Evaluation of genetic variability in Algerian clover (Trifolium L.) based on morphological and isozyme markers
Genetic variation within and among fifteen Trifolium species represented by 157 accessions was assessed using morphological and isozyme markers. Most of morphometric characters contributed to the discrimination of the species. No significant relationship between the environment of the collection sit...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2015-06-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-201502-0002_evaluation-of-genetic-variability-in-algerian-clover-trifolium-l-based-on-morphological-and-isozyme-markers.php |
| _version_ | 1797899408904290304 |
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| author | Imane MEDOUKALI Inès BELLIL Douadi KHELIFI |
| author_facet | Imane MEDOUKALI Inès BELLIL Douadi KHELIFI |
| author_sort | Imane MEDOUKALI |
| collection | DOAJ |
| description | Genetic variation within and among fifteen Trifolium species represented by 157 accessions was assessed using morphological and isozyme markers. Most of morphometric characters contributed to the discrimination of the species. No significant relationship between the environment of the collection site and morphological features was detected. The two isozyme systems analysed, esterase (EST) and glutamate oxaloacetate transaminase (GOT), proved polymorphic. Phenotype diversity of isozyme markers ranged from 0.07 to 0.61 with an average of 0.31 based on the polymorphic information content. The pairwise Jaccard's similarity coefficient ranged between 0.10 and 0.60, indicating that the collection represents genetically diverse species. A considerable number of species-specific zymograms were detected which can be used for the species identification. The clustering pattern of isozyme markers was incongruent with the groupings based on quantitative traits. The rich isozyme variability present among the Algerian clover species indicates that they can provide good gene resources for breeding. |
| first_indexed | 2024-04-10T08:29:26Z |
| format | Article |
| id | doaj.art-7007f4b59bae4b41be889432f0a1ec4e |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:29:26Z |
| publishDate | 2015-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-7007f4b59bae4b41be889432f0a1ec4e2023-02-23T03:30:29ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252015-06-01512506110.17221/10/2015-CJGPBcjg-201502-0002Evaluation of genetic variability in Algerian clover (Trifolium L.) based on morphological and isozyme markersImane MEDOUKALI0Inès BELLIL1Douadi KHELIFI2Laboratoire de Génétique Biochimie et Biotechnologies Végétales, Faculté des Sciences de la Nature et de la Vie, Université Constantine, Constantine, AlgérieLaboratoire de Génétique Biochimie et Biotechnologies Végétales, Faculté des Sciences de la Nature et de la Vie, Université Constantine, Constantine, AlgérieLaboratoire de Génétique Biochimie et Biotechnologies Végétales, Faculté des Sciences de la Nature et de la Vie, Université Constantine, Constantine, AlgérieGenetic variation within and among fifteen Trifolium species represented by 157 accessions was assessed using morphological and isozyme markers. Most of morphometric characters contributed to the discrimination of the species. No significant relationship between the environment of the collection site and morphological features was detected. The two isozyme systems analysed, esterase (EST) and glutamate oxaloacetate transaminase (GOT), proved polymorphic. Phenotype diversity of isozyme markers ranged from 0.07 to 0.61 with an average of 0.31 based on the polymorphic information content. The pairwise Jaccard's similarity coefficient ranged between 0.10 and 0.60, indicating that the collection represents genetically diverse species. A considerable number of species-specific zymograms were detected which can be used for the species identification. The clustering pattern of isozyme markers was incongruent with the groupings based on quantitative traits. The rich isozyme variability present among the Algerian clover species indicates that they can provide good gene resources for breeding.https://cjgpb.agriculturejournals.cz/artkey/cjg-201502-0002_evaluation-of-genetic-variability-in-algerian-clover-trifolium-l-based-on-morphological-and-isozyme-markers.phpcloverisozymesmorphologypagepolymorphism |
| spellingShingle | Imane MEDOUKALI Inès BELLIL Douadi KHELIFI Evaluation of genetic variability in Algerian clover (Trifolium L.) based on morphological and isozyme markers Czech Journal of Genetics and Plant Breeding clover isozymes morphology page polymorphism |
| title | Evaluation of genetic variability in Algerian clover (Trifolium L.) based on morphological and isozyme markers |
| title_full | Evaluation of genetic variability in Algerian clover (Trifolium L.) based on morphological and isozyme markers |
| title_fullStr | Evaluation of genetic variability in Algerian clover (Trifolium L.) based on morphological and isozyme markers |
| title_full_unstemmed | Evaluation of genetic variability in Algerian clover (Trifolium L.) based on morphological and isozyme markers |
| title_short | Evaluation of genetic variability in Algerian clover (Trifolium L.) based on morphological and isozyme markers |
| title_sort | evaluation of genetic variability in algerian clover trifolium l based on morphological and isozyme markers |
| topic | clover isozymes morphology page polymorphism |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-201502-0002_evaluation-of-genetic-variability-in-algerian-clover-trifolium-l-based-on-morphological-and-isozyme-markers.php |
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