New Biocomposite Electrospun Fiber/Alginate Hydrogel for Probiotic Bacteria Immobilization
Biotechnological use of probiotic microorganisms involves providing them with appropriate conditions for growth, but also protection against environmental changes caused by an exchange of the medium, isolation of metabolites, etc. Therefore, the research on effective immobilization of probiotic micr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/14/14/3861 |
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| author | Adam Grzywaczyk Agata Zdarta Katarzyna Jankowska Andrzej Biadasz Jakub Zdarta Teofil Jesionowski Ewa Kaczorek Wojciech Smułek |
| author_facet | Adam Grzywaczyk Agata Zdarta Katarzyna Jankowska Andrzej Biadasz Jakub Zdarta Teofil Jesionowski Ewa Kaczorek Wojciech Smułek |
| author_sort | Adam Grzywaczyk |
| collection | DOAJ |
| description | Biotechnological use of probiotic microorganisms involves providing them with appropriate conditions for growth, but also protection against environmental changes caused by an exchange of the medium, isolation of metabolites, etc. Therefore, the research on effective immobilization of probiotic microorganisms should be focused in this direction. The present study aimed to evaluate the effectiveness of an innovative hybrid immobilization system based on electrospun nanofibers and alginate hydrogel. The analyses carried out included the study of properties of the initial components, the evaluation of the degree and durability of cell immobilization in the final material, and their survival under stress conditions. Effective binding of microorganisms to the hydrogel and nanofibers was confirmed, and the collected results proved that the proposed biocomposite is an efficient method of cell protection. In addition, it was shown that immobilization on electrospun nanofibers leads to the preservation of the highest cell activity and the least cell growth restriction as compared to free or lyophilized cells only. The completed research opens new perspectives for the effective immobilization of microorganisms of significant economic importance. |
| first_indexed | 2024-03-10T09:33:28Z |
| format | Article |
| id | doaj.art-70082fc03dcc4672be18faebf122aeb4 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T09:33:28Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-70082fc03dcc4672be18faebf122aeb42023-11-22T04:15:56ZengMDPI AGMaterials1996-19442021-07-011414386110.3390/ma14143861New Biocomposite Electrospun Fiber/Alginate Hydrogel for Probiotic Bacteria ImmobilizationAdam Grzywaczyk0Agata Zdarta1Katarzyna Jankowska2Andrzej Biadasz3Jakub Zdarta4Teofil Jesionowski5Ewa Kaczorek6Wojciech Smułek7Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, PolandInstitute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, PolandInstitute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, PolandInstitute of Physics, Faculty of Materials Engineering and Technical Physics, Poznan University of Technology, Piotrowo 3, 60-965 Poznan, PolandInstitute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, PolandInstitute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, PolandInstitute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, PolandInstitute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, PolandBiotechnological use of probiotic microorganisms involves providing them with appropriate conditions for growth, but also protection against environmental changes caused by an exchange of the medium, isolation of metabolites, etc. Therefore, the research on effective immobilization of probiotic microorganisms should be focused in this direction. The present study aimed to evaluate the effectiveness of an innovative hybrid immobilization system based on electrospun nanofibers and alginate hydrogel. The analyses carried out included the study of properties of the initial components, the evaluation of the degree and durability of cell immobilization in the final material, and their survival under stress conditions. Effective binding of microorganisms to the hydrogel and nanofibers was confirmed, and the collected results proved that the proposed biocomposite is an efficient method of cell protection. In addition, it was shown that immobilization on electrospun nanofibers leads to the preservation of the highest cell activity and the least cell growth restriction as compared to free or lyophilized cells only. The completed research opens new perspectives for the effective immobilization of microorganisms of significant economic importance.https://www.mdpi.com/1996-1944/14/14/3861biocompositecontact angleelectrospun nanofibersimmobilization<i>Lactobacillus</i>alginate hydrogel |
| spellingShingle | Adam Grzywaczyk Agata Zdarta Katarzyna Jankowska Andrzej Biadasz Jakub Zdarta Teofil Jesionowski Ewa Kaczorek Wojciech Smułek New Biocomposite Electrospun Fiber/Alginate Hydrogel for Probiotic Bacteria Immobilization Materials biocomposite contact angle electrospun nanofibers immobilization <i>Lactobacillus</i> alginate hydrogel |
| title | New Biocomposite Electrospun Fiber/Alginate Hydrogel for Probiotic Bacteria Immobilization |
| title_full | New Biocomposite Electrospun Fiber/Alginate Hydrogel for Probiotic Bacteria Immobilization |
| title_fullStr | New Biocomposite Electrospun Fiber/Alginate Hydrogel for Probiotic Bacteria Immobilization |
| title_full_unstemmed | New Biocomposite Electrospun Fiber/Alginate Hydrogel for Probiotic Bacteria Immobilization |
| title_short | New Biocomposite Electrospun Fiber/Alginate Hydrogel for Probiotic Bacteria Immobilization |
| title_sort | new biocomposite electrospun fiber alginate hydrogel for probiotic bacteria immobilization |
| topic | biocomposite contact angle electrospun nanofibers immobilization <i>Lactobacillus</i> alginate hydrogel |
| url | https://www.mdpi.com/1996-1944/14/14/3861 |
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