Changes in mast cell infiltration: a possible mechanism in detrusor overactivity induced by visceral hypersensitivity
ABSTRACT Objective: To establish the detrusor overactivity (DO) model induced by visceral hypersensitivity (VH) and investigate the relationship between mast cell (MC) infiltration and DO. Materials and Methods: Sixty rats are divided into 4 groups randomly: Group 1:Baseline group; Group 2: DO gro...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Urologia
2016-04-01
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| Series: | International Brazilian Journal of Urology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382016000200373&lng=en&tlng=en |
| _version_ | 1819264010873208832 |
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| author | Nian-zhao Zhang Lin Ma Chen Jun Yan-xia Guo Hui-qing Yuan |
| author_facet | Nian-zhao Zhang Lin Ma Chen Jun Yan-xia Guo Hui-qing Yuan |
| author_sort | Nian-zhao Zhang |
| collection | DOAJ |
| description | ABSTRACT Objective: To establish the detrusor overactivity (DO) model induced by visceral hypersensitivity (VH) and investigate the relationship between mast cell (MC) infiltration and DO. Materials and Methods: Sixty rats are divided into 4 groups randomly: Group 1:Baseline group; Group 2: DO group; Group 3: CON group; Group 4: VH group. The colorectal distension (CRD) and abdominal withdral reflex (AWR) scores are performed to evaluate VH. The cystometric investigation and histological test of MC infiltration are assessed. Results: The threshold pressure of CRD in the VH group is significantly lower than that in the CON group (P<0.001). At the distension pressure ≥20 mmHg, the AWR scores of the VH group are significantly higher than those of the CON group (10 mmHg: P=0.33; 20 mmHg: P=0.028; 40 mmHg: P<0.001; 60 mmHg: P<0.001; 80 mmHg: P<0.001). DO model is successfully established in the VH group (DO rate=100%). Compared with the CON group, the numbers of MC infiltration are significantly increased in the VH group, including submucosa of bladder (P<0.001), mucosa lamina propria/mesentery of small intestine (P<0.001), and mucosa lamina propria/mesentery of large intestine (P<0.001). Furthermore, more MC activation as well as degranulation are observed in the VH group. Conclusions: It is indicated that DO model can be established in the VH rats. The MC infiltration may play an important role in DO induced by VH, and may be helpful to understand the mechanisms of DO in VH patients. |
| first_indexed | 2024-12-23T20:22:41Z |
| format | Article |
| id | doaj.art-7001611ac74b4f6695522bf86e037d0a |
| institution | Directory Open Access Journal |
| issn | 1677-6119 |
| language | English |
| last_indexed | 2024-12-23T20:22:41Z |
| publishDate | 2016-04-01 |
| publisher | Sociedade Brasileira de Urologia |
| record_format | Article |
| series | International Brazilian Journal of Urology |
| spelling | doaj.art-7001611ac74b4f6695522bf86e037d0a2022-12-21T17:32:30ZengSociedade Brasileira de UrologiaInternational Brazilian Journal of Urology1677-61192016-04-0142237338210.1590/S1677-5538.IBJU.2015.0025S1677-55382016000200373Changes in mast cell infiltration: a possible mechanism in detrusor overactivity induced by visceral hypersensitivityNian-zhao ZhangLin MaChen JunYan-xia GuoHui-qing YuanABSTRACT Objective: To establish the detrusor overactivity (DO) model induced by visceral hypersensitivity (VH) and investigate the relationship between mast cell (MC) infiltration and DO. Materials and Methods: Sixty rats are divided into 4 groups randomly: Group 1:Baseline group; Group 2: DO group; Group 3: CON group; Group 4: VH group. The colorectal distension (CRD) and abdominal withdral reflex (AWR) scores are performed to evaluate VH. The cystometric investigation and histological test of MC infiltration are assessed. Results: The threshold pressure of CRD in the VH group is significantly lower than that in the CON group (P<0.001). At the distension pressure ≥20 mmHg, the AWR scores of the VH group are significantly higher than those of the CON group (10 mmHg: P=0.33; 20 mmHg: P=0.028; 40 mmHg: P<0.001; 60 mmHg: P<0.001; 80 mmHg: P<0.001). DO model is successfully established in the VH group (DO rate=100%). Compared with the CON group, the numbers of MC infiltration are significantly increased in the VH group, including submucosa of bladder (P<0.001), mucosa lamina propria/mesentery of small intestine (P<0.001), and mucosa lamina propria/mesentery of large intestine (P<0.001). Furthermore, more MC activation as well as degranulation are observed in the VH group. Conclusions: It is indicated that DO model can be established in the VH rats. The MC infiltration may play an important role in DO induced by VH, and may be helpful to understand the mechanisms of DO in VH patients.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382016000200373&lng=en&tlng=enUrinary BladderOveractiveHypersensitivityMast-Cell Sarcoma |
| spellingShingle | Nian-zhao Zhang Lin Ma Chen Jun Yan-xia Guo Hui-qing Yuan Changes in mast cell infiltration: a possible mechanism in detrusor overactivity induced by visceral hypersensitivity International Brazilian Journal of Urology Urinary Bladder Overactive Hypersensitivity Mast-Cell Sarcoma |
| title | Changes in mast cell infiltration: a possible mechanism in detrusor overactivity induced by visceral hypersensitivity |
| title_full | Changes in mast cell infiltration: a possible mechanism in detrusor overactivity induced by visceral hypersensitivity |
| title_fullStr | Changes in mast cell infiltration: a possible mechanism in detrusor overactivity induced by visceral hypersensitivity |
| title_full_unstemmed | Changes in mast cell infiltration: a possible mechanism in detrusor overactivity induced by visceral hypersensitivity |
| title_short | Changes in mast cell infiltration: a possible mechanism in detrusor overactivity induced by visceral hypersensitivity |
| title_sort | changes in mast cell infiltration a possible mechanism in detrusor overactivity induced by visceral hypersensitivity |
| topic | Urinary Bladder Overactive Hypersensitivity Mast-Cell Sarcoma |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382016000200373&lng=en&tlng=en |
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