOAJ |
| description | Objectives The present study aims to inspect the connection between patients with SLE and RF, CRP, Brucellosis and compression between immunofluorescence technique and latex agglutination method to detect ANA.
Methods A total of 90 serum sample collected from patients with symptoms and signs of SLE those send to the immunity lab in Al-Hussein hospital. The immunofloriscent ANA test, SLE latex agglutination test, RF, CRP and Rose Bengal tests were done to all these samples.
Results The results show that there was significant difference between males (16%) and females (84%) patients with sign and symptom of SLE, while there was no significant difference in CRP, RF, in patients with SLE positive (50%,17%) and SLE negative patients (48.5%,17%), continuously Rose Bengal test is higher in SLE negative patients than in SLE positive patients (15.2%, 8.3%). Finally, there is significant difference in diagnosis of SLE disease between IF technique and latex agglutination test (41.6%, 100%).
Conclusions Latex agglutination test for ANA detection is reliable, specific but less sensitive test when compared to SLE IF test. There are significance differences in Rose Bengal test in SLE negative and in SLE positive patient’s, while there are non-significance difference in CRP, RF in SLE positive samples and in SLE negative samples. |
| first_indexed | 2024-04-12T18:29:05Z |
| format | Article |
| id | doaj.art-7000568866b24690b87852b7529f62d5 |
| institution | Directory Open Access Journal |
| issn | 2523-1634 2521-8492 |
| language | English |
| last_indexed | 2024-04-12T18:29:05Z |
| publishDate | 2017-12-01 |
| publisher | Nab'a Al-Hayat Foundation for Medical Sciences and Health Care - Press |
| record_format | Article |
| series | Iraq Medical Journal |
| spelling | doaj.art-7000568866b24690b87852b7529f62d52022-12-22T03:21:08ZengNab'a Al-Hayat Foundation for Medical Sciences and Health Care - PressIraq Medical Journal2523-16342521-84922017-12-0114284Association of ANA seropositivity with RF, CRP, Brucellosis test in patients with SLE, a compression between immunofluorescence technique and latex agglutinationIsraa Saeed0Ali Mansoor AlAmeri1College of Applied Medical Science, University of Kerbala, Iraq.Department of Microbiology, University of Kerbala, College of Medicine, Karbala, Iraq.Objectives The present study aims to inspect the connection between patients with SLE and RF, CRP, Brucellosis and compression between immunofluorescence technique and latex agglutination method to detect ANA. Methods A total of 90 serum sample collected from patients with symptoms and signs of SLE those send to the immunity lab in Al-Hussein hospital. The immunofloriscent ANA test, SLE latex agglutination test, RF, CRP and Rose Bengal tests were done to all these samples. Results The results show that there was significant difference between males (16%) and females (84%) patients with sign and symptom of SLE, while there was no significant difference in CRP, RF, in patients with SLE positive (50%,17%) and SLE negative patients (48.5%,17%), continuously Rose Bengal test is higher in SLE negative patients than in SLE positive patients (15.2%, 8.3%). Finally, there is significant difference in diagnosis of SLE disease between IF technique and latex agglutination test (41.6%, 100%). Conclusions Latex agglutination test for ANA detection is reliable, specific but less sensitive test when compared to SLE IF test. There are significance differences in Rose Bengal test in SLE negative and in SLE positive patient’s, while there are non-significance difference in CRP, RF in SLE positive samples and in SLE negative samples.http://www.jocms.org/index.php/imj/article/view/284 |
| spellingShingle | Israa Saeed Ali Mansoor AlAmeri Association of ANA seropositivity with RF, CRP, Brucellosis test in patients with SLE, a compression between immunofluorescence technique and latex agglutination Iraq Medical Journal |
| title | Association of ANA seropositivity with RF, CRP, Brucellosis test in patients with SLE, a compression between immunofluorescence technique and latex agglutination |
| title_full | Association of ANA seropositivity with RF, CRP, Brucellosis test in patients with SLE, a compression between immunofluorescence technique and latex agglutination |
| title_fullStr | Association of ANA seropositivity with RF, CRP, Brucellosis test in patients with SLE, a compression between immunofluorescence technique and latex agglutination |
| title_full_unstemmed | Association of ANA seropositivity with RF, CRP, Brucellosis test in patients with SLE, a compression between immunofluorescence technique and latex agglutination |
| title_short | Association of ANA seropositivity with RF, CRP, Brucellosis test in patients with SLE, a compression between immunofluorescence technique and latex agglutination |
| title_sort | association of ana seropositivity with rf crp brucellosis test in patients with sle a compression between immunofluorescence technique and latex agglutination |
| url | http://www.jocms.org/index.php/imj/article/view/284 |
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