Characterization of Lactic Acid Bacteria Strains Isolated from Algerian Honeybee and Honey and Exploration of Their Potential Probiotic and Functional Features for Human Use
Using culture enrichment methods, 100 strains of bacilli of lactic acid bacteria (LAB) were isolated from honeybee <i>Apis mellifera intermissa</i> and fresh honey, collected from apiaries located in the north-east of Algeria. Amongst all of the isolated LAB, 19 selected strains were clo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/12/2312 |
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| author | Meriem Meradji Nadia Bachtarzi Diego Mora Karima Kharroub |
| author_facet | Meriem Meradji Nadia Bachtarzi Diego Mora Karima Kharroub |
| author_sort | Meriem Meradji |
| collection | DOAJ |
| description | Using culture enrichment methods, 100 strains of bacilli of lactic acid bacteria (LAB) were isolated from honeybee <i>Apis mellifera intermissa</i> and fresh honey, collected from apiaries located in the north-east of Algeria. Amongst all of the isolated LAB, 19 selected strains were closely affiliated to four species—<i>Fructobacillus fructosus</i> (10), <i>Apilactobacillus kunkeei</i> (5), <i>Lactobacillus kimbladii</i> and/or <i>Lactobacillus kullabergensis</i> (4)—using phylogenetic and phenotypic approaches. The in vitro probiotic characteristics (simulated gastrointestinal fluids tolerance, autoaggregation and hydrophobicity abilities, antimicrobial activity and cholesterol reduction) and safety properties (hemolytic activity, antibiotic resistance and absence of biogenic amines) were evaluated. The results indicated that some strains showed promising potential probiotic properties. In addition, neither hemolytic activity nor biogenic amines were produced. The carbohydrate fermentation test (API 50 CHL) revealed that the strains could efficiently use a broad range of carbohydrates; additionally, four strains belonging to <i>Apilactobacillus kunkeei</i> and <i>Fructobacillus fructosus</i> were found to be exopolysaccharides (EPS) producers. This study demonstrates the honeybee <i>Apis mellifera intermissa</i> and one of her products as a reservoir for novel LAB with potential probiotic features, suggesting suitability for promoting host health. |
| first_indexed | 2024-03-11T02:28:46Z |
| format | Article |
| id | doaj.art-700a5efa17c342a5a426aac43b4c5e6d |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T02:28:46Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-700a5efa17c342a5a426aac43b4c5e6d2023-11-18T10:23:58ZengMDPI AGFoods2304-81582023-06-011212231210.3390/foods12122312Characterization of Lactic Acid Bacteria Strains Isolated from Algerian Honeybee and Honey and Exploration of Their Potential Probiotic and Functional Features for Human UseMeriem Meradji0Nadia Bachtarzi1Diego Mora2Karima Kharroub3Laboratoire de Recherche Biotechnologie et Qualité des Aliments (BIOQUAL), Institut de la Nutrition, de l’Alimentation et des Technologies Agro-Alimentaires (INATAA), Université Frères Mentouri Constantine 1 (UFMC1), Route de Ain El Bey, Constantine 25000, AlgeriaLaboratoire de Recherche Biotechnologie et Qualité des Aliments (BIOQUAL), Institut de la Nutrition, de l’Alimentation et des Technologies Agro-Alimentaires (INATAA), Université Frères Mentouri Constantine 1 (UFMC1), Route de Ain El Bey, Constantine 25000, AlgeriaDepartment of Food Environmental and Nutritional Sciences (DeFENS), University of Milan, 20122 Milan, ItalyLaboratoire de Recherche Biotechnologie et Qualité des Aliments (BIOQUAL), Institut de la Nutrition, de l’Alimentation et des Technologies Agro-Alimentaires (INATAA), Université Frères Mentouri Constantine 1 (UFMC1), Route de Ain El Bey, Constantine 25000, AlgeriaUsing culture enrichment methods, 100 strains of bacilli of lactic acid bacteria (LAB) were isolated from honeybee <i>Apis mellifera intermissa</i> and fresh honey, collected from apiaries located in the north-east of Algeria. Amongst all of the isolated LAB, 19 selected strains were closely affiliated to four species—<i>Fructobacillus fructosus</i> (10), <i>Apilactobacillus kunkeei</i> (5), <i>Lactobacillus kimbladii</i> and/or <i>Lactobacillus kullabergensis</i> (4)—using phylogenetic and phenotypic approaches. The in vitro probiotic characteristics (simulated gastrointestinal fluids tolerance, autoaggregation and hydrophobicity abilities, antimicrobial activity and cholesterol reduction) and safety properties (hemolytic activity, antibiotic resistance and absence of biogenic amines) were evaluated. The results indicated that some strains showed promising potential probiotic properties. In addition, neither hemolytic activity nor biogenic amines were produced. The carbohydrate fermentation test (API 50 CHL) revealed that the strains could efficiently use a broad range of carbohydrates; additionally, four strains belonging to <i>Apilactobacillus kunkeei</i> and <i>Fructobacillus fructosus</i> were found to be exopolysaccharides (EPS) producers. This study demonstrates the honeybee <i>Apis mellifera intermissa</i> and one of her products as a reservoir for novel LAB with potential probiotic features, suggesting suitability for promoting host health.https://www.mdpi.com/2304-8158/12/12/2312<i>Apis mellifera</i> intermissahoney<i>Apilactobacillus</i> sp.<i>Fructobacillus</i> sp.<i>Lactobacillus</i> sp.probiotic |
| spellingShingle | Meriem Meradji Nadia Bachtarzi Diego Mora Karima Kharroub Characterization of Lactic Acid Bacteria Strains Isolated from Algerian Honeybee and Honey and Exploration of Their Potential Probiotic and Functional Features for Human Use Foods <i>Apis mellifera</i> intermissa honey <i>Apilactobacillus</i> sp. <i>Fructobacillus</i> sp. <i>Lactobacillus</i> sp. probiotic |
| title | Characterization of Lactic Acid Bacteria Strains Isolated from Algerian Honeybee and Honey and Exploration of Their Potential Probiotic and Functional Features for Human Use |
| title_full | Characterization of Lactic Acid Bacteria Strains Isolated from Algerian Honeybee and Honey and Exploration of Their Potential Probiotic and Functional Features for Human Use |
| title_fullStr | Characterization of Lactic Acid Bacteria Strains Isolated from Algerian Honeybee and Honey and Exploration of Their Potential Probiotic and Functional Features for Human Use |
| title_full_unstemmed | Characterization of Lactic Acid Bacteria Strains Isolated from Algerian Honeybee and Honey and Exploration of Their Potential Probiotic and Functional Features for Human Use |
| title_short | Characterization of Lactic Acid Bacteria Strains Isolated from Algerian Honeybee and Honey and Exploration of Their Potential Probiotic and Functional Features for Human Use |
| title_sort | characterization of lactic acid bacteria strains isolated from algerian honeybee and honey and exploration of their potential probiotic and functional features for human use |
| topic | <i>Apis mellifera</i> intermissa honey <i>Apilactobacillus</i> sp. <i>Fructobacillus</i> sp. <i>Lactobacillus</i> sp. probiotic |
| url | https://www.mdpi.com/2304-8158/12/12/2312 |
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