Regulation of Aquaporin Prip Expression and Its Physiological Function in <i>Rhyzopertha dominica</i> (Coleoptera: Bostrichidae)
<i>Rhyzopertha dominica</i> Prip (<i>RdPrip</i>) cDNA was cloned (GenBank accession no. OK318454), and the encoded 276-amino-acid protein indicated the typical aquaporin structure, including six transmembrane regions and two NPA motifs. The developmental and tissue profiles o...
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MDPI AG
2023-01-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/1/70 |
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| author | Lan-Pin Tan Mei-Er Chen |
| author_facet | Lan-Pin Tan Mei-Er Chen |
| author_sort | Lan-Pin Tan |
| collection | DOAJ |
| description | <i>Rhyzopertha dominica</i> Prip (<i>RdPrip</i>) cDNA was cloned (GenBank accession no. OK318454), and the encoded 276-amino-acid protein indicated the typical aquaporin structure, including six transmembrane regions and two NPA motifs. The developmental and tissue profiles of <i>RdPrip</i> transcription were determined. <i>RdPrip</i> was highly transcribed in female adults, followed by larvae, pupae, and male adults. The transcriptional expression levels of <i>RdPrip</i> were significantly high in the ovary and hindgut (including cryptonephridial systems) compared with the foregut, testis, midgut, and Malpighian tubules. Knockdown of <i>RdPrip</i> in female adults did not decrease fecundity, but significantly decreased the hatching rate of eggs laid by the females. The results suggest that <i>RdPrip</i> functions in embryonic development, not in egg formation. In addition, the transcriptional expression level of <i>RdPrip</i> was lower in the spinosad-resistant strain than in the susceptible one, and the resistant strain produced fewer progeny than the susceptible strain did. These studies support the functional role of <i>RdPrip</i> in female reproduction. The absence of significant mortality reduction in the <i>R. dominica</i> exposed to spinosad after <i>RdPrip</i> RNAi suggests that other aquaporins that were not knocked down may exist for the excretion of metabolized pesticides. |
| first_indexed | 2024-03-09T12:13:52Z |
| format | Article |
| id | doaj.art-70012c0d514b48e89b329d52dd32ba62 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T12:13:52Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-70012c0d514b48e89b329d52dd32ba622023-11-30T22:48:34ZengMDPI AGInsects2075-44502023-01-011417010.3390/insects14010070Regulation of Aquaporin Prip Expression and Its Physiological Function in <i>Rhyzopertha dominica</i> (Coleoptera: Bostrichidae)Lan-Pin Tan0Mei-Er Chen1Department of Entomology, National Chung-Hsing University, Taichung City 40227, TaiwanDepartment of Entomology, National Chung-Hsing University, Taichung City 40227, Taiwan<i>Rhyzopertha dominica</i> Prip (<i>RdPrip</i>) cDNA was cloned (GenBank accession no. OK318454), and the encoded 276-amino-acid protein indicated the typical aquaporin structure, including six transmembrane regions and two NPA motifs. The developmental and tissue profiles of <i>RdPrip</i> transcription were determined. <i>RdPrip</i> was highly transcribed in female adults, followed by larvae, pupae, and male adults. The transcriptional expression levels of <i>RdPrip</i> were significantly high in the ovary and hindgut (including cryptonephridial systems) compared with the foregut, testis, midgut, and Malpighian tubules. Knockdown of <i>RdPrip</i> in female adults did not decrease fecundity, but significantly decreased the hatching rate of eggs laid by the females. The results suggest that <i>RdPrip</i> functions in embryonic development, not in egg formation. In addition, the transcriptional expression level of <i>RdPrip</i> was lower in the spinosad-resistant strain than in the susceptible one, and the resistant strain produced fewer progeny than the susceptible strain did. These studies support the functional role of <i>RdPrip</i> in female reproduction. The absence of significant mortality reduction in the <i>R. dominica</i> exposed to spinosad after <i>RdPrip</i> RNAi suggests that other aquaporins that were not knocked down may exist for the excretion of metabolized pesticides.https://www.mdpi.com/2075-4450/14/1/70<i>Rhyzopertha dominica</i>aquaporin priptranscriptional expressionovaryRNAi |
| spellingShingle | Lan-Pin Tan Mei-Er Chen Regulation of Aquaporin Prip Expression and Its Physiological Function in <i>Rhyzopertha dominica</i> (Coleoptera: Bostrichidae) Insects <i>Rhyzopertha dominica</i> aquaporin prip transcriptional expression ovary RNAi |
| title | Regulation of Aquaporin Prip Expression and Its Physiological Function in <i>Rhyzopertha dominica</i> (Coleoptera: Bostrichidae) |
| title_full | Regulation of Aquaporin Prip Expression and Its Physiological Function in <i>Rhyzopertha dominica</i> (Coleoptera: Bostrichidae) |
| title_fullStr | Regulation of Aquaporin Prip Expression and Its Physiological Function in <i>Rhyzopertha dominica</i> (Coleoptera: Bostrichidae) |
| title_full_unstemmed | Regulation of Aquaporin Prip Expression and Its Physiological Function in <i>Rhyzopertha dominica</i> (Coleoptera: Bostrichidae) |
| title_short | Regulation of Aquaporin Prip Expression and Its Physiological Function in <i>Rhyzopertha dominica</i> (Coleoptera: Bostrichidae) |
| title_sort | regulation of aquaporin prip expression and its physiological function in i rhyzopertha dominica i coleoptera bostrichidae |
| topic | <i>Rhyzopertha dominica</i> aquaporin prip transcriptional expression ovary RNAi |
| url | https://www.mdpi.com/2075-4450/14/1/70 |
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