Characteristics of dual carbapenemase-producing Klebsiella pneumoniae strains from an outbreak in Venezuela: a retrospective study
Objective. To characterize carbapenemase-producing Klebsiella pneumoniae isolated from patients treated at a hospital in Cumaná, Sucre, Venezuela. Methods. This was a retrospective study conducted at the general hospital in Cumaná where 58 K. pneumoniae strains were analyzed for resistance to antimi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Pan American Health Organization
2020-09-01
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| Series: | Revista Panamericana de Salud Pública |
| Subjects: | |
| Online Access: | https://iris.paho.org/handle/10665.2/52322 |
| _version_ | 1818274409458171904 |
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| author | Dianny Martínez Luisa Caña Hectorina Rodulfo José García Diorelis González Lucy Rodríguez Marcos De Donato |
| author_facet | Dianny Martínez Luisa Caña Hectorina Rodulfo José García Diorelis González Lucy Rodríguez Marcos De Donato |
| author_sort | Dianny Martínez |
| collection | DOAJ |
| description | Objective. To characterize carbapenemase-producing Klebsiella pneumoniae isolated from patients treated at a hospital in Cumaná, Sucre, Venezuela.
Methods. This was a retrospective study conducted at the general hospital in Cumaná where 58 K. pneumoniae strains were analyzed for resistance to antimicrobials, specifically carbapenems, in January – June 2015. Production of metallo-β-lactamases and serine carbapenemases was determined by the double-disc synergy test, using EDTA-sodium mercaptoacetic acid and 3-aminophenyl boronic acid discs, respectively. Multiplex-PCR was used to detect genes coding for carbapenemases. Molecular typing using ERIC-PCR determined the presence of clones.
Results. Four strains of K. pneumoniae resistant to carbapenems were identified. Phenotypic methods for detection of metallo-β-lactamases and serine carbapenemases were positive, and PCR demonstrated the co-presence of blaNDM and blaKPC genes in all four strains. ERIC-PCR identified two clones circulating in the hospital.
Conclusions. Infection control strategies are needed at the central hospital in Cumaná and its surrounding areas to prevent the spread of these pathogens, especially given the high levels of migration from Venezuela to other countries in South America. |
| first_indexed | 2024-12-12T22:13:24Z |
| format | Article |
| id | doaj.art-700da5ceb79e4b97a85a78dbc46516f1 |
| institution | Directory Open Access Journal |
| issn | 1020-4989 1680-5348 |
| language | English |
| last_indexed | 2024-12-12T22:13:24Z |
| publishDate | 2020-09-01 |
| publisher | Pan American Health Organization |
| record_format | Article |
| series | Revista Panamericana de Salud Pública |
| spelling | doaj.art-700da5ceb79e4b97a85a78dbc46516f12022-12-22T00:10:10ZengPan American Health OrganizationRevista Panamericana de Salud Pública1020-49891680-53482020-09-0144501710.26633/RPSP.2020.50rpspCharacteristics of dual carbapenemase-producing Klebsiella pneumoniae strains from an outbreak in Venezuela: a retrospective studyDianny Martínez0Luisa Caña1Hectorina Rodulfo2José García3Diorelis González4Lucy Rodríguez5Marcos De Donato6Clinical Bacteriology Laboratory, Antonio Patricio de Alcalá University Hospital, Cumaná, Venezuela.Clinical Bacteriology Laboratory, Antonio Patricio de Alcalá University Hospital, Cumaná, Venezuela.Tecnológico de Monterrey, Escuela de Ingeniería y Ciencias, Querétaro, Mexico.Clinical Bacteriology Laboratory, Antonio Patricio de Alcalá University Hospital, Cumaná, Venezuela.Clinical Bacteriology Laboratory, Antonio Patricio de Alcalá University Hospital, Cumaná, Venezuela.Clinical Bacteriology Laboratory, Antonio Patricio de Alcalá University Hospital, Cumaná, Venezuela.Tecnológico de Monterrey, Escuela de Ingeniería y Ciencias, Querétaro, Mexico.Objective. To characterize carbapenemase-producing Klebsiella pneumoniae isolated from patients treated at a hospital in Cumaná, Sucre, Venezuela. Methods. This was a retrospective study conducted at the general hospital in Cumaná where 58 K. pneumoniae strains were analyzed for resistance to antimicrobials, specifically carbapenems, in January – June 2015. Production of metallo-β-lactamases and serine carbapenemases was determined by the double-disc synergy test, using EDTA-sodium mercaptoacetic acid and 3-aminophenyl boronic acid discs, respectively. Multiplex-PCR was used to detect genes coding for carbapenemases. Molecular typing using ERIC-PCR determined the presence of clones. Results. Four strains of K. pneumoniae resistant to carbapenems were identified. Phenotypic methods for detection of metallo-β-lactamases and serine carbapenemases were positive, and PCR demonstrated the co-presence of blaNDM and blaKPC genes in all four strains. ERIC-PCR identified two clones circulating in the hospital. Conclusions. Infection control strategies are needed at the central hospital in Cumaná and its surrounding areas to prevent the spread of these pathogens, especially given the high levels of migration from Venezuela to other countries in South America.https://iris.paho.org/handle/10665.2/52322klebsiella pneumoniaecarbapenem-resistant enterobacteriaceaemolecular typingvenezuela |
| spellingShingle | Dianny Martínez Luisa Caña Hectorina Rodulfo José García Diorelis González Lucy Rodríguez Marcos De Donato Characteristics of dual carbapenemase-producing Klebsiella pneumoniae strains from an outbreak in Venezuela: a retrospective study Revista Panamericana de Salud Pública klebsiella pneumoniae carbapenem-resistant enterobacteriaceae molecular typing venezuela |
| title | Characteristics of dual carbapenemase-producing Klebsiella pneumoniae strains from an outbreak in Venezuela: a retrospective study |
| title_full | Characteristics of dual carbapenemase-producing Klebsiella pneumoniae strains from an outbreak in Venezuela: a retrospective study |
| title_fullStr | Characteristics of dual carbapenemase-producing Klebsiella pneumoniae strains from an outbreak in Venezuela: a retrospective study |
| title_full_unstemmed | Characteristics of dual carbapenemase-producing Klebsiella pneumoniae strains from an outbreak in Venezuela: a retrospective study |
| title_short | Characteristics of dual carbapenemase-producing Klebsiella pneumoniae strains from an outbreak in Venezuela: a retrospective study |
| title_sort | characteristics of dual carbapenemase producing klebsiella pneumoniae strains from an outbreak in venezuela a retrospective study |
| topic | klebsiella pneumoniae carbapenem-resistant enterobacteriaceae molecular typing venezuela |
| url | https://iris.paho.org/handle/10665.2/52322 |
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